Modifying Enzymes
Invitrogen™ RNase Cocktail™ Enzyme Mix
Endonuclease-free, exonuclease-free, protease-free
Thermo Scientific™ T4 DNA Ligase, HC (30 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Thermo Scientific™ T4 beta-glucosyltransferase
Highly specific and fast T4 β-glucosyltransferase for 5-hmC modification analysis
Invitrogen™ Ambion™ DNase I (RNase-free)
Nonspecific endonuclease that degrades double- and single-stranded DNA and chromatin
Thermo Scientific™ DNase I, RNase-free (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ E. coli DNA Ligase
Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA.
Invitrogen™ Ambion™ RNase H, from E. coli, 10 U/μL
Endonuclease-free, exonuclease-free, protease-free
Invitrogen™ Gateway™ BP Clonase™ II Enzyme mix
Catalyzes in vitro recombination of PCR products to generate entry clones
Invitrogen™ Ribonuclease H
Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA
Invitrogen™ T4 DNA Ligase (1 U/μL)
Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA
Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)
Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.