Modifying Enzymes
Worthington Biochemical Corporation Trypsin Vial, NCIS, 5 vials
A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 2 ml of HBSS yields a solution of 500µg/ml of trypsin, Code: TRLS. Suitable for cell isolation and culture applications. Store at 2-8°C.
Cayman Chemical Coenzyme Q10-d6 1mg
An internal standard for the quantification of CoQ10 by GC- or LC-MS
Medchemexpress LLC Alpha-amylase, porcine pancreatic | 9000-90-2 | 100 KU
α-Amylase, Porcine Pancreatic (1,4-alpha-D-Glucan-glucanohydrolase) is a hydrolase enzyme isolated from porcine pancreatic. It catalyzes the hydrolysis of internal α-1, 4-glycosidic linkages in starch to yield products like glucose and maltose. This product is intended for life science research.
- Can be used in the study of starch metabolism.
- Shows binding activity toward N-linked oligosaccharides of glycoproteins, a unique feature not shared by α-amylases from saliva, wheat, or fungus.
- Enhances the release of Dexamethasone from its γ-cyclodextrin complex at relatively low concentrations of γCD by catalyzing the hydrolysis of γCD.
Sigma Aldrich Fine Chemicals Biosciences Hyaluronidase from sheep testes Type II, lyophilized powder, >=300 units/mg solid | 37326-33-3 | MFCD00131351 | 100MG
Hyaluronidase from sheep testes Type II, lyophilized powder, >=300 units/mg solid | 37326-33-3 | MFCD00131351 | 100MG
Biotang Inc Carboxypeptidase-B Rat Recombinant, Carboxypeptidase B Rat,Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a Mw of 35kDa is purified by standard chromatography techniques.
Carboxypeptidase-B Rat Recombinant, Carboxypeptidase B Rat,Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a Mw of 35kDa is purified by standard chromatography techniques.
Sigma Aldrich Fine Chemicals Biosciences Isovaleryl coenzyme A lith10MG
Isovaleryl coenzyme A an intermediate of the leucine catabolism. It is synthesized from -methylbutyric acid by the action of enzyme acyl CoA synthetase. The conversion of isovaleryl-CoA to methylcrotonyl-CoA is catalyzed by the enzyme isovaleryl-CoA dehydrogenase in leucine catabolism pathway. The enzyme isovaleryl-CoA oxidase also calalyzes this conversion in fatty acid -oxidation pathway.
Diagenode Inc Tagmentase TN5 TRN Unloaded, 10 μl, Hyperactive Tn5 transposase, Mg++ dependent, 19-bp ME DNA sequence required, 2 mg/ml protein concentration, 53.3 kDa molecular weight
Diagenode Tagmentase (Tn5 transposase) is a hyperactive enzyme designed for a wide range of molecular biology applications. It enables an efficient "cut and paste" tagmentation reaction, allowing simultaneous DNA cleavage and the insertion of specific oligonucleotides or sequencing adapters. As an unloaded variant, it provides researchers with the flexibility to customize the enzyme with oligonucleotides tailored to their specific experimental needs. This Tn5 transposase is essential for various Next-Generation Sequencing workflows.
- Enzyme type: Hyperactive Tn5 transposase
- Loading status: Unloaded
- Function: Catalyzes "cut and paste" tagmentation
- Activity requirement: Mg++ dependent
- Optimal pH: 7.5-8.0
- Optimal temperature: 37-55 °C
- Storage conditions: Store at -20 °C
- Protein concentration: 2 mg/ml
- Volume: 10 μl
Sigma Aldrich Fine Chemicals Biosciences N Glycosidase f PNGase f o
Peptide-N-glycosidase f Peptide-N4-(acetyl-beta-glucosaminyl)-asparagine amidase
Sigma Aldrich Fine Chemicals Biosciences DNA Ligase from T4-infected Escherichia coli buffered aqueous glycerol solution | 9015-85-4 | MFCD00163508 | 100UN
DNA Ligase from T4-infected Escherichia coli buffered aqueous glycerol solution | 9015-85-4 | MFCD00163508 | 100UN
Worthington Biochemical Corporation DEOXYRIBONUCLEASE 1
Recombinant Bovine pancreatic deoxyribonuclease 1 produced in Pichia pastoris. Chromatographically purified. Free of animal derived components, RNases & proteases. A liquid preparation in 5mM Calcium Acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol.
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, >=2,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 2KU
Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, >=2,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 2KU
AURORA BIOLABS LLC Enterokinase (EK) Activity Assay Kit,96 rxn
The Enterokinase (EK) Activity Assay Kit is a fluorogenic assay designed to quantitatively measure EK protease activity. The kit includes a specific EK substrate (Asp-Asp-Asp-Asp-Lys) conjugated to the fluorophore AFC (7-amino-4-trifluoromethylcoumarin). Upon proteolytic cleavage of the substrate by EK, free AFC is released, generating a strong fluorescent signal. The fluorescence can be detected using a microplate fluorescence reader with excitation at 365 nm and emission at 505 nm. The increase in fluorescence intensity is directly proportional to EK enzymatic activity and can be used to calculate protease activity. Purified EK protease is provided in the kit as a positive control to validate assay performance and ensure proper assay setup.
STEMCELL Technologies Dispase, ACF, Size: 10 mg
Dispase, Animal Component-Free (ACF) is a neutral, amino-endoprotease that cleaves the N-terminal peptide bond of non-polar amino acid residues and is obtained from cultures free of animal-derived materials. Dispase has mild proteolytic activity, which makes it especially useful for the isolation and passaging of primary cells. Its proteolytic activity also allows it to maintain membrane integrity. Dispase is commonly used with other proteases such as collagenase in cell isolation and tissue dissociation for neural tissue (Dietrich et al.), kidney (Presnell et al.), epithelial tissue (Smoot et al.), endothelial tissue (Müller et al.), lung (Barkauskas et al.), colon (Roig et al.), and stem cells (Thomson et al.; Salmon et al.).
Enzo Life Sciences MMP-2 proenzyme (human fibroblasts) (5µg)
Alternative name: Matrix metalloproteinase 2, Gelatinase A, 72 kDa Type IV collagenase. Purity: ≥90% (SDS-PAGE, Western blot). MW: ~72kDa. Formulation: Liquid. In 50mM TRIS-HCl, pH 7.0, containing 200mM NaCl, 5mM CaCl2, 1µM ZnCl2, 0.05% BRIJ 35 and 0.05% sodium azide. Source: Isolated from human rheumatoid synovial fibroblasts. Requires activation. Long Term Storage: -80°C. Handling: Avoid freeze/thaw cycles.
Enzo Life Sciences MMP-10 (catalytic domain) (human), (recombinant) (10 µg)
Alternative name: Matrix metalloproteinase 10, Stromelysin 2. Formulation: Liquid. In 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20µM ZnCl2, 0.5% Brij-35, and 30% glycerol. Source: Produced in E. coli. Active Matrix Metalloproteinase-10 (MMP-10, stromelysin-2, transin-2) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-10 (Phe99-Glu271, NM_2425) with a C-terminal purification tag. This comprises an active form of MMP-10 which lacks the C-terminal hemopexin domain. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected. Long Term Storage: -80°C.