Recirculating Chillers
Sigma Aldrich Fine Chemicals Biosciences Denaturation Solution for
Sigmas denaturing SOLUTIONon is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination SOLUTIONon treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination allowing for more efficient transfer of large DNA in neutral Southern transfers.
Sigma Aldrich Fine Chemicals Biosciences Tetramethylrhodamine isoth1G
methyl Tetramethylrhodamine isoth1G
Sigma Aldrich Fine Chemicals Biosciences TrizmaR hydrochloride solu1L
A series of Pre-mixed SOLUTIONons of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy /- 0.1 pH units.
Sigma Aldrich Fine Chemicals Biosciences AeraSealTM film non-steril
A 140 mum thick hydrophobic porous sealing film with medical-grade adhesive for tissue culture plates bio-blocks and 96-well plates where gas exchange is necessary for cell or bacterial growth. AeraSeal(TM) sealing films minimize cross-contamination spillage and evaporation. They allow uniform air and CO2 exchange for all wells unlike plate lids which favor exchange for wells near the plate edges. Dimensions 82.6 by 142.9 mm for standard-size tissue culture platenon-cytotoxic highly gas permeable easily pierceable with pipet tips or pipets for sample recovery recommended for temperatures from -20 C to 80 CNote AeraSeal(TM) films areAsuitable for hypoxic culture conditions.
Sigma Aldrich Fine Chemicals Biosciences Tetramethylrhodamne soth100MG
methyl Tetramethylrhodamne soth100MG
Sigma Aldrich Fine Chemicals Biosciences Antonia RedTM Dextran 1505MG
Antonia Red(TM) dextrans are purple powders that are readily soluble in water or electrolyteSOLUTIONons. Antonia Red dextran are insoluble in most organic solvents.
Sigma Aldrich Fine Chemicals Biosciences TRI ReagentR for processin
TRI reagent(R) is a quick and convenient ready-to-use reagent useful for efficient total RNA extraction or for the simultaneous isolation of RNA DNA and protein. TRI reagent(R) is a mixture of guanidine thiocyanate and phenol in a monophasic SOLUTIONon. Homogenization or lysis of the tissue sample in TRI reagent(R) dissolves RNA DNA and protein. The addition of chloroform or 1-bromo-3-chloropropane followed by centrifugation results in the separation of the mixture into three phases an aqueous phase containing the RNA the interphase containing DNA and an organic phase containing proteins. Each of the components can then be isolated after separating the phases.
Sigma Aldrich Fine Chemicals Biosciences TrizmaR hydrochloride solu1L
Tris hydrochloride is used to prepare buffer mainly at a physiological range of 7.3 to 7.5. This buffer is well-suited with biological fluids. It is used in laboratories as primary pH standard in clinical laboratories.
Sigma Aldrich Fine Chemicals Biosciences HiTrapR Heparin High Perfo
HiTrap(R) Heparin HP columns are prepacked with Heparin Sepharose(R) High Performance for high-reSOLUTIONon purification of proteinswith affnity forheparin such as DNA-binding proteins coagulation factors lipoproteins and protein synthesis factors.Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules including coagulation factors and other plasma proteins lipoproteins protein synthesis factors enzymes that act on nucleic acids and steroid receptors. Heparin also functions as a high capacity cation exchanger due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.Heparin is coupled to the Sepharose(R) High Performance base matrix via the N-hydroxysuccinamide coupling method to give high capacity performance and low leakage levels. HiTrap(R) Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap(R) H
Sigma Aldrich Fine Chemicals Biosciences Omnipore Membrane filter 1
Our fluoropore(R) membrane is a hydrophobic polytetrafluoroethylene (PTfE) polymer membrane bonded to a high density polyethylene support to improve the handling characteristics of the filter for normal applications. fluoropore membranes provide broad chemical compatibility high flow rates and consistency. (Please note that catalogue numbers containing fHUP are unlaminated or produced without a backing.)Our Omnipore membrane is a hydrophilic PTfE membrane that is compatible with virually all solvents acids and alkaline SOLUTIONons.features Benefits - Biologically and chemically inert - High porosity yields high flow rates - Hydrophobic and hydrophilic PTfE varieties available for filtration of aqueous and organic-based samples Applications - fluoropore Membrane Clarifying Acids Bases and Solvents Air Monitoring filtering and Venting Gases UV Spectroscopy - Omnipore Membrane filtration of Aqueous SOLUTIONons Clarifying Acids and Alkaline SOLUTIONon
Sigma Aldrich Fine Chemicals Biosciences KiCqStartR SYBRR Green qPC
KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated ready-to-use reaction cocktail that contains all components except primers and template for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer stabilizers and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency sensitivity specificity and robust fluorescent signal using fast or conventional cycling protocols with SYBR Green qPCR.Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. The optimized formulation includes SYBR Green I dye an antibody-mediated Hot-Start Taq DNA polymerase dNPTs MgCl2 and proprietary buffers and stabilizers.
Med Vet International 3mL (3Cc) Luer Lock Syringe With Safety Needle, 25g X 1.5in, 50/Box
3mL (3cc) Luer Lock Syringe With Safety Needle, 25G X 1.5in, 50/Box Safety Needle Features One handed activation No solid surface required Simple finger stroke activation Protective arm keeps fingers behind the needle An audible "click" as well as tactile feel and a visual indicator confirm safety mechanism engagement Needle remains locked inside activated cover Fits any brand Syringe
Julabo USA Inc Each, FL2506 chiller, -15+40C, 2.5 kW, 230V/60Hz
Chillers, Julabo, FL2506 Recirculating Cooler -15…+40 °C., 2.5 kW cooling power; ±0.5°C stability. Proportional cooling control adjusts the cooling power to the load of the application saving energy. Units incorporate RS232 interface and alarm output for PC integration and control. Models include a splash-proof membrane keypad with LED temperature display. Easy filling / draining with a fill level indicator and overflow port on the back. Units constructed of stainless steel and high-grade plastic with stainless steel internal tank. Centrifugal pressure pump. Units with 3 and 6 bar pump include internal by-pass for pressure adjustment with analog pressure gaugeMay be used with water, water/glycol mixtures and silicone fluids.The FL300, FL601, FL1201, FL1701, FLW1701 come with 8 and 12 mm hose barbs. The FLxxx3 models come with 3/4″ hose barbs. The FLxxx6 models come with 1″ hose barbs. (pump connections with G 1 1/4" male)