Enzymes and Inhibitors
New England Biolabs, Inc. Nb.BsmI – 1000 units
A restriction endonuclease that recognizes the sequence GAATG_CN.
New England Biolabs, Inc. Nb.BtsI – 1000 units
A restriction endonuclease that recognizes the sequence GCAGTG_NN.
New England Biolabs, Inc. SphI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence GCATG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
Aniara Diagnostica LLC BIOPHEN CS-01(38) THROMB 12 EA
This item MFG by HYPHEN BioMed and Dis. by Aniara Diagnostics. Chromogenic substrate for thrombin, lyophilized. Peptide sequence H-D-Phe-Pip-Arg-pNa, 2HCl. 12 vials x 25 mg.
Supply Solutions Roche - RNase A, 100 mg; from bovine pancreas; pkg of 100 mg (10109169001) pkg of 25 mg (10109142001)
Roche - RNase A, 100 mg; from bovine pancreas; pkg of 100 mg (10109169001) pkg of 25 mg (10109142001)
Supply Solutions Roche - Rapid DNA Dephos & Ligation Kit 40 Rxns
Roche - Rapid DNA Dephos & Ligation Kit 40 Rxns
New England Biolabs, Inc. SalI-HF – 2000 units
A high fidelity restriction endonuclease that recognizes the sequence G^TCGAC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Supply Solutions Roche - Expand HiFi PCR Sys., 500 u; pkg of 2,500 U (11759078001 [10x250 U]) pkg of 100 U (11732641001) pkg of 500 U (11732650001 [2x250 U])
Roche - Expand HiFi PCR Sys., 500 u; pkg of 2,500 U (11759078001 [10x250 U]) pkg of 100 U (11732641001) pkg of 500 U (11732650001 [2x250 U])
Sigma Aldrich Fine Chemicals Biosciences Endoglycosidase F2 from Elizabethkingia miricola recombinant, expressed in E. coli, 20 U/mg | 37278-88-9 | MFCD00151069 | 0.2UN
Endoglycosidase F2 from Elizabethkingia miricola recombinant, expressed in E. coli, 20 U/mg | 37278-88-9 | MFCD00151069 | 0.2UN
New England Biolabs, Inc. Endoproteinase AspN – 50 µg
Endoproteinase AspN (flavastacin) is a zinc metalloendopeptidase which selectively cleaves protein and peptide bonds N-terminal to aspartic acid residues.
New England Biolabs, Inc. BspQI – 2500 units
A restriction endonuclease that recognizes the sequence GCTCTTCN^NNN_.
New England Biolabs, Inc. ScaI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence AGT^ACT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
New England Biolabs, Inc. Micrococcal Nuclease – 320000 gel units
Micrococcal nuclease is derived from Staphylococcus aureus and is a relatively non-specific endo-exonuclease. It is purified from a recombinant E. coli strain that digests double-stranded, single-stranded, circular and linear nucleic acids. The enzyme is active in the pH range of 7.0 - 10.0, with optimal activity at pH 9.2 for both RNA and DNA substrates. Cleavage preferences have been observed at sites rich in adenylate, deoxyadenylate or thymidylate. Both DNA and RNA are degraded to 3 phosphomononucleotides and dinucleotides. Micrococcal Nuclease works well with the SimpleChIP Enzymatic Chromatin IP Kit (Agarose Beads) from Cell Signaling Technology.