Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Chem-Impex International, Inc. 3-Cyclohexylamino-1-propanesulfonic acid | 1135-40-6 | MFCD00003837 | 100G
3-Cyclohexylamino-1-propanesulfonic acid, 1135-40-6, MFCD00003837, 100G
Alkali Scientific Murashige & Skoog Medium with MES Buffer, 240 Grams of Powder, Makes 50 Liters of Solution
Murashige & Skoog (MS) Medium with MES Buffer is a powdered medium formulated for plant tissue culture applications. The 240g powder makes 50 liters of nutrient solution, enriched with MES buffer to stabilize the pH during plant cell culture. The inclusion of MES ensures better pH stability during long incubation periods, especially in environments where pH fluctuations can affect cell growth. This medium is ideal for research in plant biotechnology, as it supports both the growth and differentiation of plant cells, tissues, and organs. It is commonly used for in vitro studies, propagation, and transformation of various plant species.
Medchemexpress LLC L-Adenosine | 3080-29-3 | 99.8% | 1 ML
L-Adenosine is a metabolically stable enantiomeric analog and a potential probe. It exhibits weakly inhibitory adenosine deaminase (ADA) activity with a Ki value of 385 μM. This compound can be utilized for research into adenosine uptake and accumulation.
- Metabolically stable enantiomeric analog.
- Potential probe for research.
- Weakly inhibits adenosine deaminase activity.
- Can be used for adenosine uptake research.
- Can be used for adenosine accumulation research.
Medchemexpress LLC MOPS | 1132-61-2 | 99.95% | 10 G
MOPS is a buffering agent used in biological applications. It functions to maintain the pH of mammalian cell culture media. Additionally, it has been shown to significantly enhance the thermal stability of BSA.
- Functions as a buffering agent in biology.
- Maintains the pH of mammalian cell culture media.
- Significantly improves the thermal stability of BSA.
Medchemexpress LLC Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)-, sodium salt (1:2) | 41689-31-0 | 98.0% | 25 G
ADA disodium is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, especially in the preparation of enzyme buffers.
- Derived from adenosine deaminase
- Used in biochemical research
- Ideal for enzyme buffer preparation
Medchemexpress LLC HEPES hemisodium salt | 103404-87-1 | MFCD00036463 | 99.0% | 249.80 | C8H18N2O4S·1/2Na | 100 MG
HEPES hemisodium salt is a zwitterionic buffering reagent commonly used to maintain physiological pH in biochemical assays and cell-culture media. Supplied as a solid powder, it is intended for preparation of HEPES buffer solutions and includes storage and handling guidance for laboratory use.
- High buffering capacity at physiological pH (6.8-8.2).
- Available as a solid powder for convenient solution preparation.
- Reported high purity suitable for analytical and cell-culture applications.
- Stable with sealed storage at 4°C and protected from moisture.
- Provided with supporting documentation (datasheet and safety information).
Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate (Standard) | 145224-94-8 | 99.95% | 50 MG
MES monohydrate (Standard) is the analytical standard of MES (monohydrate), intended for research and analytical applications. MES (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely used in biochemistry and molecular biology experiments.
- Used for research and analytical applications.
- Amphoteric ion buffer.
- Buffer capacity ranging pH 5.5-7.0.
- Widely used in biochemistry and molecular biology experiments.
- Suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Commonly used in qualitative, quantitative, and methodological research experiments in HPLC, GC, and MS.
LICORbio Odyssey MES SDS Running Buffer Powder (pack of 5)
Odyssey Mini Gels are polyacrylamide gels designed to separate a wide range of protein sizes via PAGE or SDS-PAGE. Each box contains 10 pre-cast gels. Our gels feature larger well volumes for easier sample loading and are cast in a neutral pH buffer to increase gel stability. They also run at neutral pH, which minimizes protein modification compared to Tris-glycine gels. Odyssey Mini Gels are available in several gradients with 10, 12, or 15 numbered wells. Our gels feature a shelf life of up to 6 months at room temperature and 12 months at 2–8C.
Boston Bioproducts Inc PIPES Buffer (0.5 M, pH 7.0) - 100ml
The PIPES Buffer is a zwitterionic buffering agent commonly used in biochemistry and molecular biology for its ability to maintain a stable pH. With a pKa of approximately 6.8, PIPES is effective in the pH range of 6.1 to 7.5, making it suitable for cell culture, enzyme assays, protein purification, and as a running buffer in gel electrophoresis. PIPES is also used in electron microscopy, protein crystallization, and isoelectric focusing and chromatography.
Hampton Research HR2-243/StockOptions MES, pH 5.2 to 7.1 in 0.1 pH unit increments - 10 ml, tube format
HR2-243/StockOptions MES, pH 5.2 to 7.1 in 0.1 pH unit increments - 10 ml, tube format. Please note items from this supplier are non-returnable.
Medchemexpress LLC 1-Piperazinepropanesulfonic acid, β-hydroxy-4-(2-hydroxyethyl)- | 68399-78-0 | 99.9% | 50 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It has a pKa of 7.84 at 2.0 mM buffer concentration and exhibits a relatively high ability to bind copper(II). It is for research use only.
- Zwitterionic buffer
- Working pH range: 7.1-8.5
- pKa of 7.84 at 2.0 mM buffer concentration
- Relatively high ability to bind copper(II)