Enzymes and Inhibitors
Apexbio Technology LLC T7 Endonuclease I(Synonyms: T7EI, T7 Endo I, T7 Endonuclease 1, T7 Endo 1, T7 mismatch-specific endonuclease), 250U.
T7 Endonuclease I (T7EI) is a recombinant DNA endonuclease produced through expression in E coli T7EI specifically targets and cleaves diverse forms of DNA substrates containing structural anomalies including mismatches of at least two base pairs heteroduplex DNA cruciform structures Holliday junctions and branched DNA intermediates as well as nicked duplexes at reduced cleavage efficiency It hydrolyzes phosphodiester bonds located near mismatched nucleotides 5 ends This enzyme is widely employed in detecting genetic mutations induced by gene-editing systems (e g CRISPR/Cas9 TALENs) identifying single nucleotide polymorphisms (SNPs) and generating randomized DNA fragments for shotgun cloning techniques
Bulldog Bio Inc PNGase F PRIME-LY, Glycosidase, 2 x 100 μg vials, lyophilized, total activity 100,000 units, purity ≥95%
PNGase F PRIME-LY, Glycosidase, 2 x 100 μg vials, lyophilized, total activity 100,000 units, purity ≥95%
Haematologic Technologies Inc HUMAN PLASMIN 500UG
Coagulation protein, Human Plasmin, 500 micrograms, Native enzyme, Mode of action: Enzyme involved in fibrinolysis, Research use only, 50% (v/v) glycerol/water, store at -20 Celsius.
MOLECULAR DEPOT LLC Xanthine Oxidase - 100 U
Xanthine Oxidase Grade II (Lyophilized Powder) consists of a highly purified native Xanthine oxidase supplied as a lyophilized form Xanthine oxidase uses molybdenum as cofactor and plays a central role in the metabolism of nucleotides It is extensively used in enzymatic assays for the determination of inorganic phosphorus 5-nucleotidase (5-NT) and adenosine deaminase (ADA) For in vitro research use only
Bioss KD-Validated DNA ligase 4 Rabb
DNA Ligase 4 Primary Antibody Unconjugated Public Immunogen Range 504-685/911 Host Species Rabbit Target Species Human Clonality Monoclonal Applications WB FCM IC Concentration Lot dependent Size 50 ul Storage Buffer Supplied in PBS (pH 7 4) containing 50% glycerol and 0 02% sodium azide
Sigma Aldrich Fine Chemicals Biosciences Adenosine 5'-phosphosulfate sodium salt >=85% | 102029-95-8 | MFCD00057011 | 5MG
Adenosine 5'-phosphosulfate sodium salt >=85% | Purity: >=85% | Mol Wt: 427.28 | 102029-95-8 | MFCD00057011 | 5MG
Medchemexpress LLC ADAMTS-5 Inhibitor 5mg | 929634-33-3 | 5 MG
ADAMTS-5 inhibitor is a small-molecule research compound that selectively inhibits ADAMTS-5 (aggrecanase-2) with an IC50 of 1.1 μM. It is supplied as a high-purity solid for biochemical and cell-based studies.
- Potent ADAMTS-5 inhibition (IC50 1.1 μM).
- Greater than 40-fold functional selectivity over ADAMTS-4.
- High reported purity (99.59%).
- Molecular weight 449.92 g/mol; formula C16H11ClF3N3OS3.
- Available in mg-scale quantities for research use.
Abcam Magenta-Gal, Chromogenic beta-galactosidase substrate, 500MG
MW 408.63 g/mol. Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product. Used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.
The product is subject to the following: Abcam Restricted Use Statement
New England Biolabs, Inc. Human Alkyladenine Glycosylase (hAAG) – 500 units
hAAG excises alkylated and oxidative DNA damaged sites and catalyzes the hydrolysis of the N-glycosidic bond to release the damaged base.
New England Biolabs, Inc. T4 Phage beta-glucosyltransferase – 2500 units
T4 Phage -glucosyltransferase specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA, making beta-glucosyl-5-hydroxymethylcytosine.
New England Biolabs, Inc. RNA 5’ Pyrophosphohydrolase (RppH) – 200 units
The bacterial RNA 5' Pyrophosphohydrolase (RppH) removes pyrophosphate from the 5' end of triphosphorylated RNA to leave a 5' monophosphate RNA. The RppH protein was also known as NudH/YgdP which can split Ap5A to ADP and ATP.
New England Biolabs, Inc. Pyrophosphatase, Inorganic (E. coli) – 50 units
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate:
Takara Bio T4 DNA LIGASE 125000U (1000 W
Enzyme; Takara; T4 DNA Ligase; 5 x 25,000U; 350U/uL; Source: Recombinant E. coli strain
Takara Bio MICROCOCCAL NUCLEASE 15000U
Enzyme; Micrococcal nuclease; 15,000U; Digests single-stranded DNA; Will also digest double-stranded DNA or RNA; Yield 3'-phosphate mononucleotides and oglionucleotides; Requires calcium; Completely inactivated by EDTA and EGTA