Signal Transduction Reagents and Kits
Promega ADP-Glo™ Kinase Assay + TGFβR2 Kinase Enzyme System
Recombinant human TGF beta R2 (190-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. TGF beta R2 is a member of the TGF beta receptor subfamily and is a ser/thr protein kinase.
Promega ADP-Glo™ Kinase Assay + ERK2 Kinase Enzyme System
Recombinant full-length human ERK2 was expressed by E. coli cells using an N-terminal GST tag and activated by MEK1 in vitro. ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
Promega ADP-Glo™ Kinase Assay + CDK6/CyclinD3 Kinase Enzyme System
Recombinant full-length human CDK6 and CyclinD3 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Promega ADP-Glo™ Kinase Assay + ALK6 Kinase Enzyme System
ALK6 (also known as BMPR1B) is a member of the transmembrane serine/threonine kinase that is the member of the bone morphogenetic protein (BMP) receptor, which is closely related to the activin receptors, ACVR1 and ACVR2.
Promega SIK Kinase Enzyme System, 1mg
Recombinant human SIK (1-303) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. SIK is a protein kinase that is involved in regulating AMPK-related kinases.
Promega CDC7/DBF4 Kinase Enzyme System, 1mg
Recombinant full-length human CDC7 and DBF4 proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + CDK9/CyclinK Kinase Enzyme System
Recombinant full-length human CDK9 and CyclinK proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CDK9/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family.
Promega ADP-Glo™ Kinase Assay + TRKB Kinase Enzyme System
Recombinant human TRKB (455-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TRKB is a member of the neurotrophic tyrosine receptor kinase (NTRK) family.
Promega ADP-Glo™ Kinase Assay + MSK1 Kinase Enzyme System
Recombinant full-length human MSK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega AMPK (A2/B1/G1) Kinase Enzyme System, 10microg
Recombinant full-length human AMPK (A2/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for diabetes treatment.
Promega Caspase-Glo 1 Inflammasome Assay, 5 x 10ml
The Caspase-Glo 1 Inflammasome Assay is a homogeneous, bioluminescent method to selectively measure the activity of caspase-1, a member of the cysteine aspartic acid-specific protease (caspase) family and an essential component of the inflammasome.
Enzo Life Sciences SAG, (1mg), CAS Number: 364590-63-6
Smoothened ligand. A potent cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (KD = 59 nM). Shown to induce Hedgehog pathway activation (EC50= ~3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine (BML-GR334) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations. Formula: C28H28ClN3OS. MW: 490.1. CAS Number: 364590-63-6. Purity: ≥95% (HR-MS, 1H-NMR, 13C-NMR). Appearance: White to off-white solid. Solubility: Soluble in DMSO (10mg/ml), sparingly soluble in water (acidification with HCl increases water solubility). Use/Stability: Stable for at least 6 months when stored at -20°C. Long Term Storage: -20°C
Aat Bioquest Calbryte™ 520 AM | 1mg
Calbryte™ 520, AM is a novel fluorescent and cell-permeable indicator for the measurement of intracellular calcium. Like other dye AM esters, Calbryte™ 520 AM is non-fluorescent and non-activatable. Once Calbryte™ 520 AM enters the cell, it is readily hydrolyzed by intracellular esterase where it becomes activated and responsive to calcium. The activated indicator is now a polar molecule that is incapable of freely diffusing through the cell membrane, essentially trapping it inside the cell. Upon binding calcium ions, Calbryte™ 520 produces a bright fluorescence signal with extremely high signal/background ratio. It has the identical excitation and emission wavelength as Fluo-4, thus the same Fluo-4 assay settings can be readily applied to Calbryte™ 520-based calcium assays.