PCR Supplies
New England Biolabs, Inc. ProtoScript II Reverse Transcriptase – 10000 units
ProtoScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48C, providing higher specificity, higher yield of cDNA and more full-length cDNA product up to 12 kb.
Viagen Biotech Inc PROTEINASE K SOLUTION
Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a serine protease. The smallest peptide to be hydrolysed is a tetrapeptide. DNA Extraction lysis reagent for DNA tests & genotyping: The Proteinase K solution is useful when a small amount of biological samples are processed. Add the enzyme solution to DirectPCR reagents at the final concentration of 0.5-1.0 mg/ml (25-50 ul Proteinase K solution per 1 ml DirectPCR reagent). Follow the instruction manuals for DirectPCR Reagent Protocol/Manual for each blood, blood stain, buccal swab, hair root DNA genotyping. Once diluted in DirectPCR reagents, Proteinase K is stable for ~24 hrs.
Clontech Labs 3P PrimeSTAR® GXL DNA Polymerase, 1,000 Units
A hot-start, high-fidelity PCR enzyme, PrimeSTAR GXL DNA Polymerase excels in reactions with GC-rich templates, excess template, and long amplicons up to 30 kb (GXL). The polymerase is supplied with separate tubes of optimized buffer (Mg2+ plus), and dNTPs. 1,000 Units
SCIENCELL RESEARCH LABORATORIES INC TELOMERASE ACTIVITY QUANT KIT
NC2113748 TELOMERASE ACTIVITY QUANT KIT
BIOSEARCH TECHNOLOGIES INC (Lucigen) FailSafe PCR System, 250 U @2.5 U/LPCR System kit for end-point applications. Achieve consistent, high-fidelity results for every DNA template.
The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe PCR System will faithfully amplify your template every time.We promise!
Sigma Aldrich Fine Chemicals Biosciences JumpStart REDTaq ReadyMix Reaction Mix | MFCD00166026 | 100 rxns
JumpStart REDTaq ReadyMix Reaction Mix | MFCD00166026 | 100 rxns
Clontech Labs 3P Adeno-X™ Rapid Titer Kit, 120 Titrations
The Adeno-X Rapid Titer Kit takes advantage of production of viral hexon proteins for the titration of adenoviral stocks in 48 hours. A viral stock is tested by making serial dilutions, infecting HEK 293 cells, and 48 hours later, fixing and staining those cells with the Mouse Anti-Hexon Antibody. The signal is detected with a Rat Anti-Mouse Antibody conjugated to horseradish peroxidase (HRP) and developed with metal-enhanced 3,3'- Diaminobenzidine tetrahydrochloride (DAB). 120 Titrations
New England Biolabs, Inc. Luna Universal qPCR Master Mix – 200 rxn (2 x 1 ml)
The NEB Luna Universal qPCR Master Mix is an optimized 2X reaction mix for real-time qPCR detection and quantitation of target DNA sequences using the SYBR/FAM channel of most real-time qPCR instruments. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap with fluorescent dyes used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR, and existing as well as commercial qPCR assay primer sequences can be used.
Agilent Technologies PFU TURBO AD 100 UNITS
PfuTurbo DNA polymerase AD features a reformulated buffer system for increased economy with the same high performance as the original PfuTurbo DNA polymerase for robust, high-fidelity PCR.
New England Biolabs, Inc. WarmStart RTx Reverse Transcriptase – 250 reactions
WarmStart RTx Reverse Transcriptase is a unique in silico designed RNA-directed DNA polymerase coupled with a reversibly-bound aptamer that inhibits RTx activity below 40C. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template. RTx is a robust enzyme for RNA detection in amplification reactions and is particularly well suited for use in LAMP (loop-mediated isothermal amplification). The WarmStart property enables high throughput applications, room temperature setup, and increases the consistency and specificity of amplification reactions. RTx contains intact RNase H activity.