Enzymes and Inhibitors
New England Biolabs, Inc. Nb.BsmI – 1000 units
A restriction endonuclease that recognizes the sequence GAATG_CN.
Rockland Immunochemicals Lysozyme (Hen Egg White) - MB-109-1000
Lysozyme is a from a family of enzymes that act upon glycoproteins in the cell walls of bacteria. Lysozyme is a highly abundant protein in a variety of secretions: tears, saliva, milk, and mucus (in mammals). The whites of avian eggs are also an abundant source of lysozyme.
New England Biolabs, Inc. Salt-T4 DNA Ligase – 100000 units
A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase. This enzyme will join cohesive end termini at salt concentrations as high as 300 mM without any loss in activity. This enzyme is insensitive to salt carried over from other reaction components (vector or insert DNA) and allows ligation reactions to proceed in alternative reaction buffers with higher levels of salt (e.g., NEBuffer r3.1).
Discovery Life Sciences MAO B SUPERSOME
Human Monoamine Oxidase B (MAO-B) 0.5mL This activity is catalyzed by MAO-B which is expressed from human MAO-B cDNA using a baculovirus expression system
Genscript Corporation GenCrispr T7 Endonuclease I
T7 Endonuclease I (T7E1) recognizes and cleaves non-perfectly matched DNA cruciform DNA structures Holliday structures or junctions hetero duplex DNA and more slowly nicked double-stranded DNA The cleavage site is at the first second or third phosphodiester bond that is 5 to the mismatch The protein is the product of T7 gene 3 GenCrispr T7 Endonuclease I is a fusion protein produced from i E coli /i
Genscript Corporation GenCrispr T7 Endonuclease I
T7 Endonuclease I (T7E1) recognizes and cleaves non-perfectly matched DNA cruciform DNA structures Holliday structures or junctions hetero duplex DNA and more slowly nicked double-stranded DNA The cleavage site is at the first second or third phosphodiester bond that is 5 to the mismatch The protein is the product of T7 gene 3 GenCrispr T7 Endonuclease I is a fusion protein produced from i E coli /i
New England Biolabs, Inc. Hi-T4™ DNA Ligase – 20000 units
A thermotolerant variant of T4 DNA Ligase, Hi-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher temperatures than wild type T4 DNA Ligase. Hi-T4 DNA Ligase will join blunt end and cohesive end termini as well as repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids at temperatures as high as 50C.
New England Biolabs, Inc. Salt-T4 DNA Ligase – 20000 units
A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase. This enzyme will join cohesive end termini at salt concentrations as high as 300 mM without any loss in activity. This enzyme is insensitive to salt carried over from other reaction components (vector or insert DNA) and allows ligation reactions to proceed in alternative reaction buffers with higher levels of salt (e.g., NEBuffer r3.1).
Apexbio Technology LLC Dpn I(Synonyms: DpnI, DpnI Restriction Enzyme, Dam methylation-sensitive restriction enzyme DpnI), 5000U.
Dpn I is a restriction endonuclease sensitive to Dam methylation specifically recognizing and cleaving the adenine N6-methylated sequence GA/TC within DNA Cleavage is dependent on adenine methylation status mammalian CpG methylation overlapping Dpn I sites blocks cleavage and methylation by Dcm is not recognized by this enzyme It is routinely used for the rapid digestion of plasmid DNA PCR fragments and genomic DNA and finds broad utility in genetic typing molecular cloning Southern blotting SNP genotyping and restriction fragment length polymorphism (RFLP) analyses
Takara Bio ADVANTAGE HD POLYMERASE MIX
Advantage HD DNA Polymerase Mix: this High fidelity PCR enzyme provides superb accuracy and efficiency.