Protein Method Blocking Buffers
BLOTTO antifoam in PBS, with 0.02% sodium azide, Thermo Scientific™
Blocking reagent for immunohistochemistry experiments
Azure Biosystems Chemi Blot Blocking Buffer
Blocking solution for chemiluminescent Western blot, 1X Solution
Azure Biosystems Protein-Free Blot Blocking Buffer
Protein-free blocking solution for Western blot, 5X concentrate
TBST (Tris-Buffered Saline + Tween 20), 20X Solution, Molecular Biology Grade, Hoefer™
Dilute to 1X with high purity H20 for a solution consisting of 25mM Tris, pH 7.4, 3.0mM KCl, 140mM NaCl and 0.05% Tween™ 20
SCANTIBODIES LABORATORY INC HBR 1, PURIFIED (3KC533) 500MG
The 3KC533 Heterophilic Blocking Reagent 1, Purified from Scantibodies Laboratory, Inc. contains specific murine immunoglobulins that block the heterophilic interaction by active binding to the heterophilic antibodies, which are capable of cross-linking the capture and the detection antibodies used in the immunoassay, resulting in false positive readings. The attachment of HBR-1 to the heterophilic antibodies blocks this cross-linking, and eliminates the interference caused by the heterophilic antibodies in the humoral fluids. In addition to its active blocking, this product is also characteristic of its passive blockage of the heterophilic interaction as well. The HBR is a liquid reagent with a protein concentration of 20 ± 2 mg/ml. The immunoglobulins are dissolved in a phosphate buffer with a pH of 7.2-7.6. The immunoglobulins in this product are at a purity of greater than or equal to 95%.
Boca Scientific Inc SmartBlock™, 500ml
SmartBlock™ is a BSA-free, peptide-based blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces, removing background and preventing unwanted binding. This reagent was produced by chemically modifying peptides in a specific process that resulted in a peptide solution with strong blocking characteristics. SmartBlock™ has high lot-to-lot consistency that had previously only been obtained by synthetic blockers. For many assays, SmartBlock™ is an economical and reliable alternative to BSA-containing solutions. Problems caused by BSA within blockers, such as BSA-interactions and cross reactivities, can be eliminated by using SmartBlock™.
SCANTIBODIES LABORATORY INC HBR PLUS (3KC545) 1.0G
The 3KC545 Heterophilic Blocking Reagent Plus from Scantibodies Laboratory, Inc. was developed as an alternative for HBR-1. This product is compounded with immunoglobulins with different characteristics. Therefore, in addition to its active blocking characteristics, the special formulation and production procedures enhance its efficacy in its passive blocking ability as well. It has a concentration of 10.0 +/- 1.0 mg/ml.
INNOVEX BIOSCIENCES IMMUNODILUENT & BLOCK WITH GREEN COLOR INDICATOR 125 ML
Immunodiluent& Block 125ml MFCD00131855
INNOVEX BIOSCIENCES UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 15 ML
Innovex peptide-based Fc Blocker universally blocks all types Fc receptors (I, II, III) of human, mouse & all animal species; Contains NO antibody, Ig or Ig fragments, is used for IHC, IF & flow cytometry
Bioworld Blotto in TBS, 500 mL
Blotto in TBS Blot blocking buffer used to block non-specific binding for antibodies on the membrane Technical Information: 5% nonfat dry milk, 0.02% Sodium azide in TBS