Modifying Enzymes
Worthington Biochemical Corporation DEOXYRIBONUCLEASE I RN 100UN
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied as a solution at approximately 2 Kunitz units per microliter (approximately 1 mg/ml) containing 50% glycerol and 1 mM calcium chloride. 9003-98-9
New England Biolabs, Inc. Bacteroides Heparinase III – 14 units
Bacteroides Heparinase III, also called Heparin Lyase III, is active on both heparin and heparan sulfate. Bacteroides Heparinase III can cleave the glycosidic bond between hexosamines and either iduronic acid or glucuronic acid residues. It is active in the presence of 6-sulfation. The reaction yields oligosaccharide products containing unsaturated uronic acids, which can be detected by UV spectroscopy at 232 nm
New England Biolabs, Inc. T7 DNA Ligase – 750000 units
T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7. It will catalyze the formation of a phosphodiester bond between adjacent 5' phosphate and 3' hydroxyl groups of duplex DNA. Cohesive end ligation and nick sealing can be efficiently catalyzed by T7 DNA Ligase. However, unlike T4 and T3 DNA Ligases, blunt end ligation is not efficiently catalyzed by T7 DNA Ligase. Addition of high concentrations of PEG 6000 [>= 20% (w/v)] to the reaction can force T7 DNA Ligase to have measurable activity. However, under typical reaction conditions blunt-end DNA ligation does not occur in the presence of T7 DNA Ligase, making it a good choice for applications in which blunt and cohesive ends of DNA are present but only the cohesive ends are to be joined. T7 DNA Ligase does not ligate blunt-end DNA fragments.
Worthington Biochemical Corporation STEMXYME 1 COLLAGENAS 50MG
A specialized combination of Animal Origin-Free Clostridium histolyticum collagenase (Code: CLSAFB) and animal origin free Bacillus polymyxa neutral protease with a minimum of 250 CLS units and 1,000 caseinase units per mg dry weight. Designed for stem cell and other primary cell isolations and bioprocessing applications where introduction of potential animal derived pathogens must be prevented. 2593-92-3
Abcam Recombinant human Catalase protein, 100UG
Recombinant human Catalase protein is a Human Full Length protein, in the 1 to 527 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Protein/ Peptide/ Enzyme Synonyms: Catalase, CAT
Conjugation: Unconjugated
The product is subject to the following: Abcam Restricted Use Statement
Roche Diagnostics Glucose Test kit for the Cedex Bio HT Analyze
Test kit for the Cedex Bio HT Analyzer. For use in quality control / manufacturing process only. Test kit for specific determination of this analyte. Kit includes 800 tests.
New England Biolabs, Inc. Thermostable 5' App DNA/RNA Ligase – 50 reactions
Thermostable 5' App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum. This enzyme is ATP independent. It requires a 5' pre-adenylated linker for ligation to the 3' -OH end of either RNA or single stranded DNA (ssDNA). The enzyme is also active in ligation of RNA with 2' -O-methylated 3 end to 5-adenylated linkers. The optimal temperature for ligation reaction is 60-65°C. The mutant ligase is unable to adenylate the 5' -phosphate of RNA or ssDNA, which reduces the formation of undesired ligation products (concatemers and circles). The ability of the ligase to function at 65°C might reduce the constraints of RNA secondary structure in RNA ligation experiments.
New England Biolabs, Inc. Bacteroides Heparinase I – 240 units
Bacteroides Heparinase I, also called Heparin Lyase I, is active on heparin and the highly sulfated domains of heparan sulfate. The reaction yields oligosaccharide products containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.
New England Biolabs, Inc. PNGase F, Recombinant – 15000 units
PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F, Recombinant is a recombinant amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
Sigma Aldrich Fine Chemicals Biosciences Deoxyribonuclease I from bovine pancreas Type IV, lyophilized powder, >=2,000 Kunitz units/mg protein | 9003-98-9 | MFCD00130918 | 375KU
Deoxyribonuclease I from bovine pancreas Type IV, lyophilized powder, >=2,000 Kunitz units/mg protein | 9003-98-9 | MFCD00130918 | 375KU
Cayman Chemical Oleoyl SerInol 50mg
An analog of ceramide and a GPR119 agonist (EC50 = 12 µM); induces cell death in F-11 murine neuroblastoma and U-87 human astrocytoma cells at 100 µM; induces apoptosis in rapidly dividing cells and prevents teratoma formation in a neonatal mouse engraftment model of teratoma formation
Cayman Chemical MaLNyl Coenzyme AlIthIM1mg
A CoA derivative used in fatty acid and polyketide synthesis and in the transport of α-ketoglutarate across the mitochondrial membrane; may mediate cancer cell cytotoxicity induced by fatty acid synthase inhibition
Apexbio Technology LLC Lambda Exonuclease(Synonyms: Lambda Exo, Lambda exonuclease enzyme, λ exonuclease, λ exonuclease enzyme), 5000U.
Lambda Exonuclease is a DNA-specific exonuclease enzyme that progressively degrades double-stranded DNA in a 5 3 direction preferentially initiating digestion at 5 -phosphorylated termini It displays minor activity toward single-stranded DNA and double-stranded DNA with non-phosphorylated ends but cannot start cleavage at internal nicks or gaps Lambda Exonuclease is commonly employed in molecular biology laboratories for the generation of single-stranded DNA templates DNA single-strand conformation polymorphism (SSCP) analysis selective degradation of linear plasmid forms while preserving supercoiled structures and rolling-circle amplification protocols
Medchemexpress LLC Diastase, Aspergillus oryzae | 9000-92-4 | 10 G
Diastase is an amylase from Aspergillus oryzae that hydrolyzes starch into sugars.
- Enzyme activity: ≥100000 U/g
- Unit definition: One unit of enzyme activity is defined as the amount of enzyme that can liquefy 1 g of soluble at 40°C and pH 5.0 in 1 hour.
- For research use only.
- We do not sell to patients.