Modifying Enzymes
STEMCELL Technologies Dispase, ACF, Size: 10 mg
Dispase, Animal Component-Free (ACF) is a neutral, amino-endoprotease that cleaves the N-terminal peptide bond of non-polar amino acid residues and is obtained from cultures free of animal-derived materials. Dispase has mild proteolytic activity, which makes it especially useful for the isolation and passaging of primary cells. Its proteolytic activity also allows it to maintain membrane integrity. Dispase is commonly used with other proteases such as collagenase in cell isolation and tissue dissociation for neural tissue (Dietrich et al.), kidney (Presnell et al.), epithelial tissue (Smoot et al.), endothelial tissue (Müller et al.), lung (Barkauskas et al.), colon (Roig et al.), and stem cells (Thomson et al.; Salmon et al.).
ABclonal Technology RNase H
RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.
New England Biolabs, Inc. yDcpS – 4000 units
yDcpS is the scavenging decapping enzyme from S. cerevisiae that hydrolyzes the phosphodiester bond between the gamma and beta phosphates of m7G-capped mRNA, producing diphosphorylated 5 ends and m7GMP. yDcpS is capable of decapping mRNAs of various lengths, decaps Cap0 and Cap1 with equal efficiency, and the diphosphorylated 5 end it produces is suitable for recapping with Vaccinia Capping Enzyme.
C LECTA GMBH DENARASE High Salt 5000 kU Enzymes & Nucleases
The salt-tolerant enzyme cleaves all forms of DNA and RNA and enables a simpler more efficient downstream process at salt concentrations above 150 mM - Purity 98% - Activity 250 U/ul - Formulation buffered aqueous glycerol solution - Storage Temperature -20C - Produced under ISO 9001 standard- Manufactured without the use of antibiotics Triton X-100 and raw materials of animal origin- - Net price excluding VAT and shipment costs
c-LEcta will ship the Products, as the case may be and at its sole discretion, either directly from Leipzig, Germany, or from c-LEcta’s third party service provider in the US. In the latter case, c-LEcta has already incurred all costs and fees related to the import of the Products into the US. Such costs and fees will be passed on by c-LEcta to the customer without any deductions or surcharges, if the Products are shipped from c-LEcta’s third party service provider in the US to the customer
C LECTA GMBH DENARASE 1000 kU, R&D Recombinant Endonucleases
For process development of biologicals such as viral vectors and vaccines The enzyme cleaves all forms of DNA and RNA and enables a simpler more efficient downstream process - Purity 99% - Activity 250 U/ul - Formulation buffered aqueous glycerol solution- Storage -20C- Produced in conformity with ISO 9001 standard- Manufacturing free of animal-derived raw materials and antibiotics- Depending on your application 10-100 U/ml is needed - Net price excluding VAT and shipment costs
c-LEcta will ship the Products, as the case may be and at its sole discretion, either directly from Leipzig, Germany, or from c-LEcta’s third party service provider in the US. In the latter case, c-LEcta has already incurred all costs and fees related to the import of the Products into the US. Such costs and fees will be passed on by c-LEcta to the customer without any deductions or surcharges, if the Products are shipped from c-LEcta’s third party service provider in the US to the customer
Gold Biotechnology Inc Proteinase K Solution 20 mg/ml 2 mL
Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.
New England Biolabs, Inc. Immobilized T4 DNA Ligase - 1.1 mg
Immobilized T4 DNA Ligase (IM-T4 DNA Ligase) is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg/ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry. Following a reaction, enzyme can be removed using a magnet thereby avoiding heat inactivation, and can be re-used.
Worthington Biochemical Corporation ELASTASE 25 MG
Elastase, Suspension, Code: ES, 2X Crystallized, Supplied as an aqueous suspension, Suitable for the Isolation of Type II lung cells, Store at 2 deg. to 8 deg.C, The diluted enzyme should be 0.22u filtered before use, 25mg
LGC GENOMICS LLC (Lucigen) RNase A, 2 mL @ 5 mg/mLEfficiently degrade unwanted RNA in DNA preparations and other samples.
This reagent is designed for use with the MasterPure™ kit protocols only.
Sigma Aldrich Fine Chemicals Biosciences Catalase from bovine liver aqueous solution, >=30,000 units/mg protein | 9001-05-2 | MFCD00081483 | 50MG
Catalase from bovine liver aqueous solution, >=30,000 units/mg protein | 9001-05-2 | MFCD00081483 | 50MG
Supelco Inc HYALURONIDASE TYPE 1V-S
Hyaluronidase, Type IV-S, lyophilized powder (essentially salt-free), 750-3000A units/mg solid
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC3999297 ENTEROKINASE ENTEROPEPTIDASE
World Precision Instrument Sensor 100U NO, 2PK Custom L
ISO-NOPF electrodes are available in 100 µm, 200 µm and 500 µm diameters. Utilizing the latest advances in nano-technology and material science, scientists at WPI’s Sensor Laboratory have created these completely flexible and virtually unbreakable NO sensors. The new sensors are based on a composite graphite NO-sensing element combined with a reference electrode. The surface of the sensor is then coated with a unique multi-layered NO-selective membrane.
Sigma Aldrich Fine Chemicals Biosciences alpha-Amylase Bacillu250ML
Amylase belongs to glycosyl hydrolase family 13. It comprises of A B and C domains. Domain A has two aspartic acid and a glutamic acid as catalytic residues. Domain B possesses Ca2 and Na binding sites.