4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate) is a chromogenic substrate for lipases and esterases. Upon enzymatic hydrolysis, it releases p-nitrophenol, which can be quantified by colorimetric detection at 410 nm as a measure of enzymatic activity. It is used to characterize the activity of various bacterial and mammalian enzymes, including those from Burkholderia and porcine pancreatic lipase.
- Chromogenic substrate for lipases and esterases.
- Allows quantification of enzymatic activity by colorimetric detection of released p-nitrophenol at 410 nm.
- Used to characterize bacterial and mammalian enzymes.
- In vitro, it serves as a substrate for measuring esterolytic activity of porcine pancreatic lipase, Staphylococcus aureus protease, and Burkholderia sp. lipase.