Western Blot Substrates and Substrate Kits

Western Blot Substrates and Substrate Kits
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Filtered Search Results

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

Shipping Condition | Room Temperature |
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Substrate | Horseradish Peroxidase |
Recommended Antibody Concentrations | 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL) |
Conjugate | HRP |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 6 to 24 hr. |
For Use With (Application) | Western Blot |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate
SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.

Shipping Condition | Wet Ice |
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Substrate | Horseradish Peroxidase |
Sensitivity | Low-femtogram to high-attogram |
Form | Liquid |
Product Type | Substrate |
Membrane Compatibility | Nitrocellulose, PVDF |
For Use With (Application) | Western Blot |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Substrate | Horseradish Peroxidase |
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Sensitivity | Mid-femtogram |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 24 hr. |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Substrate | Horseradish Peroxidase |
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Form | Liquid |
Product Type | Substrate |
Signal Duration | 6 to 8 hr. |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Substrate | Horseradish Peroxidase |
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Content And Storage | Upon receipt store at 2-8°C. Product is shipped at ambient temperature. |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 1 to 2 hr. |
Product Line | Pierce™ |
Detection Method | Chemiluminescence |
Thermo Scientific™ 1-Step™ NBT/BCIP Substrate Solution
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

MilliporeSigma™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

Substrate | Horseradish Peroxidase |
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Content And Storage | 2°C to 8°C |
Shelf Life | 9 Months |
For Use With (Application) | Dot/Slot Blot |
Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

Content And Storage | 2°C to 8°C |
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Kit Contents | Detection reagent 1 and 2 |
Product Type | ECL Western Blot Substrate |
Thermo Scientific™ 1-Step™ Chloronaphthol Substrate Solution
1-Step™ Chloronaphthol Substrate Solution

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.

Cytiva Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used.

Content And Storage | 2°C to 8°C |
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Sensitivity | ∼10 pg |
Product Type | ECL Western Blot Detection Reagent |
For Use With (Application) | Western Blot |
Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Content And Storage | 2°C to 8°C |
---|---|
Sensitivity | ∼1 pg |
Product Type | ECL Western Blot Detection Reagent |
For Use With (Application) | Sensitive Quantitative or Confirmatory Western Blot |
Thermo Scientific™ SuperSignal™ West Pico PLUS and SuperSignal™ West Femto Sample
Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto
Contains SuperSignal™ West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.
Novus Biologicals™ UltraScence Femto Plus Western Substrate
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Luminol-based enhanced chemiluminescent substrate