Signal Transduction Reagents and Kits
Promega Luciferin Detection Reagent with Esterase
A reagent to detect presence of pro-luciferin substrates used to monitor the activity of specific isoforms of cytochrome P450 or NAT2.
Promega ALK4 Kinase Enzyme System, 10microg
Recombinant human ALK4 (150-end) was expressed in Sf9 cells using an N-terminal GST tag. ALK4 is a member of the subfamily of receptor ser/thr kinases that mediates signaling by the Activins.
Promega ADP-Glo™ Kinase Assay + DNA-PK Kinase Enzyme System
Native human DNA-Dependent Protein Kinase purified from HeLa cells. Consists of a ∼460kDa catalytic subunit and a heterodimeric DNA-binding subunit (Ku) containing an 85kDa and a 70kDa peptide (1). Gene sequence can be found at accession number NM_006904.
Promega ADP-Glo™ Kinase Assay + c-KIT Kinase Enzyme System
Recombinant human c-KIT (544-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. c-KIT is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor).
Promega LRRK2 Kinase Enzyme System, 10microg
LRRK2 or leucine-rich repeat kinase is a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain and a WD40 domain.
Promega CAMK1 gamma Kinase Enzyme System, 1mg
Full-length recombinant human CAMK1 gamma was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CAMK1 gamma (CLICK-III), a member of the CAMK family, is a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase.
Promega RET Kinase Enzyme System, 1mg
Recombinant human RET (amino acids 658-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RET gene codes for a transmembrane tyrosine kinase.
Promega PKC beta I Kinase Enzyme System, 10microg
Recombinant full-length human PKCbeta I was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKCβI is a member of the PKC family (phospholipid-dependent serine/threonine kinase) and is highly related to PKCβ II.
Promega ADP-Glo™ Kinase Assay + PAK3 Kinase Enzyme System
Recombinant full-length mouse PAK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PAK3 is a member of the family of ser/thr p21-activating kinases that serve as targets for the small GTP-binding proteins Cdc42 and RAC.
Promega ADP-Glo™ Kinase Assay + CDK3/CyclinE1 Kinase Enzyme System
Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK3/CyclinE1 is a member of the cyclin-dependent protein kinase family.
Promega ADP-Glo™ Kinase Assay + PDGFRα (D842V) Kinase Enzyme System
Recombinant human PDGFR alpha (D842V) (550-end) point mutant was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PDGFR alpha is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity.
Promega PDGFR alpha (T674I) Kinase Enzyme System, 10microg
Recombinant human PDGFRα (Y674I) (550-end) point mutant was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + LRRK2 Kinase Enzyme System
LRRK2 or leucine-rich repeat kinase is a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a mLK-like domain and a WD40 domain.
Promega PIM2 Kinase Enzyme System, 10microg
Recombinant full-length human PIM2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PIM2 is a serine threonine kinase that is present in all tissues.
Promega TRKB Kinase Enzyme System, 10microg
Recombinant human TRKB (455-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TRKB is a member of the neurotrophic tyrosine receptor kinase (NTRK) family.