RNA Preparation and Purification
Norgen Biotek Corp Plasma/Serum RNA Purification Maxi Kit
This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a two column method. This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 2 mL to 5 mL. Plasma samples prepared from blood collected on heparin should not be used.The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL. All components for the purification and concentration are provided in one convenient and fast kit for the easy processing of large input volumes of bodily fluids.The purified plasma/serum RNA is fully compatible with all downstream applications including NGS, and more.
Omega Biotek Inc E.Z.N.A. HP Total RNA Kit, 200 preps, RNA binding capacity 100 μg (RNA >200 bases), silica-based spin column
The OMEGA BIOTEK E.Z.N.A. HP Total RNA Kit provides an efficient solution for the isolation of high-quality total RNA from eukaryotic cells and tissues. It employs a streamlined purification process, integrating sample homogenization and genomic DNA removal into a single step, utilizing advanced column technology. This kit enhances user safety by eliminating toxic organic solvents and simplifies the protocol for RNA purification, making it suitable for various sensitive applications.
- Rapid isolation in approximately 25 minutes for quick experimental turnaround
- High-performance RNA quality and yield
- Versatile compatibility with various cell and tissue types
- Safe and convenient with no toxic organic solvents
- Optimized for sensitive downstream applications like RT-PCR and next-generation sequencing
- High binding capacity of 100 μg for RNA >200 bases
Sigma Aldrich Fine Chemicals Biosciences RNA from yeast
RNA from yeast is the total RNA obtained from Saccharomyces cerevisiae.
Zymo Research Corporation Load N Go Quick-DNA/RNA HT Kit
The Load N' Go Quick-DNA/RNA HT kit offers a streamlined solution for high-throughput nucleic acid extraction from a variety of biological and clinical samples, such as swabs, biological liquids, and tissues. Compatible with robotic sample processors, this kit ensures the efficient isolation of high-quality DNA and RNA, making it suitable for sensitive downstream applications, including Next-Gen sequencing and RT/qPCR, ultimately enhancing the precision and reliability of scientific research.
Zymo Research Corporation Quick-DNA/RNA HT
TheQuick-DNA/RNAHTkit is intended for rapid, high-throughput nucleic acid extraction (automated or manual) from any biological or clinical sample (e.g., swabs – nasal/nasopharyngeal, oropharyngeal, etc.; biological liquids – blood, plasma, serum, saliva, sputum, cells in suspension, etc.; tissue – needle biopsies, LCM, etc.) and/or samples stored in most collection matrices and devices (e.g., UTM, VTM, *DNA/RNA Shield™, RNAlater, RNAProtect, etc.). The kit is compatible with robotic-type sample processors (i.e, bead movers) in combination with sensitive downstream molecular amplification assays. High-quality DNA/RNA extracted with theQuick-DNA/RNAHTkit can be used for Next-Gen sequencing, RT/qPCR and more.
Apexbio Technology LLC HyperScribe™ All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, UTP, T7, poly(A)), 25rxns
HyperScribe™ All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, UTP, T7, poly(A)) is designed for quick production of ARCA capped, 5mCTP and UTP modified, poly(A) tailed mRNA in vitro. Capped mRNA is synthesized by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA, Catalog No. B8175) using T7 RNA Polymerase. The capping of ARCA ensures high translation efficacy. The addition of 5mCTP (Catalog No. B7967) / UTP (Catalog No. B7972) can reduce host cell immune response. After a brief DNase I treatment to remove the template DNA, capped and modified mRNA is poly(A) tailed with Poly(A) Polymerase. Polyadenylation (i.e. addition of Poly(A) tail) plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation. The kit contains sufficient reagents to carry out 25 reactions, 20 l each time. Up to 25-180 g of RNA can be generated with 1 g of control template per standard reaction.
Norgen Biotek Corp Urne Cellfree Circ Rna Minikit
This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating RNA, including exosomal RNA as well as viral RNA from fresh, preserved or frozen urine samples from volumes ranging from 250 µL to 2 mL. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. The purified urine RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Cellscript Inc ScriptCap™ m⁷G Capping System, 25 reactions, Post-transcriptional 5' capping, ~100% Cap 0 capping efficiency
ScriptCap™ m⁷G Capping System, 25 reactions, Post-transcriptional 5' capping, ~100% Cap 0 capping efficiency