General Cell Lines and Cell Cultures
ACROBiosystems HEK293 Cell Line, Cryopreserved, 2 vials (1 mL, ≥5x10^6 cells/vial)
Cryopreserved HEK293 adherent human cell line suitable for transfection and recombinant protein expression. Supplied as two cryovials; each vial contains at least 5x10^6 cells in 1 mL of serum-free cryopreservation medium. Mycoplasma and sterility testing are negative. Store in liquid nitrogen and culture in DMEM supplemented with 10% FBS.
- Cryopreserved, two vials (1 mL each)
- At least 5x10^6 cells per vial
- Adherent culture type
- Store in liquid nitrogen
- Complete growth medium: DMEM + 10% FBS
- Freeze medium: Serum-free cryopreservation medium
- Mycoplasma testing: Negative
- Sterility testing: Negative
iQ Biosciences C57BL/6 Mouse PBMCs, 5M cells/vial
High-quality, high-viability (> 80%) peripheral blood mononuclear cells (PBMCs) from the C57BL/6 mouse, the most commonly-used inbred mouse strain. Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications. All orders come with an iQ Certificate of Analysis.
Lonza Walkersville HSMM – Human Skeletal Muscle Myoblasts
Clonetics Human Skeletal Muscle Myoblasts (HSMM) are isolated from normal donors. HSMMs are isolated from the upper arm or leg muscle tissue and are sold in second passage. HSMMs can be differentiated to form myotubes in culture. Clonetics HSMMs are guaranteed through 10 population doublings and to differentiate to form multinucleated myotubes in culture. All cell types test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.Applications: Cellular developmentDifferentiationDiabetesInsulin uptake or resistanceTissue repairDrug developmentMyotube developmentCo-culture with other cell typesContent:1 x Cryopreserved Ampule with ≥500,000 cells/vial
BPS Bioscience Inc FCRN IgG Recycling HMEC-1 Cell Pool, Cell Pool, HMEC-1, 2 vials, >1 x 10^6 cells per vial, 1 ml of 7.5% DMSO and 92.5% FBS, Research Use Only
FCRN IgG Recycling HMEC-1 Cell Pool, Cell Pool, HMEC-1, 2 vials, >1 x 10^6 cells per vial, 1 ml of 7.5% DMSO and 92.5% FBS, Research Use Only
STEMCELL Technologies Human Peripheral Blood B Cells, Frozen, Size: 1 x 10^7 cells
Human primary B cells derived from peripheral blood (PB) are isolated from mononuclear cells (MNCs) using the negative immunomagnetic selection technique. Cells are untouched and ready for downstream applications. PB was collected using acid-citrate-dextrose solution A (ACDA) as the anticoagulant.Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.Browse our Frequently Asked Questions (FAQs) (https://www.stemcell.com/primary-cells-faq) on Primary Cells.
LGC GENOMICS LLC (Lucigen) CopyCutter EPI400 Electrocompetent E. coli, 10 RxnsClone unstable and toxic sequences in standard plasmids, with high efficiency.
CopyCutter™ EPI400™ E. coli cells will significantly lower the copy number of a wide variety of common vectors to enable cloning of unstable DNA sequences and those expressing toxic proteins
CROSSTEX INTERNATIONAL BACILLUS ATROPHAEUS 10ML
GP Sus-B. atrophaeus Growth Promotion Test Suspensions (QC Organisms)
LGC GENOMICS LLC (Lucigen) OverExpress C43(DE3) Chem Comp Cells, 12 Rxns (SOLOs)
Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Some strains also available in electrocompetent format.Lucigens OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3).
LGC GENOMICS LLC (Lucigen) MC1061 F- Electrocompetent Cells, 24 Rxns (DUOs)High efficiency F-control strain for phage display applications.
Perfect for antibody phage display or peptide phage display library creationThe highest efficiency TG1 competent cells availableYour only source for electrocompetent SS320 and ER2738 cellsAmber suppressor and non-amber suppressor lines for maximum flexibilityHigh efficiency MC1061 F- cells for cloning or control reactionsCreate larger libraries; speed discoveryBulk custom dispensing available with all of our phage display cellsCompetent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
Lonza Walkersville Human CD34+ Cell Nucleofector Kit
The Nucleofector 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human hematopoietic stem cells (CD34+) from cord blood or bone marrow.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human CD34+ Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
System Biosciences LLC MDA-MB-231 BREAST CANCER CELLS
MDA-MB-231 breast cancer cells(Exosomes from). An aggressive,invasive,metastatichuman breast cancer cell line