Modifying Enzymes
Supply Solutions Sigma Aldrich - Phosphatase,alkaline(AP),EIA Grade,15 mg * Third Party Item
Sigma Aldrich - Phosphatase,alkaline(AP),EIA Grade,15 mg * Third Party Item
New England Biolabs, Inc. Bst DNA Polymerase, Large Fragment – 8000 units
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5' to 3' polymerase activity, but lacks 5' to 3' exonuclease activity.
New England Biolabs, Inc. Cre Recombinase – 50 units
Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. The enzyme requires no energy cofactors and Cre-mediated recombination quickly reaches equilibrium between substrate and reaction products. The loxP recognition element is a 34 base pair (bp) sequence comprised of two 13 bp inverted repeats flanking an 8 bp spacer region which confers directionality. Recombination products depend on the location and relative orientation of the loxP sites. Two DNA species containing single loxP sites will be fused. DNA between directly repeated loxP sites will be excised in circular form while DNA between opposing loxP sites will be inverted with respect to external sequences.
New England Biolabs, Inc. β-N-Acetylhexosaminidase f - 500 units
b-N-Acetylhexosaminidasef is an exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing b-N-Acetylgalactosamine and glucosamine residues from oligosaccharides.
Sigma Aldrich Fine Chemicals Biosciences Lipoprotein Lipase from bovine milk ammonium sulfate suspension, 2,000units/mg protein (BCA), 9004-02-8, MFCD00131523
Lipoprotein Lipase from bovine milk ammonium sulfate suspension, 2,000units/mg protein (BCA)
New England Biolabs, Inc. EcoRV – 4000 units
A restriction endonuclease that recognizes the sequence GAT_^ATC.
New England Biolabs, Inc. Fpg – 2500 units
Fpg (formamidopyrimidine [fapy]-DNA glycosylase) (also known as 8-oxoguanine DNA glycosylase) acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves both 3' and 5' to the AP site thereby removing the AP site and leaving a 1 base gap. Some of the damaged bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil.
New England Biolabs, Inc. Enterokinase, light chain – 480 units
Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.
New England Biolabs, Inc. MseI – 2500 units
A restriction endonuclease that recognizes the sequence T^TA_A.
New England Biolabs, Inc. Mung Bean Nuclease – 1500 units
Mung Bean Nuclease is a single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions leaving blunt, ligatable ends.
ELASTIN PRODUCTS COMPANY INC HU SPUTUM LEUCOCYTE ELAST 1MG
Human Sputum Leucocyte Enzyme elastase 1mg
FUJIFILM BIOSCIENCES INC NUCLEASE P1
*Orders for this supplier are non-cancellable/non-returnable once they are processed.*
New England Biolabs, Inc. Taq DNA Ligase Reaction Buffer – 6 ml
This is the NEB reaction buffer supplied with Taq DNA Ligase. Taq DNA Ligase requires NAD+ as a cofactor which is incorporated into the buffer. New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
New England Biolabs, Inc. Klenow Fragment (3'-5' exo-) – 200 units
Klenow Fragment (3' to 5' exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5' to 3' exonuclease activity and has mutations (D355A, E357A) which abolish the 3' to 5' exonuclease activity.