Enzymes and Inhibitors
Sigma Aldrich Fine Chemicals Biosciences Pyruvate Kinase from rabbi
Pyruvate Kinase (PK) is a glycolysis enzyme and has four isozymes L R M1 and M2. The L isozyme is localized in gluconeogenic tissues particularly in the liver. Whereas the R and M1 are localized in the adult skeletal muscles heart brain and erythrocytes respectively. M2 is localized in the nucleus of the cells.
Apexbio Technology LLC Phosphatase Inhibitor Cocktail 1 (100X in DMSO) 100x1ml
Many cellular processes are regulated by reversible phosphorylation of proteins and approximately 30% of proteins may become phosphorylated during their lifetime Cellular crude extracts contain numerous endogenous enzymes such as phosphatases and proteases which can degrade and modify proteins present in the extract Addition of inhibitors for these enzymes is the optimal approach for maximizing intact protein yield Phosphatase Inhibitor Cocktail 1 inhibits alkaline phosphatase L-isoenzymes and serine/threonine phosphatases such as protein phosphatases 1 and 2A (PP1 and PP2A) It has been optimized and tested in cell extracts from various animal tissues This Phosphatase Inhibitor Cocktail 1 contains Cantharidin Bromotetramisole and Microcystin LR It is supplied as a ready-to-use solution in DMSO
Boston Bioproducts Inc qPCR Proteinase K Buffer (10X) - 100ml
qPCR Proteinase K Buffer (10X) - 100ml (For Research Use and Further Manufacturing Only)
AdipoGen VX-745
Chemical. CAS 209410-46-8. Formula C19H9Cl2F2N3OS. MW 436.3. VX-745 is a potent inhibitor of p38α MAPK IC50=10nM with 20-fold selectivity over p38β and 1000-fold selectivity over closely related kinases, ERK1, MK2 and JNK1-3. p38α is a member of the mitogen-activated protein kinase MAPK family of serine/threonine protein kinases and is widely expressed in endothelial, immune or inflammatory cells and plays a central role in the regulation of proinflammatory cytokine production including TNF-α, IL-1β and IL-6. VX-745 is an anti-inflammatory agent that blocks IL-1β and TNF-α release in whole blood in vitro with IC50 values of 150 and 180nM, respectively. VX-745 showed anti-inflammatory efficacy in an animal model of rheumatoid arthritis and recently anti-neuroinflammatory potential in prevention of early-stage Alzheimers diseases AD.
Medchemexpress LLC Casein kinase inhibitor A86 | 2079069-01-3 | 99.3% | 344.43 g/mol | C18H25FN6 | 10MM 1ML
Casein kinase inhibitor A86 is a potent small-molecule inhibitor of casein kinase 1α (CK1α) with reported activity against CDK7 and CDK9. Supplied as a 10 mM solution in DMSO, it is intended for biochemical and cell-based research applications, including studies of apoptosis and leukemia-related signaling pathways.
- Potent CK1α inhibitor with activity against CDK7 and CDK9.
- Supplied as a 10 mM solution in DMSO for ready use in assays.
- High purity suitable for research: 99.3%.
- Molecular formula C18H25FN6; molecular weight 344.43 g/mol.
- Volume 1 mL, convenient for small-scale experiments.
- For research use only; not for human therapeutic applications.
Gold Biotechnology Inc Proteinase K RNase DNase free
Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins It cleaves at the carboxylic acid side of aliphatic aromatic or hydrophobic amino acids Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids Proteinase K is also useful for helping to characterize enzymes due to its cleavage specificity This enzyme was designated proteinase K because of its ability to hydrolyze keratin Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH The isoelectric point of proteinase K is 8 9
New England Biolabs, Inc. StickTogether™ DNA Ligase Buffer – 2 ml
New England Biolabs supplies a reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of T3, T7, Hi-T4 and Salt-T4 DNA Ligases .
Apexbio Technology LLC Phosphatase Inhibitor Cocktail 2 (100X in ddH2O) 100x1ml
Many cellular processes are regulated by reversible phosphorylation of proteins and approximately 30% of proteins may become phosphorylated during their lifetime Cellular crude extracts contain numerous endogenous enzymes such as phosphatases and proteases which can degrade and modify proteins present in the extract Addition of inhibitors for these enzymes is the optimal approach for maximizing intact protein yield Phosphatase Inhibitor Cocktail 2 inhibits tyrosine protein phosphatases acid phosphatases and alkaline phosphatases Phosphatase Inhibitor Cocktail 2 has been optimized and tested in cell extracts from multiple animal tissues This Phosphatase Inhibitor Cocktail 2 contains Sodium orthovanadate Sodium molybdate Sodium tartrate Imidazole and Sodium Fluoride It is supplied as a ready-to-use solution in ddH2O
New England Biolabs, Inc. PaqCI - 1000 units
PaqCI is a Type IIS restriction enzyme with a 7 basepair recognition sequence: CACCTGC(4/8). It is sourced from an E. coli strain that carries the modified cloned PaqCI gene from Paucibacter aquatile. PaqCI requires multiple sites for efficient cleavage -- PaqCI Activator is included for optimal cleavage. This enzyme is supplied with NEB's rCutSmart Buffer and is heat inactivated.
New England Biolabs, Inc. EnGen Spy dCas9 (SNAP-tag) – 400 pmol
EnGen Spy dCas9 is an inactive mutant of Cas9 nuclease that retains programmable DNA binding activity. EnGen Spy dCas9 contains two point mutations, D10A and H840A, which inactivate its RuvC and HNH nuclease domains, respectively. The N-terminal SNAP-tag allows for covalent attachment of fluorophores, biotin, and a number of other conjugates useful for visualization and target enrichment. Like with Cas9 nuclease, DNA binding by EnGen Spy dCas9 requires a guide RNA complementary to a target sequence with a protospacer adjacent motif (PAM), NGG, downstream of the target sequence. EnGen Spy dCas9 contains a SV40 T antigen nuclear localization sequence (NLS) at the C- terminus of the protein.
Worthington Biochemical Corporation DEOXYRIBONUCLEASE I FLTRD-25MG
Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for pyrogenicity.
New England Biolabs, Inc. TaqI-v2 – 20000 units
A restriction endonuclease that recognizes the sequence T^CG_A.