Enzymes and Inhibitors
Bioss KD-Validated Topoisomerase II
DNA Topoisomerase II Alpha Primary Antibody Unconjugated Public Immunogen Range 362-400/400 Host Species Rabbit Target Species Human Mouse Rat Clonality Monoclonal Applications WB FCM IC Concentration Lot dependent Size 50 ul Storage Buffer Supplied in PBS (pH 7 4) containing 50% glycerol and 0 02% sodium azide
New England Biolabs, Inc. Bst DNA Polymerase, Large Fragment – 8000 units
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5' to 3' polymerase activity, but lacks 5' to 3' exonuclease activity.
New England Biolabs, Inc. α-N-Acetylgalactosaminidase - 3000 units
a-N-Acetylgalactosaminidase is an exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing a-N-Acetylgalactosamine residues from oligosaccharides and N-glycans attached to proteins.
Alkali Scientific ASI™ Proteinase K Powder, UltraPure Grade, 1000mg, Store at -20°C
Proteinase K Powder, UltraPure Grade, store at -20°C, 1000mg
Amsbio LLC Heparinase III (EC 4.2.2.8) from Flavobacterium heparinum/ 1 IU
Heparinase III (EC 4.2.2.8) from Flavobacterium heparinum 1 IU
New England Biolabs, Inc. USER® Enzyme – 50 units
USER (Uracil-Specific Excision Reagent) Enzyme generates a single nucleotide gap at the location of a uracil. USER Enzyme is a mixture of Uracil DNA glycosylase (UDG) and the DNA glycosylase-lyase Endonuclease VIII. UDG catalyses the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease VIII breaks the phosphodiester backbone at the 3 and 5 sides of the abasic site so that base-free deoxyribose is released.Applications of USER include:
- Directional RNA Seq
- NEBNext adaptor cleavage
- USER cloning
New England Biolabs, Inc. Lambda Protein Phosphatase – 100000 units
Lambda Protein Phosphatase is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues.
New England Biolabs, Inc. Bst DNA Polymerase, Large Fragment – 8000 units
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5' to 3' polymerase activity, but lacks 5' to 3' exonuclease activity.
New England Biolabs, Inc. ShortCut RNase III – 1000 units
ShortCut RNase III, used with its manganese-containing reaction buffer, converts long double-stranded RNA into a heterogeneous mix of short (18-25 bp) interfering RNAs (siRNA) suitable for RNA interference in mammalian cells. 1.5 units (1 µl) of ShortCut RNase III is sufficient to convert 1µ g of dsRNA into siRNA suitable for RNA interference in mammalian cells.
New England Biolabs, Inc. Exonuclease T – 250 units
Exonuclease T (Exo T), also known as RNase T, is a single-stranded RNA or DNA specific nuclease that requires a free 3' terminus and removes nucleotides in the 3' to 5' direction. Exonuclease T can be used to generate blunt ends from RNA or DNA molecules that have 3 extensions.
New England Biolabs, Inc. Topoisomerase I (E. coli) – 500 units
Topoisomerase I (E. coli) catalyzes the relaxation of negatively supercoiled DNA. Topoisomerase I has also been implicated in knotting and unknotting DNA and in linking complementary rings of single-stranded DNA into double-stranded rings. The intact holoenzyme is a 97 kDa protein.
Worthington Biochemical Corporation PDS KIT 5/PK
Papain Dissociation System, Papain Vial, Code: PAP2, Vial reconstituted with 5mL of EBSS or equivalent yields a solution at 20U of papain permL in 1mm L-cysteine with 0.5mm EDTA, Store at 2 deg. to 8 deg.C, 5Vi
Sigma Aldrich Fine Chemicals Biosciences Catalase from bovine liver aqueous solution, >=30,000 units/mg protein | 9001-05-2 | MFCD00081483 | 50MG
Catalase from bovine liver aqueous solution, >=30,000 units/mg protein | 9001-05-2 | MFCD00081483 | 50MG
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, >=2,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 1KU
Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, >=2,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 1KU
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, >=2,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 2KU
Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, >=2,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 2KU