Signal Transduction Reagents and Kits
Promega P450-Glo™ CYP1A2 Induction/Inhibition Assay
The P450-Glo™ CYP1A2 Assay and Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays
Promega GSH-Glo™ Glutathione Assay
The GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH) in cells or in various biological samples.
Promega ADP-Glo™ Kinase Assay + ULK1 Kinase Enzyme System
Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ULK1 is a serine/threonine protein kinase that plays a critical role during initial stages of autophagy, a vital response to nutrient starvation.
Promega p70S6Kb Kinase Enzyme System, 1mg
Recombinant full-length human p70S6Kb was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p70S6Kb is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases and is activated by mitogenic stimuli.
Promega ADP-Glo™ Kinase Assay
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase. The luminescent signal positively correlates with kinase activity.
Promega TBK1 Kinase Enzyme System, 1mg
Recombinant full-length human TBK1 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. TBK1, also known as NAK or NFkB-activating kinase, is an upstream protein kinase that can phosphorylate and activate the IkB kinases.
Promega CSK Kinase Enzyme System, 1mg
Recombinant full-length human CSK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CSK has been shown to downregulate the tyrosine kinase activity of c-src through tyrosine phosphorylation of the c-src carboxy terminus.
Promega ADP-Glo™ Kinase Assay + CAMKK1 Kinase Enzyme System
CAMKK1 or CAMKK alpha is a Ca2+/calmodulin-dependent protein kinase that activates CAM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively.
Promega PI3K (p120gamma), 20ug, 200microl
The PI3K lipid kinase enzymes are used with ADP-Glo KInase Assay System and Lipid Kinase Substrates in a homogeneous, add-mix-read lipid kinase assay.
Promega JAK3 Kinase Enzyme System, 1mg
Recombinant human JAK3 (amino acids 781-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JAK3 is a member of the JAK family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction.
Promega PI3K (p110delta/p85alpha), 20ug, 200microl
The PI3K lipid kinase enzymes are used with ADP-Glo KInase Assay System and Lipid Kinase Substrates in a homogeneous, add-mix-read lipid kinase assay.
Promega Aurora A Kinase Enzyme System, 10microg
Recombinant full-length human Aurora A was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. Aurora A belongs to a multigenic family of mitotic serine/threonine kinases, which are involved in the control of chromosome segregation.
Promega PI:3PS Lipid Kinase Substrate, 0.5mg, 0.5ml
The lipid kinase substrates PIP2:3PS and PI:3PS are standalone substrates that are also part of the PI3K-Glo kinase profiling system.
Promega ERK1 Kinase Enzyme System, 10microg
Recombinant full-length, tag-free human ERK1 was expressed by baculovirus in Sf9 insect cells and activated by active MEK1 in vitro. ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
Promega PIP2:3PS Lipid Kinase Substrate, 0.25mg, 0.25ml
The lipid kinase substrates PIP2:3PS and PI:3PS are standalone substrates that are also part of the PI3K-Glo kinase profiling system.