Protein Method Blocking Buffers
Teknova 20X PBS with 1% Tween-20. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Surmodics Inc StabilBlock Immunoassay Stabilizer, 1L
StabilBlock Immunoassay Stabilizer effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. It can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper. The key benefits are reduction of non-specific binding while increasing signal-to-noise ratios within immunoassay applications.
Bio Rad Laboratories EveryBlot Blocking Buffer, 500 mL
EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.
LICOR Intercept (TBS) Blocking Buffer (3 x 500 mL)
Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies; it can be used with the widest range of targets for Western blot detection; it is ideal for primary antibodies that are optimized for Tris-based buffer systems; it can be used for detection of phosphorylated targets; it has superior performance for alkaline phosphatase detection; it is an excellent choice for biotin-streptavidin detection.Shake well before each use.
Vector Laboratories Mouse On Mouse (M.O.M.) Blocking Reagent 1 ml
M.O.M. Mouse Ig Blocking Reagent is used to block endogenous mouse antibody in tissue sections. A component of the M.O.M. Kits, this reagent provide optimal staining results when it is paired with the M.O.M. Biotinylated Anti-Mouse Ig Reagent or the biotin-free, one-step micropolymer detection reagent, ImmPRESS Anti-Mouse Ig Reagent (MPX-2402). This reagent is supplied as a 1 ml concentrate, sufficient to prepare about 25 ml of working solution, or enough to stain 125-250 sections.
Biotium TrueBlack Plus Lipofuscin Autofluorescence Quencher | 500uL
TrueBlack Plus Lipofuscin Autofluorescence Quencher | 40x concentrated solution in DMSO | 500uL
LICOR Intercept T20 (TBS) Antibody Diluent (500 mL)
Intercept T20 (TBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Nacalai USA Inc BLOCKING ONE, 500ML
Blocking One is superior to conventional blocking solutions. The preservative does not affect the enzyme activity of POD or ALP. Only simple refrigerator storage is necessary, even after opening the bottle.
Teknova 10mM Tris-HCl with 0.1% Tween-20, pH 8.5. 1mL/Tube, 50 Pack, Sterile.
10mM Tris-HCl, 0.1% Tween-20
Sigma Aldrich Fine Chemicals Biosciences Carbonate-Bicarbonate Buffer capsule | 50CAP
Carbonate-Bicarbonate Buffer capsule | 50CAP
Biotium 10X Fish Gelatin Blocking Agent
10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.
Active Motif MAXblock™ Blocking Medium
MAXblock Blocking Medium is a protein-based, non-mammalian blocking agent for use in immunofluorescence and immunostaining assays. Superior blocking is achieved utilizing a protein blend that demonstrates no cross-reactivity with secondary antibodies. It has been proven effective at blocking non-specific binding of antibodies in immunofluorescence, as well as in immunohistochemistry.
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline with 0.05% TWEEN 20, pH 7.4, 10 Pack
Phosphate Buffered Saline with 0.05% TWEEN 20, pH 7.4, 10 Pack