General Cell Lines and Cell Cultures
STEMCELL Technologies Human Peripheral Blood Mononuclear Cells, Frozen
Streamline your assays with ready-to-use, ethically sourced, primary human mononuclear cells. With personalized service, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications, we help you get the cells you need. Isolated from peripheral blood leukapheresis samples using density gradient separation and/or red blood cell lysis and cryopreserved in animal component-free CryoStorCS10 medium (Catalog #07930), cells are collected using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added during collection as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.Certain products are only available in select territories.
iQ Biosciences Human PBMCs, 50M cells/vial
High-quality, high-viability (> 90%) peripheral blood mononuclear cells (PBMCs) from healthy blood donors sourced from a world-renowned blood center. Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications. All orders come with an iQ Certificate of Analysis.The following donor requests can be accommodated if inventory is available: - Single or multiple donors for a particular product (SKU). Indicate the number of vials from each individual donor - Positive or negative for EBV or CMV (for healthy donors only) - Good responder or non-responder to tetanus antibody (for healthy donors only) - HLA types for Class I and Class II alleles (for healthy donors only) - Donor(s) with a higher percentage of the cells of interest (e.g., B cells, CD4+ cells, CD8+ cells, NK cells, etc.) - Gender, age, body mass index (BMI), or ethnicity
ADDEXBIO TECHNOLOGIES INS-1 Cell Line, Catalog Number C0018007, Insulinoma, Derived from Rattus Norvegicus Pancreatic Islets, 5% CO2, 37°C, Biosafety Level I
INS-1 Cell Line, Catalog Number C0018007, Insulinoma, Derived from Rattus Norvegicus Pancreatic Islets, 5% CO2, 37°C, Biosafety Level I
Lonza Walkersville NHDF-Ad - Human Dermal Fibroblasts, Adult
Human Dermal Fibroblasts are available from either adult skin (NHDF-Ad) or neonatal foreskin (NHDF-Neo). They are derived from the dermis of normal human neonatal foreskin or adult skin. They are cryopreserved at the end of primary culture. Normal Human Epidermal Keratinocytes (00192627) from matched donors are available for certain lots.Lonza's human dermal fibroblasts are guaranteed through 15 population doublings when using FGM-2 Growth Media. Dermal fibroblasts are characterized by morphological observation throughout serial passage.All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.Applications:ECM protein analysisWound healingCollagen metabolismCosmeticsSkin therapy/models Content:1 x Cryopreserved ampule of NHDF-Ad containing ≥ 500,000 cells
iQ Biosciences Cynomolgus Monkey PBMCs, No Immunophenotyping, 10M cells/vial
High-quality, high-viability (> 90%) cynomolgus (cyno) monkey (or macaque) peripheral blood mononuclear cells (PBMCs) sourced responsibly from a vetted third party facility. Used for a wide variety of safety assessment and functional assays. All orders come with an iQ Certificate of Analysis.The following donor requests can be accommodated if inventory is available: - Single or multiple donors for a particular product (SKU). Indicate number of vials from each individual donor - Donor(s) with a higher percentage of the cells of interest, i.e., B cells, CD14+ cells, CD4+ cells, CD8+ cells, or NK cells
Lonza Walkersville Human Mesenchymal Stem Cell (hMSC) Osteogenic Differentiation Medium BulletKit
Stem cells possess the unique ability to differentiate into multiple lineages of cells including osteoblasts. Lonza’s hMSC Osteogenic Differentiation BulletKit Medium is a serum containing medium designed to induce osteogenic differentiation of human bone marrow derived mesenchymal stem cells (hMSC) into mature, functionally active osteoblasts in as few as 14 days. Differentiation can then be validated by a variety of methods including the use of Lonza’s OsteoImage Bone Mineralization Reagent which provides a quantitative value related to the amount of osteogenic differentiation.Content:1 x hMSC Osteogenic Differentiation Basal Medium (PT-3924), 170 mL1 x hMSC Osteogenic Differentiation SingleQuots Supplements Kit (PT-4120)
Brainxell Inc Cortical Glutamatergic Neurons (Human iPSC-Derived, Male Line, 1M)
Cortical glutamatergic neurons derived from iPSC line WC-30, male. Contents: 1 vial of ≥ 1x10^6 viable cryopreserved cells. Marker Expression: Cortical Glutamatergic Neurons (BX-0300) have high neuronal purity (>80%) and comprise predominantly excitatory neurons. Labeling with the pan-neuronal marker MAP2 (red) highlights the neuronal purity of these cells. Labeling with the glutamatergic-specific marker FOXP2 indicates the overwhelming presence of glutamatergic neurons. Function: Cortical Glutamatergic Neurons exhibit pronounced electrophysiological activity after two weeks in culture, as demonstrated by multi-electrode array (MEA) recordings. Related diseases: Alzheimer's & Huntington's Disease. Primary neurotransmitter released: Glutamate
Leinco Technologies Inc 1X ClearFlow™ Sheath Fluid | 20L
ClearFlow™ Sheath Fluid is specifically formulated isotonic solution for transporting particles through a flow cytometer and supplied "Ready to Use". ClearFlow™ is specially manufactured in our ISO 7(class 10,000) cleanroom suite for low particulate count (SEE ABOVE EXPERIMENT) and fluorescence background to guarantee better signal to noise ratio measurements.
Lonza Walkersville P4 Primary Cell 4D-Nucleofector X Kit S
The P4 Primary Cell 4D-Nucleofector X Kit S is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsMedium throughput applicationsOptimization of transfection conditions e.g. substrate titrationsContent:1 x 0.675 mL P4 Primary Cell Nucleofector Solution1 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)2 x 16-well Nucleocuvette Strips (20 μl)
Invivogen InvivoGen HEK-Blue™ IL-7 Reporter Cells, Detects Bioactive IL-7, Monitors JAK/STAT5 Pathway, 3 Vials
The InvivoGen HEK-Blue™ IL-7 Reporter Cells are engineered HEK 293 cells designed for detecting and quantifying interleukin-7 (IL-7) through the activation of the JAK/STAT5 signaling pathway. This product offers benefits in screening antibodies and small molecule inhibitors targeting IL-7, ensuring high responsiveness to both human and murine IL-7, setting it apart from standard cell lines.
Lonza Walkersville Rat Neuron Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works with cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary rat neurons.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Applications - Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Rat Neurons0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Associates of Cape Cod Inc CONTROL STANDARD ENDOTOXIN 5PK
CONTROL STANDARD ENDOTOXIN (CSE) FOR PYROCHROME KITS, 10ng/VIAL, 5 VIALS/PACK, reconstitute with LRW ONLY