Modifying Enzymes
Enzo Life Sciences Matrix Metalloproteinase-9 (MMP-9) fluorometric drug discovery kit (1x96wells)
A QUANTIZYME Assay System. The MMP-9 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-9 (MMP-9, gelatinase B) using a quenched fluorogenic peptide: OMNIMMP fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-9, a potential therapeutic target. Included are active enzyme, assay buffer, a prototypic control inhibitor (NNGH)3, and a detailed instruction booklet. Alternative Name: Gelatinase B, 92kDa Type IV collagenase. Applications: Fluorescent detection, HTS, Activity assay. Contents: MMP-9 enzyme (human) (recombinant), substrate, calibration standard, inhibitor, assay buffer, 96-well white microplate.
Sigma Aldrich Fine Chemicals Biosciences Creatine Kinase (CK), 100 mg
Creatine kinase has been used as a component of translation buffer used in in vitro translation assay and as a component of energy regeneration mix.
FUJIFILM BIOSCIENCES INC ACHROMOPEPTIDASE 1G
*Orders for this supplier are non-cancellable/non-returnable once they are processed.* Achromopeptidase 1g. 78642-25-8
Sigma Aldrich Fine Chemicals Biosciences Alpha-Amylase from porcine pancreas | MFCD00081319 | 1MU
Alpha-Amylase from porcine pancreas | MFCD00081319 | 1MU
New England Biolabs, Inc. EnGen Spy Cas9 HF1 - 2500 pmol
EnGen Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen Spy Cas9 NLS from Streptococcus pyogenes with reduced non-specific DNA cleavage. Spy Cas9 is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The single guide RNA (sgRNA) targets Cas9 to the region immediately upstream of a 5'-NGG-3' protospacer adjacent motif (PAM) producing a double stranded break 3 bases upstream of the PAM. EnGen Spy Cas9 HF1 contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C-termini of the protein.
ACROBiosystems GENIUS™Nuclease DMF Filed
GENIUS™Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-8 bases in length.
Sigma Aldrich Fine Chemicals Biosciences alpha-Amylase Bacillu250ML
Amylase belongs to glycosyl hydrolase family 13. It comprises of A B and C domains. Domain A has two aspartic acid and a glutamic acid as catalytic residues. Domain B possesses Ca2 and Na binding sites.
Cayman Chemical 2-Oleoyl Glycerol 5mg
A natural monoacylglycerol that is metabolized by monoacylglycerol lipase; an agonist of GPR119 (EC50 = 2.5 µM), stimulating the release of glucagon-like peptide-1 from intestinal L-cells
Diagenode Inc Tagmentase TN5 TRN Unloaded, 10 μl, Hyperactive Tn5 transposase, Mg++ dependent, 19-bp ME DNA sequence required, 2 mg/ml protein concentration, 53.3 kDa molecular weight
Diagenode Tagmentase (Tn5 transposase) is a hyperactive enzyme designed for a wide range of molecular biology applications. It enables an efficient "cut and paste" tagmentation reaction, allowing simultaneous DNA cleavage and the insertion of specific oligonucleotides or sequencing adapters. As an unloaded variant, it provides researchers with the flexibility to customize the enzyme with oligonucleotides tailored to their specific experimental needs. This Tn5 transposase is essential for various Next-Generation Sequencing workflows.
- Enzyme type: Hyperactive Tn5 transposase
- Loading status: Unloaded
- Function: Catalyzes "cut and paste" tagmentation
- Activity requirement: Mg++ dependent
- Optimal pH: 7.5-8.0
- Optimal temperature: 37-55 °C
- Storage conditions: Store at -20 °C
- Protein concentration: 2 mg/ml
- Volume: 10 μl
Biotium Double Stranded DNA (dsDNA)(121-3), CF594 conjugate, 0.1mg/mL
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.,Deoxyribonucleic acid (DNA) is a long polymer of nucleotides that is held together by a backbone made of sugars and phosphate groups. It holds the genetic instructions for the development and function of living things. DNA is crucial for living organisms, and all known cellular life and some viruses contain DNA. In eukaryotes, DNA exists in the cell nucleus, while in prokaryotes; DNA is located in the cytoplasm. In living organisms, DNA does not usually exist as a
New England Biolabs, Inc. EcoRI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G^AATT_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Abcam Recombinant Human Liver Carboxylesterase 1/CES1 protein, 10UG
Recombinant Human Liver Carboxylesterase 1/CES1 protein is a Human Fragment protein, in the 19 to 562 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.
Protein/ Peptide/ Enzyme Synonyms: CES2, SES1, CES1, Liver carboxylesterase 1, Acyl-coenzyme A:cholesterol acyltransferase, Brain carboxylesterase hBr1, Carboxylesterase 1, Cholesteryl ester hydrolase, Cocaine carboxylesterase, Egasyn, HMSE, Methylumbelliferyl-acetate deacetylase 1, Monocyte/macrophage serine esterase, Retinyl ester hydrolase, Serine esterase 1, Triacylglycerol hydrolase, ACAT, CE-1, hCE-1, CEH, REH, TGH
Conjugation: Unconjugated
The product is subject to the following: Abcam Restricted Use Statement
Agilent Technologies Glyko PNGase F (native), 100 mU
Native PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. PNGase F releases intact N-linked oligosaccharides from glycoproteins and glycopeptides. The enzyme will not cleave oligosaccharides from a single asparagine residue and cleavage does not occur if there is core α1-3 linked fucose as commonly encountered in plant glycoproteins Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage.