PCR Supplies
New England Biolabs, Inc. Luna Universal qPCR Master Mix – 200 rxn (2 x 1 ml)
The NEB Luna Universal qPCR Master Mix is an optimized 2X reaction mix for real-time qPCR detection and quantitation of target DNA sequences using the SYBR/FAM channel of most real-time qPCR instruments. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap with fluorescent dyes used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR, and existing as well as commercial qPCR assay primer sequences can be used.
Meridian Life Science DNTP MASTER MIX 4X500UM/PK
Ready to use molecular-grade dNTP mix containing the four dNTPs (dATP, dCTP, dGTP, dTTP). The mix is designed to save hands-on time for researchers and by reducing the amount of pipetting, makes contamination less likely, 100mM.
Takara Bio ADVANTAGE 2 PCR ENZYME SYSTEM
Advantage 2 PCR Enzyme Kit, 30 rxns, An optimized PCR enzyme mix for use in applications that involve longer templates or require increased fidelity and higher PCR performance
New England Biolabs, Inc. Phusion™ High-Fidelity DNA Polymerase – 500 units
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl2 (1.5 mM at the final [1X] reaction concentration).
New England Biolabs, Inc. NheI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence G^CTAGC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Agilent Technologies 40 RXN PFUULTRA II FUSION HS
PfuUltra II Fusion HS DNA Polymerase; 40 rxn; 1mL; Store at -20 deg.C; Extended length capability up to 19 kb
101 BIO 1-Drop PCR Master Mix (with dye)
User can squeeze one drop of PCR Master mix into PCR tube/plate, add template and primers, and then can run PCR reaction. This PCR Master Mix contains Taq DNA Polymerase, PCR buffer, Mg2+, dNTP, PCR stabilizer and PCR enhancer. This unique Mix recipe makes the system very reliable, and tolerant to most of the PCR condition (concentration of the components). The Taq Polymerase in the PCR Master Mix is Hot Start polymerase, which activity is inhibited at ambient temperatures by the chemical modificaiton. This prevents the formation of misprimed products and primer dimers at ambient temperatures. So no need to prepare the reactions on ice. The Polymerase is activated by a 10 minutes, 95°C incubation.
New England Biolabs, Inc. OneTaq DNA Polymerase – 1000 units
The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template's GC content. OneTaq DNA Polymerase is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10-20% to reactions containing OneTaq GC Reaction Buffer.
Meridian Life Science SensiFAST SYBR No-ROX Kit, 500 x 20 uL Reactions
Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions.
ABclonal Technology 2X Universal SYBR Green Fast qPCR Mix, 25 mL, SYBR Green I, ABclonal Hot Start Taq DNA Polymerase, Universal ROX Dye
The ABclonal 2X Universal SYBR Green Fast qPCR Mix is a highly efficient solution tailored for sensitive real-time quantitative PCR applications. This ready-to-use mix combines advanced components including a hot start polymerase, optimized buffers, and a proprietary dye, ensuring accurate results across a variety of qPCR instruments. Its formulation minimizes non-specific amplification and enhances specificity across diverse samples.
- 2X concentrated format
- Compatible with all qPCR instruments
- Hot start capability for improved specificity
- Includes SYBR Green I and universal ROX dye
- Demonstrates high reaction efficiency (99%)
- Features excellent freeze-thaw stability
- Recommended for target lengths of 70-200 bp
- Storage temperature of -20℃
- Used for research purposes only
- Protect from light during storage
Takara Bio PRIMESTAR MAX DNA POLY 100RXNS
PrimeSTAR Max DNA Polymerase Ver.2 is a high-performance PCR amplification system that offers the highest levels of fidelity and extension speed. With an improved success rate of target amplification in high-speed reactions compared to PrimeSTAR Max DNA Polymerase (Cat. # R045A/B), PrimeSTAR Max DNA Polymerase Ver.2 maintains the fidelity, making it a fully compatible and superior alternative. As a 2X premixed reagent with an antibody-mediated hot start formulation to suppress DNA Polymerase activity and 3′→5′ exonuclease activity, it allows quick preparation of reactions at room temperature and is useful for high-throughput applications.Reaction size: 1 rxn equals a 50 µl total reaction volume.
New England Biolabs, Inc. Q5® Hot Start High-Fidelity DNA Polymerase – 500 units
Q5 Hot Start High-Fidelity DNA Polymerase is a high-fidelity, thermostable, hot start DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 Hot Start High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (>= 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 Hot Start High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
Agilent Technologies D1000 Sample Buffer
For the analysis of DNA from 35 to 1000 bp. Sample buffer only.