Enzymes and Inhibitors
Biotium Coelenterazine HCP
Coelenterazine hcp is a synthetic derivative of coelenterazine. Its aequorin complex generates luminescence intensity 190 times higher and has a faster response time to calcium compared to native coelenterazine.Coelenterazines are luminescent enzyme substrates for apoaequorin and Renilla luciferase. Apoaequorin is used as a calcium indicator. It binds coelenterazine to form an aequorin complex that emits light upon calcium binding and measures concentrations ~0.1 uM to >100 uM. Renilla luciferase is widely used as a reporter of transcription regulation and as a donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Coelenterazines are also used for chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues. Coelentrazine analogs and water-soluble Aquaphile™ Coelenterazines are also available.Properties : Yellow solid soluble in MeOH or EtOH; Store at -20°C, protect from light; C25H25N3O2; MW: 399.49.
Sigma Aldrich Fine Chemicals Biosciences Protease from Streptomyces griseus, 9036-06-0, MFCD00132092, 1 g
Type XIV, ≥3.5 units/mg solid, powder, Synonym: Actinase E, Pronase E. Highly stable in pH range of 5.0 to 9.0, with peak activity at pH 8.8. Compatible with many DNA and RNA isolation buffers. Broad substrate specificity.
Carnabio Usa Inc MST2(STK3)/5UG/FULL-LENGTH
MST2(STK3), Full-length, Wild type, Amino Acid 1-491, Q13188, Expressed in Insect (sf21); 5microg
New England Biolabs, Inc. RNase HII – 250 units
Ribonuclease HII (RNase HII) is an endoribonuclease that preferentially nicks 5' to a ribonucleotide within the context of a DNA duplex. The enzyme leaves 5' phosphate and 3' hydroxyl ends. RNase HII will also nick at multiple sites along the RNA portion of an Okazaki fragment.
New England Biolabs, Inc. NEBExpress T4 Lysozyme
NEBExpress T4 Lysozyme is a recombinant murein hydrolase that breaks down the bacterial cell wall by hydrolyzing the beta 1-4 linkage between N-acetylmuramic acid and N-acetylglucosamine in the peptidoglycan of prokaryotic cells (gram-negative and some gram-positive bacteria). It can be used to extract soluble proteins, membrane proteins, DNA, RNA, or metabolites.
New England Biolabs, Inc. BspHI – 500 units
A restriction endonuclease that recognizes the sequence T^CATG_A.
New England Biolabs, Inc. Vent DNA Polymerase – 200 units
Vent DNA Polymerase is a high-fidelity thermophilic DNA polymerase. The fidelity of Vent DNA Polymerase is 5-15-fold higher than that observed for Taq DNA Polymerase. This high fidelity derives in part from an integral 3' to 5' proofreading exonuclease activity in Vent DNA Polymerase. Greater than 90% of the polymerase activity remains following a 1 hour incubation at 95C.