Signal Transduction Reagents and Kits
Promega Kinase Selectivity Profiling System: AGC-2 + ADP-Glo™ Assay
Easy-to-use kits for performing kinase selectivity profiling that rely on the ADP-Glo™ Kinase Assay technology. Each system includes kinase and substrate pairs in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.
Promega ADP-Glo™ Kinase Assay + NEK2 Kinase Enzyme System
Recombinant full-length human NEK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans.
Promega ADP-Glo™ Kinase Assay + ERK1 Kinase Enzyme System
Recombinant full-length, tag-free human ERK1 was expressed by baculovirus in Sf9 insect cells and activated by active MEK1 in vitro. ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
Promega ADP-Glo™ Kinase Assay + GRK5 Kinase Enzyme System
Recombinant full-length human GRK5 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. GRK5 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family.
Promega ADP-Glo™ Kinase Assay + IRAK4 Kinase Enzyme System
Recombinant full-length human IRAK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Interleukin-1 receptor-associated kinase 4 is an important mediator in the signal transduction of Toll-like receptor and IL1R family members.
Promega ADP-Glo™ Kinase Assay + CDK5/p25 Kinase Enzyme System
Recombinant full-length human CDK5 and p25 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain.
Promega Glutamine/Glutamate-Glo Assay, 5ml
The Glutamine/Glutamate-Glo Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamine and glutamate in biological samples. The assay is based on the conversion of glutamine to glutamate by Glutaminase enzyme.
Promega Luciferin Detection Reagent
A reagent to detect presence of pro-luciferin substrates used to monitor the activity of specific isoforms of cytochrome P450 or NAT2.
Promega ADP-Glo™ Kinase Assay + TGFβR2 Kinase Enzyme System
Recombinant human TGF beta R2 (190-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. TGF beta R2 is a member of the TGF beta receptor subfamily and is a ser/thr protein kinase.
Promega P450-Glo CYP2C19 Screening System, 1,000 assays
The P450-Glo Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays
Promega ADP-Glo Kinase Assay + AKT2 Kinase Enzyme System, 1 each
Recombinant full-length human AKT2 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. AKT2 or Protein Kinase B beta (PKB beta) is a serine/threonine kinase that is a member of the AKT family.
Promega ADP-Glo™ Kinase Assay + CDK2/CyclinA2 Kinase Enzyme System
Recombinant full-length human CDK2 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed.
Promega ADP-Glo™ Kinase Assay + LCK Kinase Enzyme System
Recombinant full-length human LCK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. LCK (p56lck) is a member of the src family of nonreceptor tyrosine kinases.
Promega PIP2:3PS Lipid Kinase Substrate, 0.25mg, 0.25ml
The lipid kinase substrates PIP2:3PS and PI:3PS are standalone substrates that are also part of the PI3K-Glo kinase profiling system.
Promega PI3K (p110beta/p85alpha), 20ug, 200microl
The PI3K lipid kinase enzymes are used with ADP-Glo KInase Assay System and Lipid Kinase Substrates in a homogeneous, add-mix-read lipid kinase assay.