Additional Selective and Differential Cell Culture Media
NEOGEN Brain Heart Infusion Agar, 500 g
Brain Heart Infusion Agar is used for the cultivation of a wide variety of fastidious organisms and is not intended for use in the diagnosis of disease or other conditions in humans.A general purpose nutritious agar base. This medium was first used for the isolation of dental pathogens. With the addition of 7% defibrinated blood the medium will support the growth of a wide range of fastidious organisms, the phosphate buffer will help neutralize the acids produced from the utilization of glucose and thus maintain viability. The medium is not recommended for the determination of hemolytic reactions because of the glucose content.
For more information visit https://www.neogen.com/solutions/Microbiology/brain-heart-infusion-agar
NEOGEN Tryptic Soy Broth (Soybean-Casein Digest Broth), 5 kg
Tryptic Soy Broth (Soybean-Casein Digest Broth) is used for the cultivation of a wide variety of microorganisms. Tryptic Soy Broth conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans.Tryptic Soy Broth, a general purpose medium, is commonly referred to as Casein Soya Bean or Soybean-Casein Digest broth, and abbreviated as TSB. This medium was originally developed for use without blood in determining the effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry.For more information visit https://www.neogen.com/solutions/Microbiology/tryptic-soy-broth
NEOGEN Brilliant Green Agar, 500 g
Brilliant Green Agar is used for the selective isolation of Salmonella spp. in a laboratory setting. Brilliant Green Agar is not intended for use in the diagnosis of disease or other conditions in humans.Kristensen, Lester, and Jurgens first described the use of Brilliant Green Agar as a primary plating medium for the isolation of Salmonella. The report described the medium as useful for the differentiation of “paratyphoid B” from other intestinal gram-negative bacilli. Kaufmann modified their formula and used Brilliant Green Agar in addition to Tetrathionate Broth for the isolation of Salmonella from stool specimens. The outstanding selectivity of this medium permits use of moderately heavy inocula, which should be evenly distributed over the surface. Brilliant Green Agar is used in the microbial limits test. Brilliant Green Agar supplemented with novobiocin is used in food testing.For more information visit https://www.neogen.com/solutions/Microbiology/brilliant-green-agar-ncm0283
NEOGEN Cronobacter Selective Broth, 500 g
Cronobacter Selective Broth (CSB) is recommended by ISO 22964 for the isolation and identification of Cronobacter spp. (formerly Enterobacter sakazakii), and is not intended for use in the diagnosis of disease or other conditions in humans.Although rarely causing infections in immunocompetent adults, members of the Cronobacter genus have been implicated in sepsis, meningitis and necrotizing enterocolitis with a high death rate in neonates. These opportunistic pathogens are common in the environment and their ability to survive desiccation presents a significant risk for post-pasteurization contamination and survival in spray dried milk products. Enzymatic digest of animal tissues and meat extract provide a source of nitrogen and essential vitamins.For more information visit https://www.neogen.com/solutions/Microbiology/cronobacter-selective-broth
NEOGEN m-Enterococcus Agar, 500 g
m-Enterococcus Agar is used for the selective isolation and enumeration of enterococci by membrane filtration in a laboratory setting. m-Enterococcus Agar is not intended for use in the diagnosis of disease or other conditions in humans.m-Enterococcus Agar was first described by Slanetz et al. for the enumeration of enterococci by the membrane filtration technique. In 1957, Slanetz and Bartley modified this medium by adding triphenyl tetrazolium chloride (TTC). Increased recovery and larger colonies were obtained by incubating the inoculated membranes on the agar surface instead of on pads saturated with liquid medium. The membrane filtration method is simple to perform, does not require confirmation, and permits a direct count of enterococci in 48 hours. m-Enterococcus Agar is also referred to as m-Azide Agar.For more information visit https://www.neogen.com/solutions/Microbiology/m-enterococcus-agar
NEOGEN MacConkey Broth Purple, 500 g
MacConkey Broth Purple is used for the detection and enumeration of fecal coliforms (37°C) and E. coli (44°C). This medium is not intended for use in the diagnosis of disease or other conditions in humans.The replacement of neutral red used in the original formulation by bromocresol purple makes the color change caused by acid producing organisms easier to read.For more information visit https://www.neogen.com/solutions/Microbiology/macconkey-broth-purple
NEOGEN Tryptic Soy Blood Agar Base No. 2, 500g
Tryptic Soy Blood Agar Base No. 2 is used with blood for the isolation and cultivation of a wide variety of microorganisms in a laboratory setting. Tryptic Soy Blood Agar Base No. 2 is not intended for use in the diagnosis of disease or other conditions in humans.Blood Agar Bases are typically supplemented with 5 - 10% sheep, rabbit, or horse blood, and used for isolating, cultivating, and determining hemolytic reactions of fastidious pathogenic microorganisms. Without enrichment, this blood agar base can be used as a general purpose medium.Tryptic Soy Agar is based on the Soybean-Casein Digest formula specified by US Pharmacopeia. Tryptic Soy Agar is a nutritious base, and a variety of supplements, including blood, are often added to enhance this medium.For more information visit https://www.neogen.com/solutions/tryptic-soy-blood-agar-base-number-2
NEOGEN Agar, Bacteriological No. 1, 500 g
Agar, Bacteriological No. 1 is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Bacteriological No. 1 is not intended for use in the diagnosis of disease or other conditions in humans. Agar, Bacteriological No. 1 conforms with the testing specified in the USP/EP Pharmacopeia.Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperature for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.For more information visit https://www.neogen.com/solutions/Microbiology/agar-bacteriological-number-1
NEOGEN Phosphate Buffer, pH 7.2, 500 g
Phosphate Buffer, pH 7.2 is used for the preparation of microbiological dilution blanks in a laboratory setting. Phosphate Buffer, pH 7.2 is not intended for use in the diagnosis of disease or other conditions in humans. The formula for phosphate buffer is specified by the American Public Health Association (APHA) for use in diluting test samples. Phosphate Buffer, pH 7.2 is specified for use in diluting water, dairy products and food for microbiological methods. In the examination of water and dairy products the addition of magnesium chloride is recommended. This buffer is also referred to as Butterfield’s Buffered Phosphate Diluent and recommended for examination of food. Phosphate Buffer, pH 7.2 stabilizes the pH of water used for dilutions.For more information visit https://www.neogen.com/solutions/Microbiology/phosphate-buffer-ph-72
NEOGEN Phenylethanol Agar, 500 g
Phenylethanol Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Phenylethanol Agar is not intended for use in the diagnosis of disease or other conditions in humans. Brewer and Lilley reported the addition of phenylethanol to a nutritive medium permitted growth of Gram-positive organisms, but markedly to completely inhibited growth of Gram-negative organisms. Phenylethanol Agar inhibits swarming of Proteus spp., and can be used to selectively isolate anaerobic bacteria from specimens with mixed flora. Phenylethanol Agar is specified for use in several reference methods.For more information visit https://www.neogen.com/solutions/Microbiology/phenylethanol-agar
NEOGEN Sorbitol MacConkey Agar, 5 kg
Sorbitol MacConkey Agar is used for the isolation of pathogenic Escherichia coli in a laboratory setting. Sorbitol MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans. Sorbitol MacConkey Agar is based on the formula by Rappaport and Henig. Originally developed for isolating enteropathogenic (EPEC) serotypes, O11 and O55, this medium is recommended for the isolation and differentiation of enterohemorrhagic E. coli O157:H7. Serotype O157 has been implicated in serious foodborne diseases. MacConkey Agar W/ Sorbitol contains sorbitol instead of lactose for differentiating enteropathogenic E. coli serotypes; these strains are typically sorbitol negative. MacConkey Agar W/ Sorbitol is recommended for food testing.For more information visit https://www.neogen.com/solutions/Microbiology/sorbitol-macconkey-agar
NEOGEN Bacteriological Peptone, 500 g
Bacteriological Peptone is for use in preparing microbiological culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans.An economical source of nutrients provided by a balanced mixture of meat peptones and tryptone. The growth requirements of most non-fastidious organisms will be fulfilled by the range of amino acids, peptides and proteoses in this mixture.For more information visit https://www.neogen.com/solutions/Microbiology/bacteriological-peptone
NEOGEN Pancreatic Digest of Gelatin, 500 g
Pancreatic Digest of Gelatin is an enzymatic digest of gelatin for use in preparing microbiological culture media in a laboratory setting. Pancreatic Digest of Gelatin is not intended for use in the diagnosis of disease or other conditions in humans.Pancreatic Digest of Gelatin provides a complex nitrogen source and is used as a media ingredient for fermentation studies. Pancreatic Digest of Gelatin alone is used to support the growth of non-fastidious microorganisms. Pancreatic Digest of Gelatin is deficient in carbohydrates and distinguished by low cystine and tryptophan content.For more information visit https://www.neogen.com/solutions/Microbiology/pancreatic-digest-gelatin
NEOGEN Tryptone Glucose Extract (TGE) Agar, 500 g
Tryptone Glucose Extract (TGE) Agar is used for the cultivation and enumeration of microorganisms in dairy and bottled water products. Tryptone Glucose Extract (TGE) Agar is not intended for use in the diagnosis of disease or other conditions in humans. In the 1930’s, Bower and Hucker developed a medium for detecting bacteria in milk and other dairy products. Prickett used a glucose agar containing tryptone to study thermophilic bacteria in milk. This medium is known as Yeast Dextrose Agar. In 1948, the American Public Health Association (APHA) adopted Tryptone Glucose Extract Agar for use in testing milk and dairy products. Currently, APHA specifies Tryptone Glucose Extract Agar for the heterotrophic plate count procedure in testing bottled water. Tryptone Glucose Extract Agar is also known as Yeast Dextrose Agar.For more information visit https://www.neogen.com/solutions/Microbiology/tryptone-glucose-extract-tge-agar
NEOGEN DNase Agar, 500 g
DNase Agar is used for the differentiation of microorganisms on the basis of deoxyribonuclease activity and is not intended for use in the diagnosis of disease or other conditions in humans.In 1956, Weckman and Catlin showed a correlation between increased DNase activity of Staphylococcus aureus and positive coagulase activity. Their research suggested DNase activity could be used to identify potentially pathogenic staphylococci. DiSalvo confirmed their results by obtaining excellent correlation between coagulase and DNase activity of staphylococci isolated from clinical specimens. Jeffries, Holtman, and Guse incorporated DNA in agar to study DNase production by bacteria and fungi. Polymerized DNA precipitates in the presence of 1N HCl, creating an opaque medium. Organisms that degrade DNA produce a clear zone around an inoculum streak.For more information visit https://www.neogen.com/solutions/Microbiology/dnase-agar