Signal Transduction Reagents and Kits
Promega FLT3 Kinase Enzyme System, 1mg
FLT3 is a receptor tyrosine kinase that has been shown to play a role in proliferation and survival of hematopoietic progenitor cells as well as differentiation of early B lymphoid progenitors.
Promega STK33 Kinase Enzyme System, 1mg
Recombinant full-length human STK33 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. STK33 is a distant member of the CAMK group of serine/threonine kinases.
Promega PRKG1 (PKG) Kinase Enzyme System, 1mg
Recombinant full-length human PRKG1 (PKG) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PRKG1 is a homodimer, with each monomer containing a regulatory cGMP-binding domain and a catalytic domain.
Promega FYN A Kinase Enzyme System, 1mg
Full-length recombinant human FYN A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FYN A (Fyn isoform A) is a member of the SRC tyrosine kinase oncogene family showing high homology to YES1, FGR and SRC.
Promega ADP-Glo™ Kinase Assay + AMPK (A2/B1/G1) Kinase Enzyme System
Recombinant full-length human AMPK (A2/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for diabetes treatment.
Promega ALK2 Kinase Enzyme System, 1mg
ALK2 is a receptor serine/threonine kinase that is member of the ALK family and is upstream of signaling pathway involving the SMAD proteins especially SMAD1/5/8.
Promega CLK3 Kinase Enzyme System, 1mg
Recombinant full-length human CLK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CLK3, also known as CDC-like kinase 3, encodes a serine/threonine-type protein kinase with a non-conserved N-terminal domain.
Promega JNK1 Kinase Enzyme System, 10microg
Recombinant full-length human JNK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JNK1 is a member of the MAP kinase group that is activated by dual phosphorylation at the thr and tyr residues during exposure to stress.
Promega ADP-Glo™ Kinase Assay + NIK Kinase Enzyme System
Recombinant human NIK (325-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NIK is a mitogen-activated protein kinase kinase kinase 14 (MAP3K14), which binds to TRAF2 and stimulates NF-kappaB activity.
Promega ADP-Glo Kinase Assay + ASK1 Kinase Enzyme System, 1 each
Recombinant human ASK1 (649-946) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1) and MKK6. Overexpression of ASK1 induces apoptotic cell death.
Promega PAK1/CDC42 Kinase Enzyme System, 1mg
Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag and combined with CDC42 at a ratio of 1:4 (PAK1:CDC42) in vitro. PAK1 is a member of the p21-activated kinases (PAKs).
Promega MAPKAPK3 Kinase Enzyme System, 1mg
Recombinant full-length human MAPKAPK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK3 is closely related to MAPKAPK2 sharing 72% nucleotide and 75% amino acid identity.
Promega SLK Kinase Enzyme System, 1mg
Recombinant full-length human SLK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SLK or LOSK is a Ste20-like protein kinase that associates with microtubule and centrosome.
Promega PI3K (p110alpha/p85alpha), 20ug, 200microl
The PI3K lipid kinase enzymes are used with ADP-Glo KInase Assay System and Lipid Kinase Substrates in a homogeneous, add-mix-read lipid kinase assay.
Promega ADP-Glo™ Kinase Assay + PKA Kinase Enzyme System
Recombinant bovine cAMP-Dependent Protein Kinase (PKA), Catalytic Subunit, is expressed and purified from E. coli. PKA does not require cAMP for activity, and the gene sequence can be found at accession number NM_174584.