Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific TRIS Glycine SDS Buffer, 10X Powder, 184 Grams of Powder, Makes 1 Liter of Solution
TRIS Glycine SDS Buffer (10X Powder) is a commonly used buffer solution in electrophoresis and protein separation techniques. This 184g powder is designed to make 1 liter of 10X concentrated solution, ideal for preparing protein gels for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). The buffer helps maintain a consistent pH and conductivity during protein separation, ensuring reproducible results. It is widely used in laboratories for protein analysis, molecular biology applications, and biochemistry experiments. TRIS Glycine SDS Buffer is essential for the proper functioning of electrophoresis systems, helping researchers isolate proteins based on size and charge in a reliable and efficient manner.
![Alkali Scientific DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661850.jpg-250.jpg)
Alkali Scientific DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters
DPBS 1X (Dulbecco's Phosphate Buffered Saline) is a sterile saline solution used in biological research and clinical laboratories. The 500mL bottle is a convenient size for smaller laboratories or specific experiments that require moderate volumes of buffer solution. DPBS is widely used for washing cells, preparing tissues, and diluting reagents in cell culture, molecular biology, and microbiology applications. The balanced composition of salts and phosphate ensures that the solution maintains osmotic pressure and pH stability, making it essential for maintaining cell viability and supporting biological assays that require precise environmental conditions.
Teknova 10mM Tris-HCl, pH 8.0, 20mL in 50mL Tube, 25 Tubes per Pack, DNase/RNase/Protease Tested, Sterile. 4 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Quality Biological Inc DPBS 10X without Calcium and Magnesium, 500 mL
DPBS is a balanced salt solution ¿ Used for the handling and culturing of mammalian cells. ¿ DPBS without Ca and Mg can be used to wash and rinse suspended cells. ¿ Without Phenol Red ¿ Serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Diluent of fresh red blood cells to study the trapping of red blood cell aggregate ¿ Wash the wells of microtiter plates. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.5, 500 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :pKa (25 °C) :7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.5
Bioworld ADA Buffer, 0.2M, pH 6.0, 100 mL
Product DescriptionADA (N-(2-acetamido)iminodiacetic acid) buffer has a concentration of 0.2M and a pH value of 6.0. It has a molecular weight of 190.2 and formula of C6H10N2O5. ADA is a zwitterionic buffer used in biochemistry and molecular biology, such as its use in protein-free medium for supporting chick embryo fibroblasts. It has also been shown to interfere with color development in bicinchoninic acid assay (BCA) for protein quantitation and used in isoelectric focusing of proteins in immobilized pH gradients.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Bioworld ACES Buffer, 0.5M, pH 7.5, 500 mL
Product DescriptionACES (N-(2-acetamindo)-2-aminoethancesulfonic acid) buffer has a concentration of 0.5M and a pH level of 7.5. Buffer was prepared in 18.2 megohms water and filtered through 0.22 micron filter. ACES has been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. A saturated solution contains 0.22M at 0°C and a melting point greater than 220°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. Product is stored at room temperature.
General Laboratory Products 2mL 0.85 per. Saline, 100/cs
2mL 0.85 per. Saline with Shelf Life of 1 yr.
Alkali Scientific TRIS Base, 40% Solution, 4 Liter
TRIS Base, 40% Solution (4 liters), is a buffered solution widely used in biochemical and molecular biology laboratories. This 40% solution offers a convenient, ready-to-use concentration of Tris Base, ideal for buffer preparation and pH stabilization in various applications. The solution can be diluted as needed to create custom concentrations for specific experiments. TRIS Base is used in a variety of applications including protein electrophoresis, nucleic acid analysis, and enzyme assays. The buffered solution maintains a consistent pH environment, which is essential for ensuring reliable and reproducible results in molecular biology protocols and analytical experiments.
Dojindo Molecular Technologies Inc MOPS (25G), Good's buffer, CAS: 1132-61-2, pka=7.2, Active pH 6.5-7.9, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Alkali Scientific CellPro™ TBE 10X (Tris-Borate EDTA), 1L
Alkali Scientific™ CellPro™ TBE (Tris-borate EDTA) solution offers a sterile and filtered formulation of this essential buffer for molecular biology and electrophoresis applications. With its meticulous preparation, this solution ensures purity and consistency, meeting the rigorous demands of scientific research and experimentation. The sterile nature of the solution minimizes the risk of contamination, making it ideal for various molecular biology techniques, including DNA and RNA agarose gel electrophoresis. Additionally, the filtered formulation guarantees clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used for nucleic acid separation, DNA fragment analysis, or other molecular biology procedures, CellPro™ TBE solution delivers consistent performance and reproducible results, supporting the success of laboratory workflows.
Alkali Scientific CellPro™ PBS Buffer, with Ca + Mg, 1X, pH 7.4, 500mL
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Alkali Scientific CellPro™ TE, 1X (Tris EDTA), pH 8.0, 100mL
Introducing CellPro™ TE 1X (Tris EDTA), meticulously formulated by Alkali Scientific™ to meet the exacting standards of molecular biology research. This Molecular Biology Grade TE buffer ensures exceptional purity and performance, making it an indispensable tool for various molecular biology applications, including DNA and RNA purification, DNA sequencing, and PCR. With its precisely balanced composition and stringent quality control measures, CellPro™ TE 1X provides researchers with reliable and reproducible results, facilitating accurate and efficient laboratory workflows. Trust CellPro™ TE 1X for your molecular biology experiments and experience the assurance of superior quality and performance in every assay.
Alkali Scientific QuickSilver PBS-T Buffer Powder, 100 Pouches
QuickSilver™ Buffer Powder packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, and easy to use. Convenient premeasured pouches make 1L of 1X buffer. There is no pH adjusent needed and they offer a long shelf life. They come supplied with preprinterd bottle labels for easy identification. 100 pouches per pack.