Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Thermo Scientific™ Wash Buffer for GeneJET Gel Extraction Kit
Thermo Scientific GeneJET Wash Buffer is a component of the GeneJET Gel Extraction Kit and may be purchased separately. Provided in 45 mL aliquots.
Molecular Grade 10X TAE Buffer, Promega
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Thermo Scientific™ TBS Buffer
Tris Buffered Saline (TBS) Buffer is supplied as a lyophilized powder and is intended for use with PanPath products.
Tris-Glycine-SDS Buffer, 10X Solution, Molecular Biology Grade, Hoefer™
CAS: 56-40-6 Molecular Formula: C2H5NO2 Molecular Weight (g/mol): 75.07 MDL Number: MFCD00008131 InChI Key: DHMQDGOQFOQNFH-UHFFFAOYSA-N PubChem CID: 750 ChEBI: CHEBI:15428 IUPAC Name: 2-aminoacetic acid SMILES: NCC(O)=O
Tris Base, Molecular Grade, Promega™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Tris-HCl, Molecular Grade, Promega™
CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
PBS, 10X Solution, Molecular Biology Grade, pH 7.4, Hoefer™
Dilute to 1X with high purity H2O for a solution consisting of 9.5 mM phosphate, pH 7.4, 2.7 mM KCl and 137 mM NaCl
Fisher Science Education™ Tris(hydroxymethyl) Aminomethane
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: THAM,Tris PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Edvotek™ Electrophoresis Buffers
Buffers for use in the science classroom.
Tris Hydrochloride, Ultrapure, 500 g, Innovating Science™
CAS: 1185-53-1 Molecular Weight (g/mol): 157.6
![G-Biosciences Phosphate Buffered Saline (PBS), Sterile, [1X]](https://assets.fishersci.com/TFS-Assets/CCG/G-Biosciences/product-images/PBS_buffer.jpg-250.jpg)
G-Biosciences Phosphate Buffered Saline (PBS), Sterile, [1X]
1X PBS (Phosphate Buffered Saline) is a ready-to-use, sterile, pH adjusted blend of phosphate buffer saline.