Western Blot Substrates and Substrate Kits
Western Blot Substrates and Substrate Kits
Thermo Scientific™ SuperSignal™ West Femto and SuperSignal™ West Pico PLUS Sample
Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

Thermo Scientific™ BCIP Substrate Powder (5-bromo-4-chloro-3'-indolylphosphate p-toluidine)
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolylphosphate solutions for chromogenic detection of alkaline phosphatase probes.
Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.
Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution
Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.
Thermo Scientific™ Pierce™ DAB Substrate Kit
Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.
Thermo Scientific™ 1-Step™ NBT/BCIP plus Suppressor Substrate Solution
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.
Thermo Scientific™ SuperSignal™ West Pico PLUS and SuperSignal™ West Femto Sample
Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Atto + trial-size Pico PLUS
Contains SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both routine and high sensitivity western blotting.
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis
Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.
Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto
Contains SuperSignal™ West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.
Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate
SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.
Thermo Scientific™ SuperSignal™ Western Blot Substrate Trial Pack, Atto & Pico PLUS
Contains sample sizes of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate and SuperSignal™ West Pico PLUS Chemiluminescent Substrate to enable trial use of the most recommended substrates for routine and high-sensitivity western blotting.
Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Pico PLUS + trial-size Atto
Contains SuperSignal™ West Pico PLUS Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate, providing chemiluminescent detection substrates for both routine and high sensitivity western blotting.
Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
Thermo Scientific™ 1-Step™ NBT/BCIP Substrate Solution
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.
Thermo Scientific™ Pierce™ CN/DAB Substrate Kit
Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.
Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution
Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.
Thermo Scientific™ NBT Substrate Powder (nitro-blue tetrazolium chloride)
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.
Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers
Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.
Promotions are available
MilliporeSigma™ Calbiochem™ TMB, Insoluble
Peroxidase substrate which produces stable, insoluble, aquamarine blue precipitate at reaction site.
Cytiva Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.
Promotions are available
MilliporeSigma™ Luminata Crescendo ELISA HRP Substrate
Used on immunoblots as chemiluminescent substrate
Promotions are available
MilliporeSigma™ Immobilon Crescendo Western HRP substrate
For use on immunoblots
Promotions are available
MilliporeSigma™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price
Promotions are available
MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns
Promotions are available
Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.
Promotions are available
MilliporeSigma™ Immobilon Classico Western HRP substrate
For use on immunoblots
Promotions are available
MilliporeSigma™ Immobilon Forte Western HRP substrate
For use on immunoblots
Promotions are available