Cell Based Assays
Biolegend Alexa FluorR 647 anti-AMPKalph
Alexa Fluor 647 anti-AMPKα Phospho (Thr172) [A20017A]; Isotype: Mouse IgG1, κ; Reactivity: Human, Mouse; Apps: IHC-P, ICFC; Size: 100 μg
Meso Scale Discovery U-PLEX Human A2M ASY 25 PL
The Meso Scale Discovery U-PLEX Human A2M Assay is a multiplex immunoassay kit that enables simultaneous quantitative measurement of Alpha-2-Macroglobulin (A2M) in human serum plasma and cell culture supernatants This electrochemiluminescent assay utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and accurate detection of A2M levels down to sub-pg/mL concentrations The assay requires 50 L sample volume and can be read on MSD instrument platforms This product provides a simple rapid way to quantify A2M levels which can aid research into inflammatory conditions immune function and proteolytic regulation
Meso Scale Discovery R-PLEX Human IL-36 ASY
The Meso Scale Discovery R-PLEX Human IL-36gamma Assay is a multiplex immunoassay panel that allows simultaneous quantitative measurement of IL-36gamma in human serum plasma and cell culture supernatants It utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements This assay kit includes a plate coated with capture antibodies detection antibodies labeled with an ECL compound and calibrator and control solutions for generating standard curves and evaluating run validity It allows sensitive reproducible quantification of IL-36gamma levels from human samples
Meso Scale Discovery SPLEX HUM CTLA4 Kit 25 PL
The Meso Scale Discovery CTLA-4 Discovery Kit is a highly sensitive immunoassay that quantitatively measures soluble CTLA-4 in serum plasma and cell culture supernatants The assay uses MSD s electrochemiluminescent detection technology and provides a lower limit of detection of 0 05 ng/mL The kit includes a 96-well plate coated with capture antibody a detection antibody conjugated to an electrochemiluminescent label and calibrators and controls to generate a standard curve The assay takes approximately 3 5 hours to complete and requires a MSD instrument such as SECTOR Imager QuickPlex or Discovery Workbench to read the plate The kit can test up to 80 samples in duplicate
Meso Scale Discovery R-PLEX Human ERbB2 ASY
The R-PLEX Human ERBB2 Assay is a multiplex immunoassay panel that measures ERBB2 protein levels in human serum plasma and cell culture supernatants This assay uses electrochemiluminescence detection technology and is compatible with the SECTOR S 600 instrument The panel allows for the simultaneous quantitative measurement of ERBB2 along with other biomarkers This assay has high sensitivity broad dynamic range and requires low sample volume The R-PLEX Human ERBB2 Assay provides a reproducible and robust tool for cancer research and drug development applications
Revvity Health Sciences Inc HTRF Human Total RIPK1 Detection Kit, 500 Assay Points
HTRF Human Total RIPK1 Detection Kit, 500 Assay Points
Eurofins Discoverx PathHunter eXpress GPR173 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0380E2ACP1M)
The PathHunter GPR173 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR173 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR173 and EA-tagged β-Arrestin-2. Activation of GPR173 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CCR7 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0070E2CP2M)
The cAMP Hunter CCR7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19) based activation of CCR7 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CCR7, a naturally Gi/Go coupled GPCR. Upon activation of CCR7, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL2RB/IL2RG/IL2RA Dimerization Assay, 10pl Kit. (93-1003E3CP0L)
The PathHunter IL2RB/IL2RG/IL2RA Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-2) induced dimerization of IL2RB/IL2RG/IL2RA receptors. This product uses EFC technology. The cell line included overexpresses IL2RB target protein fused to PK alongside IL2RG target protein fused to EA and untagged ILR2A. Upon ligand-induced dimerization of IL2RB/IL2RG/IL2RA, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Tocilizumab Bioassay Kit, 2-Plate. (93-1045B3-00109)
The PathHunter Tocilizumab Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-6) induced dimerization of IL-6ST and IL-6R. receptors. This product uses EFC technology. The cell line included overexpresses IL-6ST target protein fused to PK alongside IL-6R target protein fused to EA. Upon ligand-induced dimerization of IL-6ST and IL-6R, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress GPR135 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0431E2ACP1M)
The PathHunter GPR135 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR135 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR135 and EA-tagged β-Arrestin-2. Activation of GPR135 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR176 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0382E2ACP1L)
The PathHunter GPR176 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR176 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR176 and EA-tagged β-Arrestin-2. Activation of GPR176 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OXTR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0261E2CP2M)
The PathHunter OXTR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. oxytocin) based activation of OXTR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OXTR and EA-tagged β-Arrestin-2. Activation of OXTR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rPROKR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0544PE2RCP0M)
The PathHunter rPROKR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prokineticin) based activation of rPROKR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rPROKR1 and EA-tagged β-Arrestin-2. Activation of rPROKR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 36 Reagent, 500. (93-0563R36B)
AssayComplete Cell Plating 36 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.