Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Applied Biosystems™ resDNASEQ™ dPCR HEK293 DNA Kit
The Applied Biosystems resDNASEQ dPCR HEK293 DNA Kit is a digital PCR (dPCR)-based solution to quantitate residual DNA from HEK293 expression systems, which are commonly used for production of gene therapies, cell-based vaccines, and similar therapeutics.
Applied Biosystems™ TrueMark™ PGx Comprehensive Plasmid Controls
TrueMark PGx Comprehensive Plasmid Controls contains three linearized multi-target plasmid pools with human genome target sequences commonly used for pharmacogenomic research testing.
Applied Biosystems™ TaqMan™ QSY and QSY2 Probes
Applied Biosystems TaqMan QSY and QSY2 probes are dual-labeled probes used for real-time PCR. TaqMan QSY and QSY2 Probes are designed for optimal sensitivity and performance in multiplexed qPCR experiments for detection of up to 6 targets.
Applied Biosystems™ resDNASEQ™ Quantitative E1A DNA Fragment Length Kits
The resDNASEQ Quantitative E1A DNA Fragment Length Kit is a quantitative PCR (qPCR)-based system that measures and quantitates the fragment lengths of residual host-cell DNA by targeting the E1A gene in HEK 293 cell lines.
Applied Biosystems™ ViralSEQ™ Quantitative Sf-Rhabdovirus Kits
The Applied Biosystems ViralSEQ Quantitative Sf-Rhabdovirus Kit is a two-step RT-qPCR assay for the detection of Sf-rhabdovirus in recombinant products manufactured in insect cell lines using baculovirus expression systems.
Applied Biosystems™ TaqMan™ SARS-CoV-2, Flu A/B, RSV RNA Control
The TaqMan SARS-CoV-2, Flu A/B, RSV RNA Control contains targets specific to the SARS-CoV-2, influenza A/B, and RSV genomic regions targeted by the TaqMan SARS-CoV-2, FluA/B RSV RT-PCR Assay Kit.
Applied Biosystems™ TaqMan™ MicroRNA Reverse Transcription Kit
Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.
Applied Biosystems™ resDNASEQ™ Quantitative MDCK DNA Kits
The resDNASEQ™ Quantitative MDCK DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines.
Applied Biosystems™ resDNASEQ™ Quantitative Pichia pastoris DNA Kits
The resDNASEQ™ Quantitative Pichia pastoris DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Pichia pastoris cells.
Applied Biosystems™ MeltDoctor™ HRM Positive Control Kit
Provides nucleic acid controls to demonstrate and troubleshoot High Resolution Melt (HRM) analysis
Invitrogen™ ROX Reference Dye
ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.
Applied Biosystems™ PureQuant B Cell Assay
The Applied Biosystems PureQuant assays are a family of assays that accurately identify and quantify specific immune cell types in a mixed population of cells. These assays are TaqMan qPCR-based and measure genomic DNA methylation at specific sites that serve as a unique identifier. The assays include standards and controls and each 96-well plate can accommodate up to seven test samples.
MP Biomedicals™ RapidScript™ RT Kit
Use the RapidScript™ RT Kit to cDNA synthesize from 15 kb RNA templates in 10 to 15 min.
VetMAX™ PEDV/TGEV/SDCoV Kit
A single-well multiplex real-time RT-PCR kit for the detection and differentiation of the porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and swine delta corona virus (SDCoV) in environmental samples.
Thermo Scientific™ SureTect ™ Vibrio cholerae, V parahaemolyticus and V. vulnificus PCR Assay
Rapidly detect Vibrio cholerae, V. parahaemolyticus and V. vulnificus in food samples in as little as 10 hours with this highly-specific, simple-to-use real-time PCR Assay.