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Western Blot Substrates and Substrate Kits

Western Blot Substrates and Substrate Kits

Chemiluminescent substrates and chromogenic precipitating substrates for western blot detection of alkaline phosphatase (AP) and horseradish peroxidase (HRP) enzyme conjugates; includes both routine and high-sensitivity detection materials.
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1 – 15 of 124 results
1

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
GSA_VA

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

EDGE
Shipping Condition Room Temperature
Substrate Horseradish Peroxidase
Recommended Antibody Concentrations 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
Conjugate HRP
Form Liquid
Product Type Substrate
Signal Duration 6 to 24 hr.
For Use With (Application) Western Blot
Product Line SuperSignal™
Detection Method Chemiluminescence
2

Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate

SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.

EDGE
Shipping Condition Wet Ice
Substrate Horseradish Peroxidase
Sensitivity Low-femtogram to high-attogram
Form Liquid
Product Type Substrate
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal™
Detection Method Chemiluminescence
3

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate
GSA_VA

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

EDGE
Substrate Horseradish Peroxidase
Sensitivity Mid-femtogram
Form Liquid
Product Type Substrate
Signal Duration 24 hr.
Product Line SuperSignal™
Detection Method Chemiluminescence
4

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate
GSA_VA

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

EDGE
Substrate Horseradish Peroxidase
Form Liquid
Product Type Substrate
Signal Duration 6 to 8 hr.
Product Line SuperSignal™
Detection Method Chemiluminescence
5

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate
GSA_VA

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

EDGE
Substrate Horseradish Peroxidase
Content And Storage Upon receipt store at 2-8°C. Product is shipped at ambient temperature.
Form Liquid
Product Type Substrate
Signal Duration 1 to 2 hr.
Product Line Pierce™
Detection Method Chemiluminescence
6

Thermo Scientific™ 1-Step™ NBT/BCIP Substrate Solution

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

EDGE
7

MilliporeSigma™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)
GSA_VA

Provides high sensitivity over a broad detection range at an economical price

EDGE
Substrate Horseradish Peroxidase
Content And Storage 2°C to 8°C
Shelf Life 9 Months
For Use With (Application) Dot/Slot Blot
8

Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate
GSA_VA

Recommended HRP substrate for fluorescence imagers

EDGE
Content And Storage 2°C to 8°C
Kit Contents Detection reagent 1 and 2
Product Type ECL Western Blot Substrate
9

Thermo Scientific™ 1-Step™ Chloronaphthol Substrate Solution

1-Step™ Chloronaphthol Substrate Solution

EDGE
10
Promotions Available

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent
GSA_VA

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.

EDGE
11

Cytiva Amersham™ ECL™ Western Blotting Detection Reagents
GSA_VA

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used.

EDGE
Content And Storage 2°C to 8°C
Sensitivity ∼10 pg
Product Type ECL Western Blot Detection Reagent
For Use With (Application) Western Blot
12
Promotions Available

Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent
GSA_VA GREENER_CHOICE

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

EDGE
Content And Storage 2°C to 8°C
Sensitivity ∼1 pg
Product Type ECL Western Blot Detection Reagent
For Use With (Application) Sensitive Quantitative or Confirmatory Western Blot
13

Thermo Scientific™ SuperSignal™ West Pico PLUS and SuperSignal™ West Femto Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

ENCOMPASS
14

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto

Contains SuperSignal™ West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.

15

Novus Biologicals™ UltraScence Femto Plus Western Substrate
SDP

Luminol-based enhanced chemiluminescent substrate