Protein Method Blocking Buffers
LICOR Intercept (TBS) Blocking Buffer (500 mL)
Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies; it can be used with the widest range of targets for Western blot detection; it is ideal for primary antibodies that are optimized for Tris-based buffer systems; it can be used for detection of phosphorylated targets; it has superior performance for alkaline phosphatase detection; it is an excellent choice for biotin-streptavidin detection.Shake well before each use.
Teknova 20X PBS with 1% Tween-20. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
LICOR Intercept (PBS) Blocking Buffer (500 mL)
Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system. It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies, it is an excellent choice for biotin-streptavidin detection and it is suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon-FL PVDF and nitrocellulose membranes.
LICOR Intercept (TBS) Blocking Buffer (3 x 500 mL)
Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies; it can be used with the widest range of targets for Western blot detection; it is ideal for primary antibodies that are optimized for Tris-based buffer systems; it can be used for detection of phosphorylated targets; it has superior performance for alkaline phosphatase detection; it is an excellent choice for biotin-streptavidin detection.Shake well before each use.
LICOR Intercept T20 (TBS) Antibody Diluent (500 mL)
Intercept T20 (TBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Vector Laboratories Mouse On Mouse (M.O.M.) Blocking Reagent 1 ml
M.O.M. Mouse Ig Blocking Reagent is used to block endogenous mouse antibody in tissue sections. A component of the M.O.M. Kits, this reagent provide optimal staining results when it is paired with the M.O.M. Biotinylated Anti-Mouse Ig Reagent or the biotin-free, one-step micropolymer detection reagent, ImmPRESS Anti-Mouse Ig Reagent (MPX-2402). This reagent is supplied as a 1 ml concentrate, sufficient to prepare about 25 ml of working solution, or enough to stain 125-250 sections.
Bio Rad Laboratories EveryBlot Blocking Buffer, 500 mL
EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.
Surmodics Inc StabilBlock Immunoassay Stabilizer, 1L
StabilBlock Immunoassay Stabilizer effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. It can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper. The key benefits are reduction of non-specific binding while increasing signal-to-noise ratios within immunoassay applications.
Teknova 10mM Tris-HCl with 0.1% Tween-20, pH 8.5. 1mL/Tube, 50 Pack, Sterile.
10mM Tris-HCl, 0.1% Tween-20
Biotium TrueBlack Plus Lipofuscin Autofluorescence Quencher | 500uL
TrueBlack Plus Lipofuscin Autofluorescence Quencher | 40x concentrated solution in DMSO | 500uL
Meridian Life Science TRU Block ULTRA, 0.1 g, Liquid, Immunoassay interference blocker, >95% purity, 24-26 mg/mL protein concentration, 10 mM Sodium Phosphate, 150 mM Sodium Chloride
TRU Block ULTRA, 0.1 g, Liquid, Immunoassay interference blocker, >95% purity, 24-26 mg/mL protein concentration, 10 mM Sodium Phosphate, 150 mM Sodium Chloride
Vector Laboratories Animal-Free Blocker and Diluent (AFB), Ready-to-Use (R.T.U.) 100 ml
R.T.U. Animal-Free Blocker and Diluent is a ready-to-use antibody diluent and blocking reagent that stabilizes diluted antibody solutions and diminishes background due to non-specific binding in immunohistochemical applications. It contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations where these or other traditional blocking agents are ineffective. It is especially convenient for multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.