Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
eMolecules 1363382-91-5 | tert-butyl 3-(hydroxymethyl)-3-methylazetidine-1-carboxylate | MFCD20926177 | 1g
ChemScene | 5-Bromo-1H-pyrazole-4-carbonitrile | 100mg | 642098150 | CS-0196853 | 1703808-52-9 | MFCD28519385 | 171.985 | C4H2BrN3
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![eMolecules 1330764-06-1 | tert-butyl 7-(hydroxymethyl)-5-oxa-2-azaspiro[3.4]octane-2-carboxylate | MFCD20230641 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502158274.JPG-250.jpg)
eMolecules 1330764-06-1 | tert-butyl 7-(hydroxymethyl)-5-oxa-2-azaspiro[3.4]octane-2-carboxylate | MFCD20230641 | 1g
Medchem Express | SG3199 | 1mg | 649421525 | HY-101161 | 1595275-71-0 | 584.673 | C33H36N4O6
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Teknova TMN200 Solution, pH 8.0, Research Grade. 2L, Sterile 2 Pack.
200mM Sodium Chloride, 1mM Magnesium Chloride,20mM Tris
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 9.0) - 2x1L
Bioprocess Grade - Tris-HCl Buffer (1 M pH 9 0) - 2x1L (For Research Use and Further Manufacturing Only)
eMolecules 1179360-20-3 | TERT-BUTYL 3-(HYDROXYMETHYL)-1,4-DIAZEPANE-1-CARBOXYLATE HCL | MFCD23702017 | 1g
Ambeed | (R)-Oxiran-2-ylmethyl 4-nitrobenzenesulfonate | 1g | 628567785 | A635502 | 123750-60-7 | MFCD08460169 | 259.230 | C9H9NO6S
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Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-12 Bis-Tris Preca
mPAGE 4-12% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
eMolecules 158144-85-5 | TERT-BUTYL 4-(HYDROXYMETHYL)-4-PHENYLPIPERIDINE-1-CARBOXYLATE | AstaTech | MFCD11044987 | 291.391 | C17H25NO3 | 95.000 | CC(C)(C)OC(=O)N1CCC(CO)(CC1)c1ccccc1 | 1g | 206684235
TERT-BUTYL 4-(HYDROXYMETHYL)-4-PHENYLPIPERIDINE-1-CARBOXYLATE | AstaTech | 158144-85-5 | MFCD11044987 | 291.391 | C17H25NO3 | 95.000 | CC(C)(C)OC(=O)N1CCC(CO)(CC1)c1ccccc1 | 1g | 206684235
Bioworld TT Buffer, Ready-Pack (10X), 2 L
Tris-Tricine BufferTris-Tricine (TT) Buffer has been developed for both non-denaturing and denaturing electrophoresis of proteins, and offers several advantages over more conventionally used buffers. TT Buffer allows for optimal resolution of low molecular weight proteins and elimantes the glycine front often found in gel utilizing TG-SDS and other such beffers. This improved running buffer allows for resolution of peptides as low as 5kD. TT buffer is also excellent for protein electrophoresis where resolved products will be transferred to a nylon membrane. Unlike glycine, tricine will not interfere with membrane protein sequencing. The 10X pack just requires the addition of distilled H2O to make 1.0 Liter of 10X liquid. A simple 10-fold dilution with water provides a 1X solution, that makes an excellent running buffer for most applications. Technical Information:Ultra PureGradeStorage: RTHazard: Irritant
eMolecules 1184985-22-5 | tert-butyl 3-(hydroxymethyl)-4-methyl-pyrrolidine-1-carboxylate | MFCD08461237 | 1g
ChemScene | 3-Hydroxy-5-(trifluoromethyl)benzonitrile | 100mg | 712788143 | CS-0373293 | 1243459-56-4 | MFCD16999150 | 187.121 | C8H4F3NO
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![Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 Liter Cube Container](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661290.jpg-250.jpg)
Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 Liter Cube Container
TBE 10X Solution is a highly effective electrophoresis buffer solution, formulated with Tris, borate, and EDTA. This 4-liter cube container provides a concentrated 10X solution that is commonly used for gel electrophoresis of nucleic acids. TBE helps maintain a consistent pH and ionic strength, critical for the accurate migration of DNA and RNA during electrophoresis. The 10X concentration allows for easy dilution to a working concentration of 1X, making it an ideal buffer for high-throughput applications in molecular biology. This large container is perfect for laboratories that perform a high volume of gel electrophoresis and other nucleic acid analysis procedures.
![eMolecules 1365570-27-9 | tert-butyl 5-(hydroxymethyl)-octahydrocyclopenta[c]pyrrole-2-carboxylate | Pharmablock | MFCD22415193 | 241.331 | C13H23NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC(CO)CC2C1 | 1g | 551087381](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502157040.JPG-250.jpg)
eMolecules 1365570-27-9 | tert-butyl 5-(hydroxymethyl)-octahydrocyclopenta[c]pyrrole-2-carboxylate | Pharmablock | MFCD22415193 | 241.331 | C13H23NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC(CO)CC2C1 | 1g | 551087381
tert-butyl 5-(hydroxymethyl)-octahydrocyclopenta[c]pyrrole-2-carboxylate | Pharmablock | 1365570-27-9 | MFCD22415193 | 241.331 | C13H23NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC(CO)CC2C1 | 1g | 551087381
eMolecules 906673-45-8 | 4-(4-Bromo-3-(hydroxymethyl)phenoxy)benzonitrile | MFCD12165918 | 1g
Ambeed | 4-(4-Bromo-3-(hydroxymethyl)phenoxy)benzonitrile | 1g | 525113864 | A197114 | 906673-45-8 | MFCD12165918 | 304.143 | C14H10BrNO2
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