Modifying Enzymes
New England Biolabs, Inc. Klenow Fragment (3'-5' exo-) – 200 units
Klenow Fragment (3' to 5' exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5' to 3' exonuclease activity and has mutations (D355A, E357A) which abolish the 3' to 5' exonuclease activity.
INTACT GENOMICS INC Cas9 Nuclease 40 µg
Intact Genomics Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR/Cas9-mediated genome editing (1, 2). The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie blue staining. Product Source:E. coli BL21 (DE3) strain expressing a Cas9 gene from Streptococcus pyogenes with an N-terminal 6xHis tag and C-terminal SV40 nuclear localization signal (NLS).Product Includes: Cas9 Nuclease 10x Cas9 Nuclease Reaction Buffer
LGC GENOMICS LLC (Lucigen) Poly(A) Polymerase Tailing Kit, 50 RxnsAdd poly(A) tails to the 3' ends of RNA molecules, including in vitro transcribed RNAs, with this robust kit.
The Poly(A) Polymerase Tailing Kit provides the enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3 end of any RNA. Poly(A) Polymerase is encoded by an E. coli gene that has been cloned into a plasmid and overexpressed in an E. coli strain.
Apexbio Technology LLC EZ Cap OVA mRNA (m1Ψ) 5x1mg (1mg/mL)
Ovalbumin (OVA) mRNA encodes a egg white glycoprotein with a molecular weight of approximately 45 000 daltons It can induce moderate immunogenicity in organisms and is commonly used as an immunogen in immunological experiments such as establishing animal models for hypersensitivity asthma etc EZ Cap OVA mRNA (m1 ) is provided at 1 mg/mL capped by co-transcriptional capping with a Cap 1 structure and polyadenylated Modified m1 incorporation reduces the host cell immune response and enhances mRNA stability and translation efficiency
Sigma Aldrich Fine Chemicals Biosciences beta-(1->3,4,6)-Galactosidase, positionally specific recombinant, expressed in E. coli, buffered aqueous solution | 9031-11-2 | MFCD01092682 | 1VL
beta-(1->3,4,6)-Galactosidase, positionally specific recombinant, expressed in E. coli, buffered aqueous solution | 9031-11-2 | MFCD01092682 | 1VL
Sigma Aldrich Fine Chemicals Biosciences beta(1->4)-Galactosidase, positionally specific from Streptococcus pneumoniae recombinant, expressed in E. coli, buffered aqueous solution | 9031-11-2 | MFCD01092684 | 1VL
beta(1->4)-Galactosidase, positionally specific from Streptococcus pneumoniae recombinant, expressed in E. coli, buffered aqueous solution | 9031-11-2 | MFCD01092684 | 1VL
Cayman Chemical ANTIBODY KU-0058948 10mg
A PARP1 and PARP2 inhibitor (IC50s = 3.4 and 1.5 nM, respectively); selective for PARP1 over PARP3, PARP4, and tankyrase (IC50s = 40, 1,200, and >10,000 nM, respectively); selectively inhibits the survival of embryonic stem cells lacking Brca1 or Brca2 over embryonic stem cells expressing wild-type Brca1 or Brca2; decreases the survival and induces apoptosis in P39 and MUTZ-3 AML cells at 1 µM; potentiates MS-275-induced cytotoxicity in patient-derived AML cells at 5 nM; reduces survival in PTEN-deficient HCT116 cells but not those expressing wild-type PTEN or HCT116+/neo
Bioss KD-Validated Coenzyme A syntha
Coenzyme A Synthase Primary Antibody Unconjugated Public Immunogen Range 530-564/564 Host Species Rabbit Target Species Human Mouse Rat Clonality Monoclonal Applications WB FCM IC Concentration Lot dependent Size 50 ul Storage Buffer Supplied in PBS (pH 7 4) containing 50% glycerol and 0 02% sodium azide
New England Biolabs, Inc. T4 DNA Ligase – 20,000 units – 400,000 units/ml
The industry standard for performance and quality. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA
- Joins blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA
- T4 DNA Ligase variants: Salt-T4, Hi-T4, Instant Sticky-end Ligase MM, Blunt/TA Ligase MM
Supply Solutions Liberase™ DH Research Grade, high Dispase concentration, 2x5mg
The most commonly used method for decellularization procedures, such as tissue dissociation and cell harvesting, is based on the use of proteolytic enzymes. Proteases disrupt the extracellular matrix of tissue to allow the release of individual cells or the harvesting of cultured cells for transfer. The goal of cell isolation is to maximize the yield of dissociated cells that are viable and functionally active.
ABclonal Technology PNGase F, Recombinant (Expressed in E.coli)
Peptide N-glycosidase F (PNGase F) is a glycosylpeptidase with a theoretical molecular weight of 35.9 kD that can cleave asparagine-linked high mannose, hybrid, and complex oligosaccharides from glycoproteins. PNGase F cleaves the amide bond between innermost N-acetylglucosamine (GlcNAc) and asparagine residues of the glycoprotein, converting asparagine to aspartic acid in the process. This product is commonly used for the deglycosylation of antibodies and glycoproteins.
Worthington Biochemical Corporation Trypsin Inhibitor, Soybean, Purified, 100mg
Source: Soybean. Animal Free/AF. Chromatographically purified as described by Frattali, V., and Steiner, R.: Biochem., 7, 521 (1968), modified by Worthington. A dialyzed, lyophilized powder. Purity >90% checked using SDS PAGE. Store at 2-8°C.
Supelco Inc LYSOZYME CHICKEN EGG WHITE 10G
Lysozyme, lyophilized powder, protein A%Y90A %, A%Y40,000A units/mg protein
AG Scientific Inc RNase A, 500 MG
RNase stands for ribonuclease, an RNA digesting enzyme. RNase consists of 124 amino acids in one polypeptide chain with 4 disulphide linkages. Ribonucleases can be divided into endoribonucleases and exoribonucleases. RNases have important roles in RNA degradation and turnover in all organisms. Ribonuclease enzyme can unwind the RNA helix by complexing with single-stranded RNA. RNase A is an endoribonuclease with functions in RNA metabolism and regulation of gene expression. It was found to play roles in diseases such as autoimmune diseases, renal insufficiencies and pancreas disorder.CAS Number: 9001-99-4Chemical Formula: C9H14N4O3Storage Temperature: -20CResearch or further manufacturing use only, not for food or drug use.
ABclonal Technology Endonuclease IV
Endonuclease IV derived from E. coli recognizes the depurine/depyrimidine (AP) site on the double- stranded DNA molecule, and cleaves the first phosphodiester bond at the 5 ?end of the AP site to form 3-OH and 5dRP . In addition, Endonuclease IV has 3?diesterase activity, which releases phosphoglyceraldehyde, intact deoxyribose 5 -phosphate, and phosphoric acid from the 3 ?end of DNA. The best substrate for this enzyme is AP double-stranded DNA, but it is also active against AP single-stranded DNA.