Cell Based Assays
Eurofins Discoverx PathHunter Human GPR150 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR150 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR150 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx SPRINTer™ Human c-Myc Targeted Protein Degradation Cell-Based Assay Kit (HCT-116)
The SPRINTer™ Human c-Myc Targeted Protein Degradation Stable Cell Line Assay (HCT-116) contains a stable clonal cell line used for monitoring drug-induced protein turnover of c-Myc, supporting TPD studies. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human THR beta Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1)
The PathHunter Human THR beta Nuclear Receptor Stable Cell Line Protein Interaction Assay (CHO-K1) contains a stable clonal cell line used to measure activated THRβ nuclear hormone receptor interaction with SRCPs. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress ADCYAP1R1 Total Internalization Assay, 10-plate Kit. (93-0885E3CP0L)
The PathHunter ADCYAP1R1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of ADCYAP1R1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged ADCYAP1R1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the ADCYAP1R1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Cell Migration/Chemotaxis Assay Kit (96-well, 3 ¿m)
Cell Migration/Chemotaxis Assay Kit (96-well, 3 ¿m)
The product is subject to the following: Abcam Restricted Use Statement
Bioassay Systems QuantiChrom™ Hydroxyproline Assay Kit. For quantitative determination of hydroxyproline and collagen in biologic and cosmetic samples.
For quantitative determination of hydroxyproline and collagen in biologic and cosmetic samples. Key Features: Safe. Our hydroxyproline assay uses an improved perchlorate-free chemistry. Sensitive and accurate. Uses 20 µL sample. Linear detection range 0.5 µg/mL to 50 µg/mL hydroxyproline in 96-well plate assay. Convenient. Collagen can be hydrolyzed in Eppendorf tubes, and final incubation can be performed at 37°C. Method: OD560nm. Samples: Hydroxyproline and collagen in biologic and cosmetic samples. Species: All. Procedure: Assay takes Approx. 2 hrs. Kit size: 100 Tests. Detection limit: 0.5 µg/mL.
Eurofins Discoverx cAMP Hunter GPBAR1 Bioassay Kit, 10-Plate. (95-0049Y2-00140)
The cAMP Hunter CHO-K1 GPBAR1 Bioassay Kit provides a robust and highly sensitive functional, cell-based assay to measure drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed to run the assay, including cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure long-term assay reproducibility, and faster implementation from characterization to QC lot release. This bioassay has been optimized for a 96-well format. The protocol can be optimized further for running the assay in a high-throughput 384-well format. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
American Research Products Inc Canine acetylcholine receptor
Canine acetylcholine receptor antibody AChRab ELISA Kit from CUSABIO Detection Range 3 75 ng/mL-240 ng/mL Sensitivity 0 94 ng/mL Sample serum plasma Method Sandwich
Cell Signaling Technology SignalStain Akt Pathway IHC Sampler Kit 1 Kit
SignalStain Akt Pathway IHC Sampler Kit 1 Kit
Cayman Chemical PhagocytosIs asy KItIgG-D 1ea
Cayman’s Phagocytosis Assay Kit (IgG-DyLight™ 633) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the advantage of visualizing perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations. This kit provides enough Latex Beads-Rabbit IgG-DyLight™ 633 Complex for up to 500 samples.
American Research Products Inc Human EXtracellular superoXide
Human EXtracellular superoXide dismutase [Cu-Zn] (SOD3) ELISA kit from CUSABIO Detection Range 7 8 pg/mL-500 pg/mL Sensitivity 1 95 pg/mL Sample serum plasma cell culture supernates tissue homogenates saliva urine Method Sandwich
Eurofins Discoverx HitHunter cAMP Assay for Biologics, 200/800 dp (96-well/384-well). (90-0075LM2)
This detection kit is a robust, sensitive and easy-to-use assay to determine GPCR activity through detection of intracellular cAMP levels. The HitHunter cAMP assay is intended for use with cells expressing Gαs- or Gαi-coupled GPCRs. This product uses EFC technology, and contains ED-tagged cAMP, anti-cAMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cAMP, the anti-cAMP antibody will be sequestered by binding to this untagged cAMP, allowing for EFC between the tagged cAMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit format is intended for Biologic drugs with a protocol optimized for use in a 96-well plate. This kit may be used with Eurofins DiscoverX cAMP cell lines as well as cell lines from other sources. This detection kit contains reagents for assaying 2 plates (96-well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Eurofins Discoverx PathHunter Human Syk Kinase Activity Cell-Based Assay Kit (U2OS)
The PathHunter Human Syk Stable Cell Line Kinase Activity Assay (U2OS) measures SYK CTK activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human TrkC-P75 Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human TrkC-P75 Stable Cell Line Functional Kinase Assay (U2OS) measures TrkC-P75 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.