Signal Transduction Reagents and Kits
Promega IKK beta Kinase Enzyme System, 1mg
Full length recombinant human IKKβ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega GSH-Glo™ Glutathione Assay
The GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH) in cells or in various biological samples.
Promega MSK1 Kinase Enzyme System, 10microg
Recombinant full-length human MSK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega SIK Kinase Enzyme System, 10microg
Recombinant human SIK (1-303) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. SIK is a protein kinase that is involved in regulating AMPK-related kinases.
Promega ONE-Glo™ Assay
The ONE-Glo™ + Tox Assay combines luciferase assay chemistry with a cell viability marker to better understand reporter gene expression in the context of cell health. The assay uses a two-step, addition-only process to measure in a single well of a plate.
Promega TGF beta R1 Kinase Enzyme System, 1mg
TGF beta R1 is a member of the TGF beta receptor subfamily and is a ser/thr protein kinase that forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF beta signal from the cell surface to the cytoplasm.
Promega ADP-Glo™ Kinase Assay + CDK1/CyclinA2 Kinase Enzyme System
Recombinant full-length human CDK1 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK1 is essential for completion of START, controlling event in the cell cycle required to initiate mitosis.
Promega ADP-Glo™ Kinase Assay + CDK9/CyclinK Kinase Enzyme System
Recombinant full-length human CDK9 and CyclinK proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CDK9/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family.
Promega ADP-Glo™ Kinase Assay + PKCε Kinase Enzyme System
Recombinant full-length human PKC epsilon was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC epsilon is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
Promega ADP-Glo™ Kinase Assay + ALK2 Kinase Enzyme System
ALK2 is a receptor serine/threonine kinase that is member of the ALK family and is upstream of signaling pathway involving the SMAD proteins especially SMAD1/5/8.
Promega ABL1 (E255K) Kinase Enzyme System, 10microg
Recombinant human ABL1 (E255K) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega SRC Kinase Enzyme System, 1mg
Recombinant full-length human SRC was expressed in E. coli cells using an N-terminal GST tag. SRC was originally identified as a transforming protein of the Rous sarcoma virus that had enzymatic ability to phosphorylate tyrosine in protein substrates.
Promega ERK2 Kinase Enzyme System, 1mg
Recombinant full-length human ERK2 was expressed by E. coli cells using an N-terminal GST tag and activated by MEK1 in vitro. ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
Promega Glutamate-Glo Assay, 50ml
The Glutamate-Glo Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamate in biological samples. The assay couples glutamate oxidation and NADH production with a bioluminescent NADH detection system.
Promega ADP-Glo™ Kinase Assay + TGFβR1 Kinase Enzyme System
TGF beta R1 is a member of the TGF beta receptor subfamily and is a ser/thr protein kinase that forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF beta signal from the cell surface to the cytoplasm.