Cell Growth and Differentiation Reagents and Kits
Biotium Fluo-4, Pentapotassium Salt
Fluo-4, pentapotassium is a membrane-impermeant green-fluorescent calcium indicator that can be loaded into cells via microinjection or scrape loading. It is an improved analog of Fluo-3 with the two chlorine substituents replaced by fluorines, resulting in increased fluorescence excitation at 488 nm and higher fluorescence signal. Fluo-4 is essentially nonfluorescent without Ca2+ but upon Ca2+ binding, its fluorescence increases at least 100 times. Fluo-4 has a higher Kd than fura-2 and indo-1, it is more useful for measuring high transient Ca2+ concentration during Ca2+ spikes. We also offer a membrane-permeant version, Fluo-4 AM Ester.Features: Kd: ~335 nM; Ex/Em nm (low or high [Ca2+]) = 494/516 nm, excitable by the argon-ion laser; Ext. coeff. (494 nm) = 82,000 M-1 cm-1; Orange solid soluble in DMSO and water (pH >6); Store at 4°C. Protect from light, especially when in solution; C36H25F2K5N2O13; MW: 927.09
Biotium Fluo-4, AM ester
Fluo-4 AM Ester is the membrane-permeant form of Fluo-4. Fluo-4 AM ester itself does not bind Ca2+, but is readily hydrolyzed to Fluo-4 by intracellular esterases. Fluo-4, essentially nonfluorescent without Ca2, , shows an increase in fluorescence at least 100 times upon Ca2+ binding. It has a higher Kd than fura-2 and indo-1, making it more useful for measuring high transient intracellular Ca2+ concentrations during Ca2+ spikes. It can be used in high-throughput drug screening assays in live cells, and is detectable using fluorescence microscopy, flow cytometry, or fluorescence microplate reader. We also offer Fluo-4 as a membrane-impermeant pentapotassium salt version, Cat # 50019Features: Orange red solid soluble in DMSO; Store -20°C protected from light, especially in solution; Ex/Em nm (with Ca2+): 494/506 nm (after hydrolysis), excitable by the argon-ion laser; Kd for Ca2+ in buffer: ~335 nM (after hydrolysis); C51H50F2N2O23; MW: 1096.95; [273221-67-3]
Abcam Cell Counting Kit 8 (WST-8 / CCK8)
Cell Counting Kit 8 (WST-8 / CCK8)
The product is subject to the following: Abcam Restricted Use Statement
BioVendor, LLC Purple-Jelley Hyaluronan Assay (Large Economy Kit Size: 400 assays), multispecies colorimetric assay for hyaluronic acid in mammalian tissue, Biocolor Item # H2000
Purple-Jelley Hyaluronan Assay (Large Economy Kit Size: 400 assays). Multispecies colorimetric assay. The Purple-Jelley Hyaluronan Assay is used to measure the quantity of hyaluronan (hyaluronic acid, HA) in mammalian tissue. It is a dye-based method with a detection limit of 0.2 g. The purple dye reagent (3,3-Diethyl-9-methyl-4,5,4,5-dibenzothia carbocyanine bromide) is supplied in 55% solution of dimethyl sulfoxide (DMSO) in a 20 ml vial. Biocolor Item # H2000
BioVendor, LLC Blyscan Sgag 110 Assay Stdsize BioVendor LLC
TypeColorimetric assayApplicationsCell culture and/or animal studies, Urine, Synovial fluid, Tissue extractSample Requirements10 ¿ 100 ¿lShippingAt ambient temperature. Upon receipt, store the product at the temperature recommended below.Storage/ExpirationAll components are stable for one year, (from Invoice Date), when stored at 15¿25¿C. Do not store below +4¿C. Once opened the glass vial containing sGAG standard should however be stored at +4¿C. Calibration Range1 ¿ 5 ¿gLimit of Detection0.25 ¿g
New England Biolabs, Inc. α1-3,6 Galactosidase - 100 units
1-3,6 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of 1-3 and 1-6 linked galactose residues from oligosaccharides.
Abcam MTS Assay Kit (Cell Proliferation) (Colorimetric)
MTS Assay Kit (Cell Proliferation) (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Cell Viability¿Assay Kit (Fluorometric - Blue)
Cell Viability¿Assay Kit (Fluorometric - Blue)
The product is subject to the following: Abcam Restricted Use Statement
Biotium Resazurin Cell Viability Assay Kit (2500 assays)
Resazurin Cell Viability Assay offers a rapid, reliable, sensitive, and cost-effective measurement of cell viability. Its performance is comparable to other commercial resazurin-based kits such as alamarBlue, PrestoBlue, or CellTiter-Blue. Cell growth maintains a reducing cellular environment while growth inhibition makes it oxidizing. Resazurin, a redox indicator, measures cell viability by monitoring conversion of oxidized, non-fluorescent resazurin (purple) to the reduced, highly red-fluorescent (blue) resorufin. The fluorescent (Ex/Em 530-560 nm/590 nm) or colorimetric (Abs. 570-600 nm) signal is proportional to the number of live, metabolically active cells. The ready-to-use, single-step, no-wash, no-lysis, homogenous assay can accurately measure as few as 80 cells. Non-toxic reagents allow long-term kinetic monitoring. The assay is non-radioactive, and non-carcinogenic and compatible with a variety of cell types including bacteria, protozoa, yeast, fungi, or mammalian cells.
Biotium Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit (TO and Live-or-Dye™ 568/583)
The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583. Live-or-Dye™ 568/583 is a fixable red dye, designed for discrimination between live and dead cells during flow cytometry or microscopy. This dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. Thiazole Orange is a green fluorescent dye that localizes to the cytoplasm, with some nuclear accumulation. It is fixable, resulting in diffuse cytoplasmic staining in fixed cells. After staining yeast with this kit, both living and dead cells will be stained with green fluorescence (Ex/Em 512/533 nm). Only dead cells will be stained with red fluorescence (Ex/Em 568/583 nm), and may appear yellow when the two images are overlaid. This kit has sufficient reagent for 1000 assays.
Biotium Calcein AM Cell Viability Assay Kit (1000 assays)
Calcein AM is a widely used membrane-permeant green fluorescent cell marker and thus can be introduced into cells via incubation. Once inside the cells, calcein AM, a nonfluorescent molecule itself, is hydrolyzed by endogenous esterase into the highly negatively charged green fluorescent calcein. Green fluorescent calcein is retained in the cytoplasm in live cells. Calcein AM has served as a useful tool for the studies of cell membrane integrity and for cell tracing. The Calcein AM Cell Viability Assay Kit is designed to quantify live cell numbers based on their endogenous esterase activity and plasma membrane integrity. It is a true end-point assay for cell viability and requires only 30 minutes. The fluorescent signal is monitored using 485 nm excitation wavelength and 530 nm emission wavelength. The fluorescent signal generated from the assay is proportional to the number of living cells in the sample. The kit has reagents sufficient for 1000 assays in a 96-well format.
Bioworld HUMAN PEROXISOME PROLIFERAT
HUMAN PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA (RECOMBINANT): Recombinant Human Peroxisome Proliferator Activated Receptor Gamma (PPAR-g) Source: E.Coli Background : The peroxisome proliferator activated receptors (PPARs) are...
Apexbio Technology LLC ALAMAR BLUE CELL VIA. KIT 5ML
ALAMAR BLUE CELL VIA. KIT 5ML APEXBIO TECHNOLOGIES