Enzymes and Inhibitors
MilliporeSigma™ Calbiochem™ Y-27632
Y-27632A, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.
Nuclease P1, MP Biomedicals™
Exhibits both phosphodiesterase and monoesterase activities. Nuclease P1, MP Biomedicals is a single strand-specific nuclease that exhibits high selectivity for single-stranded nucleic acids and single-stranded regions in double-stranded nucleic acids. De
MP Biomedicals™ Collagenase Type II from Clostridium histolyticum
Collagenase used for collagen structural and biosynthetic studies
Invitrogen™ Anza™ DNA End Repair Kit
Used to convert DNA with cohesive ends to blunt-ended 5'-phosphorylated DNA for use in blunt-end ligation reactions
Invitrogen™ EnzChek™ Cellulase Substrate, blue fluorescent, 339/452
Mixed with enzyme to produce fluorescent reaction
R&D Systems™ Mca-SEVNLDAEFRK(Dnp)RR-NH2 Fluorogenic Peptide Substrate
Applications: Bioactivity
R&D Systems™ Boc-QAR-AMC Fluorogenic Peptide Substrate
Substrate for matriptase, spinesin, and prostasin
MilliporeSigma™ Calbiochem™ 5-Bromo-4-chloro-3-indolyl-α-D-galactopyranoside
Chromogenic substrate for α-galactosidase
MP Biomedicals™ L-Glutamic Dehydrogenase
Derived from Bovine liver
Thermo Scientific™ RsaI (10 U/μL)
The RsaI restriction enzyme recognizes GT^AC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AfaI, CviQI, RsaNI).
Thermo Scientific™ HhaI (10 U/μL)
Cut at GCG^C sites with HhaI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AspLEI, BstHHI, CfoI, HinP1I, HspAI).
Thermo Scientific™ AlfI
Cut at ^(10/12)GCA(N)6TGC(12/10)^ sites with AlfI restriction enzyme, which performs best at 37°C in R (+SAM) buffer.
Thermo Scientific™ TaaI (HpyCH4III) (10 U/μL)
The TaaI (HpyCH4III) restriction enzyme recognizes ACN^GT sites and cuts best at 65°C in Tango buffer (Isoschizomers: Bst4CI, HpyCH4III).
Thermo Scientific™ PsuI (BstYI) (10 U/μL)
Cut at R^GATCY sites with PsuI (BstYI) restriction enzyme, which functions best at 37°C in B buffer (Isoschizomers: BstX2I, BstYI, MflI, XhoII).