Modifying Enzymes
New England Biolabs, Inc. EnGen Lba Cas12a (Cpf1) – 70 pmol
EnGen Lba Cas12a from Lachnospiraceae bacterium ND2006 is a site-specific DNA endonuclease guided by a single 41-44 nucleotide guide RNA (gRNA) . Targeting requires a gRNA complementary to the target site as well as a 5' TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen Lba Cas12a occurs ~18 bases 3' of the PAM and leaves 5' overhanging ends. EnGen Lba Cas12a has Simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N and C-termini of the protein.
Sigma Aldrich Fine Chemicals Biosciences beta-Glucuronidase from Helix pomatia Type H-5, lyophilized powder, >=400,000 units/g solid | 9001-45-0 | MFCD00130629 | 500KU
beta-Glucuronidase from Helix pomatia Type H-5, lyophilized powder, >=400,000 units/g solid | 9001-45-0 | MFCD00130629 | 500KU
New England Biolabs, Inc. Thermostable 5' App DNA/RNA Ligase – 50 reactions
Thermostable 5' App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum. This enzyme is ATP independent. It requires a 5' pre-adenylated linker for ligation to the 3' -OH end of either RNA or single stranded DNA (ssDNA). The enzyme is also active in ligation of RNA with 2' -O-methylated 3 end to 5-adenylated linkers. The optimal temperature for ligation reaction is 60-65°C. The mutant ligase is unable to adenylate the 5' -phosphate of RNA or ssDNA, which reduces the formation of undesired ligation products (concatemers and circles). The ability of the ligase to function at 65°C might reduce the constraints of RNA secondary structure in RNA ligation experiments.
Sigma Aldrich Fine Chemicals Biosciences β-Galactosidase from Escherichia coli | 9031-11-2 | MFCD00130623 | 1ku
β-Galactosidase from Escherichia coli | 9031-11-2 | MFCD00130623 | 1ku
Sigma Aldrich Fine Chemicals Biosciences Deoxyribonuclease I from bovine pancreas Type II, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein | 9003-98-9 | MFCD00130918 | 40ku
Deoxyribonuclease I from bovine pancreas Type II, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein | 9003-98-9 | MFCD00130918 | 40ku
New England Biolabs, Inc. EarI – 2500 units
A restriction endonuclease that recognizes the sequence CTCTTCN^NNN_.
New England Biolabs, Inc. Factor Xa Protease – 50 µg
Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-Glu/Asp-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. The most common secondary site, among those that have been sequenced, is Gly-Arg.
New England Biolabs, Inc. PNGase F (Glycerol-free) – 15000 units
PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F (Glycerol-free) is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
- Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
- >= 95% purity, as determined by SDS-PAGE and intact ESI-MS
- Non-recombinant with no detectable endoglycosidase F1, F2 or F3 contamination
- Optimal activity and stability for up to 24 months
- Can be used under native or denaturing conditions
New England Biolabs, Inc. Endonuclease VIII – 1000 units
Endonuclease VIII from E. coli acts as both an N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves 3' and 5' to the AP site leaving a 5' phosphate and a 3' phosphate. Damaged bases recognized and removed by Endonuclease VIII include urea, 5, 6- dihydroxythymine, thymine glycol, 5-hydroxy-5- methylhydantoin, uracil glycol, 6-hydroxy-5, 6-dihydrothymine and methyltartronylurea. While Endonuclease VIII is similar to Endonuclease III, Endonuclease VIII has and lyase activity while Endonuclease III has only lyase activity.
Abcam Recombinant human Catalase protein, 100UG
Recombinant human Catalase protein is a Human Full Length protein, in the 1 to 527 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Protein/ Peptide/ Enzyme Synonyms: Catalase, CAT
Conjugation: Unconjugated
The product is subject to the following: Abcam Restricted Use Statement
American Research Products Inc ALANINE TRANSAMINASE KIT BA007
5000265320 ALANINE TRANSAMINASE KIT BA007
Biotium Double Stranded DNA (dsDNA)(DSD/958), 0.2mg/mL
This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Deoxyribonucleic acid (DNA) is a nucleic acid that stores long-term information regarding the development and function of all known living organisms. DNA consists of two long nucleotide polymers, which are composed of four bases, namely adenine, thymine, guanine and cytosine, all of which are flanked by a phosphate-deoxyribose backbone. Normally, DNA exists as a double-stranded (ds) molecule that forms in the shape of a double helix, allowing the bases and the backbone of the two strands to interact, thus forming a polynucleotide. When the double helix is unwound (either by enzymes or heat), DNA exists as a single-stranded (ss) molecule that is less stable than the double helix, but is necessary for protein acces
Sigma Aldrich Fine Chemicals Biosciences Peroxidase, Nitrospira sp., recombinant >=10.0 U/g | 9003-99-0 | 10MG
Peroxidase, Nitrospira sp., recombinant >=10.0 U/g | 9003-99-0 | 10MG
Biotium Double Stranded DNA (dsDNA)(121-3), Biotin conjugate, 0.1mg/mL
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.,Deoxyribonucleic acid (DNA) is a long polymer of nucleotides that is held together by a backbone made of sugars and phosphate groups. It holds the genetic instructions for the development and function of living things. DNA is crucial for living organisms, and all known cellular life and some viruses contain DNA. In eukaryotes, DNA exists in the cell nucleus, while in prokaryotes; DNA is located in the cytoplasm. In living organisms, DNA does not usually exist as a
Biotium Double Stranded DNA (dsDNA)(AE-2), CF488A conjugate, 0.1mg/mL
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.,Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds be