PCR Equipment and Supplies
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Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG
TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Content And Storage | Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
| Fidelity (vs. Taq) | 2 X |
| Purity or Quality Grade | UP (Ultra Pure) |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Product Type | Universal Master Mix II |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| Product Line | TaqMan |
Applied Biosystems™ SYBR™ Green Universal Master Mix
Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Format | Tube |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| Reaction Speed | Standard |
| Label or Dye | SYBR Green |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Product Line | SYBR |
| Detection Method | SYBR |
Applied Biosystems™ TaqMan™ Genotyping Master Mix
Optimized for end-point fluorescence detection in SNP genotyping applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Fidelity (vs. Taq) | 2 X |
| Purity or Quality Grade | UP (Ultra Pure) |
| Concentration | 2X |
| For Use With (Application) | Genotyping |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Genotyping Target | Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known) |
| Product Type | Genotyping Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 |
| Sample Type | DNA (Genomic) |
| Reaction Speed | Standard |
| Technique | SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation) |
| Product Line | TaqMan |
Applied Biosystems™ TaqMan™ Fast Virus 1-Step Multiplex Master Mix for qPCR (No ROX)
TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) is designed to enable dye flexibility for multiplexing up to four different RNA/DNA targets for high-throughput RT-qPCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| InhibitorToleranceSimple | High |
| Concentration | 4X |
| For Use With (Application) | Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 4-plex |
| Detection Method | Primer-probe |
| Thermostability | Not Applicable |
| Passive Reference Dye | None |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System |
| Sample Type | RNA, DNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | RNA only, Both RNA and DNA |
| Hot Start | Built-In Hot Start |
Applied Biosystems™ TaqMan™ Gene Expression Master Mix
Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Product Line | TaqMan |
| Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)
TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Product Type | PreAmp Master Mix |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System |
| Sample Type | cDNA |
| For Use With (Application) | Pre-amplification |
| PCR Method | qPCR |
| Product Line | TaqMan |
| Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ GTXpress™ Master Mix
Combines AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Concentration | 2X |
| For Use With (Application) | Genotyping |
| PCR Method | qPCR |
Applied Biosystems™ TaqMan™ Multiplex Master Mix
Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq Fast DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2 X |
| Product Type | Multiplex Master Mix |
| Sample Type | DNA |
| Reaction Speed | Fast |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Virus Detection |
| PCR Method | Multiplex qPCR |
| Product Line | TaqMan |
| Detection Method | Primer-probe |
Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix, no Rox
TaqPath™ BactoPure™ Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors. Does not contain a passive reference, recommended for use with assays utilizing JUN dye or higher order multiplexing (> three targets).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| InhibitorToleranceSimple | Very High |
|---|---|
| For Use With (Application) | Microbial Detection |
| PCR Method | qPCR |
| Multiplex Capability | Up to 4-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Sensitivity | Down to 1 copy |
| Passive Reference Dye | None |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™ 12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System |
| Sample Type | DNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | cDNA or gDNA only |
| Product Line | TaqPath |
Applied Biosystems™ Fast SYBR™ Green Master Mix
Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast |
| Label or Dye | SYBR Green |
| Product Line | SYBR |
Invitrogen™ SuperScript™ IV Reverse Transcriptase
SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript IV |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Applied Biosystems™ Platinum™ Multiplex PCR Master Mix
The Platinum Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum Taq DNA Polymerase |
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Size (Final Product) | 2.5 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,Multiplex PCR |
| Multiplex Capability | Up to 20-plex |
Invitrogen™ Platinum™ Taq Green Hot Start DNA Polymerase
Invitrogen Platinum Taq Green Hot Start DNA Polymerase provides Platinum Taq DNA Polymerase with a 10X Green PCR Buffer.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Platinum™ II Green PCR Buffer (5X)
Invitrogen 5X Platinum II Green PCR Buffer for fast and easy PCR
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)
Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | Medium |
| Fidelity (vs. Taq) | 1X |
| Format | Tube |
| Size (Final Product) | 5 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start PCR Master Mix |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Overhang | 3'-A |
| Concentration | 2X |
| Starting Material | DNA |
| For Use With (Application) | Hot-start PCR |