Protein Method Blocking Buffers
Bioworld Blotto in TBS, 1 L
Blotto in TBS Blot blocking buffer used to block non-specific binding for antibodies on the membrane Technical Information: 5% nonfat dry milk, 0.02% Sodium azide in TBS
Bioworld Denhardt's Solution (100X), 50 mL
Denhardt's Solution, 100X Concentrate Acts as a blocking reagent in hybridizations to help prevent probe binding to open membrane space and non-target nucleic acids. Specifications:Ultra Pure GradePurity: 99.0%pH @ 25°C: 5.0-7.0 Conductivity @ 25°C (µmhos): <350 Abs @ 278nm (5% water): 0.600-0.750 DNAse: None detectedRNAse: None detectedStorage: -15 to -22°C
SCANTIBODIES LABORATORY INC HBR 1, PURIFIED (3KC533) 500MG
The 3KC533 Heterophilic Blocking Reagent 1, Purified from Scantibodies Laboratory, Inc. contains specific murine immunoglobulins that block the heterophilic interaction by active binding to the heterophilic antibodies, which are capable of cross-linking the capture and the detection antibodies used in the immunoassay, resulting in false positive readings. The attachment of HBR-1 to the heterophilic antibodies blocks this cross-linking, and eliminates the interference caused by the heterophilic antibodies in the humoral fluids. In addition to its active blocking, this product is also characteristic of its passive blockage of the heterophilic interaction as well. The HBR is a liquid reagent with a protein concentration of 20 ± 2 mg/ml. The immunoglobulins are dissolved in a phosphate buffer with a pH of 7.2-7.6. The immunoglobulins in this product are at a purity of greater than or equal to 95%.
Research Products International Corp PBS/Tween 20 10X Solution, 500 Milliliters
PBS/Tween 20 10X Solution is a widely used buffer solution in the life science marketplace. It is a combination of phosphate-buffered saline (PBS) and Tween 20, a nonionic detergent.
Bioworld Blotto/Antifoam in TBS, 500 mL
Blotto/Antifoam in TBS Blot blocking buffer used to block non-specific binding site for antibodies on the membrane Technical Information: 5% nonfat dry milk, 0.01% Anti-Foam A, 0.02% Sodium azide in TBS
Alkali Scientific TBS / Tween 20 10X Solution, 500 Milliliters
TBS / Tween 20 10X Solution is a highly effective washing buffer used to minimize background noise during immunodetection procedures such as Western blotting and ELISA. This 500mL solution is ideal for smaller-scale applications or as a concentrate that can be diluted for use in various experimental setups. The Tris component provides pH stability, while Tween 20 aids in reducing non-specific binding during protein-protein interactions. This formulation ensures cleaner results by washing away excess proteins and antibodies that do not bind specifically to the target. TBS-Tween 20 is essential for improving the signal-to-noise ratio in sensitive immunoassays and diagnostic tests.
Cell Biolabs Inc PhosphoBLOCKER™ Blocking Reagent, 200 g dry blend (4 Liters prepared)
The utilization of anti-phosphoprotein antibodies in western blotting has become a commonly used tool for signal transduction research. Unfortunately, low levels of endogenous phosphoprotein in various cell lysates often can not be detected, even with high concentrations of antibody and long exposure times. Most commercially available western blot blockers (e.g. dry milk, serum) are sufficient to block the unreacted sites on the membrane, reducing the amount of nonspecific antibody binding during the assay; however, they are not designed to preserve phosphoprotein antigens during blotting. Cell Biolabs’ PhosphoBLOCKER™ contains a proprietary formulation that provides several advantages over conventional blockers: (1) It is designed specifically for phosphoprotein blotting; (2) It enhances low level phosphoprotein signal without increasing background; and (3) it is an easy to use premixed dry blend. 200 grams (reconstituted to 4 liters).
Alkali Scientific TBS / Tween 20 10X Solution, 1 L
TBS / Tween 20 10X Solution is a powerful washing buffer used in a variety of biological and biochemical assays. This 1L solution is ideal for applications in immunohistochemistry, Western blotting, and enzyme-linked immunosorbent assays (ELISA). The formulation includes Tris, which maintains a stable pH, and Tween 20, a non-ionic detergent that helps reduce non-specific binding by blocking protein interaction sites. This buffer is essential for washing steps during immunodetection procedures, ensuring that only the specific antigen-antibody complex remains bound while unwanted proteins are removed. Its versatility makes it a staple in molecular biology, diagnostic laboratories, and research environments.
Quality Biological Inc DPBS with TWEEN 20 without Ca & Mg, 1000 mL
DPBS can be used to provide a buffer system to maintain cell culture media in the physiological pH range of 7.0 to 7.6. ¿ DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ DPBS is also used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate, to wash the wells of microtiter plates, and to wash cells during preparation and serial transfer. ¿ 1X working solution is recommended ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Medchemexpress LLC 1,1'-(4,4'-(((5-chloropyrimidine-2,4-diyl)bis(azanediyl))bis(3-methoxy-4,1-phenylene))bis(piperazine-4,1-diyl))diethanone | 1472795-20-2 | MFCD28133395 | 96.2% | 609.12 g·mol^-1 | C30H37ClN8O4 | 10MM 1ML
KRCA-0008 is a selective dual inhibitor of anaplastic lymphoma kinase (ALK) and activated Cdc42-associated kinase 1 (ACK1/TNK2) with reported IC50 values of approximately 12 nM for ALK and 4 nM for ACK1. It is intended for preclinical research into oncogenic signalling and cancer biology and is supplied as a concentrated DMSO solution suitable for in vitro biochemical and cellular assays.
- Potent dual kinase inhibition with low nanomolar IC50 values.
- Suitable for biochemical and cellular assays.
- Provided as a 10 mM solution in DMSO for convenient dosing.
- High purity (≈96.2%) with stated stability and storage recommendations.
- Intended for research use only; not for human consumption.
IBI SCIENTIFIC 3P 10X PBST (PBS W/0.5% TWEEN 20) 4/PACK
Sterile buffer solution containing sodium phosphate, sodium chloride, potassium phosphate and potassium chloride with 0.5% TWEEN 20.Sterile filtered using 0.1um filterDNase, RNase and protease free.Sterility validated using USP <71> Test for SterilityDoes not contain calcium, magnesium, phenol red or sodium pyruvate. IBI 10X PBST (10X PBS) with TWEEN 20 (0.5% TWEEN 20) is a concentrated sterile solution in a 1 liter bottle that is ideal for use in immunoassay procedures such as Western Blotting and ELISAs in microtiter plate wells.IBI 10X PBST is mixed and bottled in our liquid production facility here in Dubuque, Iowa. Our high output pure water system produces endotoxin free and nuclease free water at a rate of 100 gallons per/hr. This water is the basis for all of our liquid blending production here at IBI.