Electron Microscopy Supplies
Electron Microscopy Sciences EMS Dial A Grid-N-Block Storage With Unique Number
24 slots labeled with letters from A-X where the grids can be stored and rotated for easy access as well as 3 additional cavities for block storage.
Crescent Chemical Co Inc D.E.R. 736 PRACT.
D.E.R. 736 pract. Shorter chain than D.E.R. 732, lower viscosity, gives less flexible blocks. Epoxy equivalent weight 175 - 205; viscosity 0.03 - 0.06 Pas at 25 C.Used in electron microscopy. Size - 100ML Storage Conditions - +15 C TO +30 C
Electron Microscopy Sciences Gilder Finder Grid F1, 300 Mesh, Copper
Used to identify specific areas of mesh by an alphabetical or numerical code built into the mesh. 3.05 mm diameter, 0.7 mil (18 um) thickness. The block of 625 (25x25) individual cells is divided into smaller blocks bounded by thicker crenellated bars. Each block of 25 cells has a central alphabet letter from A to Y for identification purposes.
Grid: Copper
Electron Microscopy Sciences Quantifoil Holey Carbon Film R 6/100 on Gold 300 mesh Grid
QUANTIFOIL is a perforated support foil with pre-defined hole size, shape and arrangement. It has advantages in electron microscopy (EM) or low-energy electron point source (LEEPS) microscopy when compared with conventional holey film.
QUANTIFOIL with circular holes is used in cryo-electron tomographic reconstruction. The roundness of the holes is advantageous with respect to the formation of an ice layer of constant thickness. The whole size chosen depends on the magnification used, and on whether or not one wishes to include support film in the image. Assessment of the image quality is easier when foil is included in the picture, because the power spectrum of a foil is stronger than that of unsupported ice.
Electron Microscopy Sciences Quantifoil Holey Carbon Film R 3/5 on Copper 400 mesh Grid
QUANTIFOIL is a perforated support foil with pre-defined hole size, shape and arrangement. It has advantages in electron microscopy (EM) or low-energy electron point source (LEEPS) microscopy when compared with conventional holey film.
QUANTIFOIL with circular holes is used in cryo-electron tomographic reconstruction. The roundness of the holes is advantageous with respect to the formation of an ice layer of constant thickness. The whole size chosen depends on the magnification used, and on whether or not one wishes to include support film in the image. Assessment of the image quality is easier when foil is included in the picture, because the power spectrum of a foil is stronger than that of unsupported ice.
Electron Microscopy Sciences Lacey Carbon Film with Carbon/Formvar 1 nm / 5 nm thick on 200 mesh Nickel Grid
The continuous ultrathin carbon/formvar film on lacey carbon allows for the thinnest support film that still has adequate strength to withstand electron microscope specimen preparation. The carbon 1 nm, formvar 5 nm film lies across a carbon lacey film supported by a mesh grid. The size of the holes in the lacey film differ widely from batch to batch but are generally in the range of ¼ µm to 5µm, which gives the equivalent support of at least 6000 mesh grid.
Specimen material lying over the covered holes can be imaged in the TEM with practically no interference from the carbon film supporting it. This product is ideal for looking at nanotubes, virus particles and other small particulate material.
Grid: Nickel
Electron Microscopy Sciences Graphene Oxide Support film on Quantifoil R2/2, Cu 300 mesh
Graphene Oxide (GO) support film is a super thin (<1nm), naturally hydrophilic layer placed over the support film on grids. Pre-treatment of GO Support Films is unnecessary - by default, the hydrophilic surface spreads particles evenly across the grid. A hydrophobic surface can be achieved by heating in the air.
It works well with holey carbon, lacey carbon, and Quantifoil grid types, effectively spanning the gaps. It is less expensive than graphene to produce due to complexity of graphene manufacturing and has a better background contrast than graphene resulting in higher resolution. Nearly transparent in electron beam and barely visible under optical microscopes. Regular batch checking ensures correct coverage of monomers.
Note: plasma cleaning or glow discharge will damage the support film.
Material: Copper
Mesh: 300
Electron Microscopy Sciences Graphene Oxide 1 Layer on Thermal SiO2 on Ultra-Flat Silicon
Graphene oxide on ultra-flat thermal SiO2.
Type: 1 layer
Electron Microscopy Sciences Graphene on 2000 Mesh Cu Grid, 1 layer, 0.35 nm thick, 96.4% Transparency
CVD graphene on microporous copper TEM grids with beryllium-copper support aperture. The graphene film appears as a near-transparent to light-grey film on the surface of the red-brown microporous copper TEM grid. For support, the TEM grid is attached using epoxy to a gold-colored beryllium-copper disk with a 2 x 1 mm aperture. Graphene coverage of the TEM grid is better than 75 percent.
Layers: 1 layer
Thickness: 0.35 nm
Transparency: 96.4%
Electron Microscopy Sciences Quantifoil Holey Carbon Film R 2/4 Special Copper, 200 mesh Grid
QUANTIFOIL is a perforated support foil with pre-defined hole size, shape and arrangement. It has advantages in electron microscopy (EM) or low-energy electron point source (LEEPS) microscopy when compared with conventional holey film.
QUANTIFOIL with circular holes is used in cryo-electron tomographic reconstruction. The roundness of the holes is advantageous with respect to the formation of an ice layer of constant thickness. The whole size chosen depends on the magnification used, and on whether or not one wishes to include support film in the image. Assessment of the image quality is easier when foil is included in the picture, because the power spectrum of a foil is stronger than that of unsupported ice.
Grid: Copper
Mesh: 200
Electron Microscopy Sciences EDX-XEDS Calibration TEM Window Grid
Germanium is an ideal calibration standard for x-ray energy dispersive spectroscopy in electron microscopes since it is not typically found in TEM columns it cannot be mistaken for instrument components and their signal peaks. For TEM the Ge is suspended across two micron pores that are patterned on a grid of 20 nm thick silicon nitride. The single 500 x 500 micron window is compatible with high tilt angle tomography, since at 70 degrees of tilt, the thin and beveled 100 micron silicon frame allows you to use a ~50x50 micron region within the center of the window from any rotational orientation.
The specimen can be used for detector energy axis and energy resolution calibration, detector window transmission evaluation, detector solid angle measurements, electron optical instrument system peak measurements, and specimen holder penumbra measurements.
Electron Microscopy Sciences Graphene on Lacey Carbon on 300 Mesh Cu Grid, 3-5 layers, 1.0-1.7 nm thick, 85.8-90.4% Transparency
CVD Graphene TEM support films supported by lacey carbon film on a 300 mesh copper TEM grid. The high quality graphene is produced by chemical vapor deposition, a process by which gaseous reactants are deposited onto a substrate. The graphene film appears as a near-transparent to light-grey film on the surface of the lacey carbon mesh on a red-brown colored copper TEM grid. Graphene coverage of the TEM grid is better than 75 percent.
Layers: 3-5 layers
Thickness: 1.0-1.7 nm
Transparency: 85.8-90.4%