Enzymes and Inhibitors
New England Biolabs, Inc. BssHII (25,000 units/ml) – 2500 units
A restriction endonuclease that recognizes the sequence G^CGCG_C.
New England Biolabs, Inc. PleI – 1000 units
A restriction endonuclease that recognizes the sequence GAGTCNNNN^N_.
New England Biolabs, Inc. Bsu36I – 5000 units
A restriction endonuclease that recognizes the sequence CC^TNA_GG.
New England Biolabs, Inc. MscI – 1250 units
A restriction endonuclease that recognizes the sequence TGG_^CCA.
New England Biolabs, Inc. Nt.BstNBI – 1000 units
A restriction endonuclease that recognizes the sequence GAGTCNNNN^N.
New England Biolabs, Inc. BseYI – 500 units
A restriction endonuclease that recognizes the sequence C^CCAG_C.
New England Biolabs, Inc. ApeKI – 1250 units
A restriction endonuclease that recognizes the sequence G^CWG_C.
New England Biolabs, Inc. XbaI – 15000 units
A restriction endonuclease that recognizes the sequence T^CTAG_A.
New England Biolabs, Inc. NotI – 2500 units
A restriction endonuclease that recognizes the sequence GC^GGCC_GC.
New England Biolabs, Inc. DpnII – 5000 units
A restriction endonuclease that recognizes the sequence ^GATC_.
New England Biolabs, Inc. MboI – 500 units
A restriction endonuclease that recognizes the sequence ^GATC_.
New England Biolabs, Inc. ApaLI – 12500 units
A restriction endonuclease that recognizes the sequence G^TGCA_C.
Zymo Research Corporation DNA Degradase™ (500 U)
DNA Degradase™ is ideal for whole-genome DNA methylation analysis by a number of downstream applications (i.e., HPLC, TLC, etc.). Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.DNA Degradase™degrades DNA to individual nucleotides.Quantitation of nucleotides to detect DNA methylation levels can be assessed by various applications (e.g.- TLC, HPLC, etc.). For detection of DNA methylation via LC/MS, DNA needs to be degraded down to individual nucleosides.
New England Biolabs, Inc. SplintR Ligase – 1250 units
SplintR Ligase efficiently catalyzes the ligation of adjacent, single-stranded DNA splinted by a complementary RNA strand. This activity may enable novel approaches for characterization of miRNAs and mRNAs, including SNPs. The robust activity of the enzyme and its affinity for RNA-splinted DNA substrates (apparent Km = 1 nM) enable sub-nanomolar detection of unique RNA species within a complex mixture, making SplintR ligase a superior choice for demanding RNA detection technologies. The activity is enhanced at higher temperatures (up to 37°C), and by supplementation with 5 mM Mn2+. The reaction is inhibited by salt concentrations in excess of 100 mM. The enzyme tolerates all base pair combinations at the ligation junction, but is partially inhibited by dC/G and dG/C base pairs at the donor (phosphorylated) side ligation junction, particularly when the +2 base was also a C/G base pair.
New England Biolabs, Inc. Bacteroides Heparinase II – 80 units
Bacteroides Heparinase II (also called Heparin Lyase II) is cloned from Bacteroides eggerthii. It is a low specificity enzyme that is active on both heparin and heparan sulfate. Bacteroides Heparinase II cleaves the glycosidic bond between N-sulfated and glucuronic or iduronic acid residues. The reaction yields oligosaccharide products containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. When used alone this enzyme rarely yields complete depolymerization of a polysaccharide chain, however disaccharide analysis is enhanced when used in combination with Heparinase I and III.