Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp HEPES, Free Acid, 25 KG
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
Teknova 1M MES, pH 5.76. 1000mL, Sterile 2 pack.
MES solution is a zwitterionic buffer used to help maintain pH levels in biochemistry experiments. It has a buffering range of pH 5.8 - 6.5.
Electron Microscopy Sciences Mops, Biological Buffer, 6.5-7.9 pH 100 GR
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged. Desired pH should be specified when ordering.
pH: 6.5-7.9
Bioworld ADA Buffer, 0.2M, pH 6.0, 250 mL
Product DescriptionADA (N-(2-acetamido)iminodiacetic acid) buffer has a concentration of 0.2M and a pH value of 6.0. It has a molecular weight of 190.2 and formula of C6H10N2O5. ADA is a zwitterionic buffer used in biochemistry and molecular biology, such as its use in protein-free medium for supporting chick embryo fibroblasts. It has also been shown to interfere with color development in bicinchoninic acid assay (BCA) for protein quantitation and used in isoelectric focusing of proteins in immobilized pH gradients.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Sigma Aldrich Fine Chemicals Biosciences MES solution suitable for500ML
MES SOLUTIONon suitable for500ML
Sigma Aldrich Fine Chemicals Biosciences BES sodium salt 99 titrati500G
BES sodium salt 99 titrati500G
Sigma Aldrich Fine Chemicals Biosciences MES hydrate 99.5 titration50KG
MES is one of the Good buffers the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes.
Sigma Aldrich Fine Chemicals Biosciences MOPS sodium salt BioPerfor500G
MOPS sodium salt BioPerfor500G
Bioworld BES Buffer, 0.5M, pH 7.5, 100 mL
Product DescriptionBES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) buffer has a concentration of 0.5M and a pH value of 7.5. BES is a zwitterionic buffer used in biochemistry and molecular biology, and has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES was used to determine that saline buffers minimize the action of interfering factors in the bacterial endotoxins test.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hemisodium | 117961-21-4 | 98.0% | 100 G
MES (2-Morpholinoethanesulphonic acid) hemisodium is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely utilized in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- It is a zwitterionic buffer and provides effective buffering capacity around pH 6.15.
- The pKa value changes with temperature, requiring consideration for its use at different temperatures.
- It shows good buffering properties in biological experiments.
- It exhibits poor affinity with metal ions, allowing its use in studies involving metal ions.
Medchemexpress LLC MOPS | 1132-61-2 | 100.0% | 1 ML
MOPS is a buffering agent utilized in biological applications. It effectively maintains the pH of mammalian cell culture media and significantly enhances the thermal stability of BSA (Bovine Serum Albumin).
- Buffering agent for biological applications.
- Maintains pH of mammalian cell culture media.
- Enhances thermal stability of BSA.
Medchemexpress LLC 1-Piperazinepropanesulfonic acid, β-hydroxy-4-(2-hydroxyethyl)- | 68399-78-0 | 99.9% | 10 G
HEPPSO is a zwitterionic buffer. It has a working pH range of 7.1-8.5. HEPPSO demonstrates a relatively high capacity to bind copper(II) and has a pKa of 7.84 at a 2.0 mM buffer concentration.
- Zwitterionic buffer
- Working pH range of 7.1-8.5
- High capacity to bind copper(II)
- pKa of 7.84 at 2.0 mM buffer concentration
Research Products International Corp HEPES Buffered Saline Solution 2X, 500 Milliliters
2X Formulation:
KCI: 10 mM
Na2HPO4: 1.5 mM
Dextrose: 12 mM
HEPES: 50 mM
pH: 7.0