Protein Labeling Reagents
Biotium CF568 Biocytin
Fluorescent biocytin conjugates are cell-impermeant, fixable polar tracers with biotin and an aldehyde-fixable primary amine groups. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF568 dye has excitation/emission at 562/583 nm.
Biotium CF770R Succinimidyl Ester
CF Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red/near-IR fluorescent CF770R dye has excitation/emission at 770/701 nm.
Biotium 5-(AND-6)-CARBOXYTetramethylrhodamine SU
5(6)-TAMRA SE (full name: 5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester (mixed isomers)) is the amine reactive form of TAMRA, one of the most commonly used red fluorescent dyes. The succinimidyl ester reacts readily with primary or secondary amines under mild conditions. Properties: Ex/Em (MeOH) = 540/565 nm; e = 95,000; Red solid soluble in DMF or DMSO; Store at -20°C and protect from light; C 29 H 25 N 3 O 7; MW: 527.5; [150408-83-6]
Vector Laboratories NEUROBIOTIN Tracer
NEUROBIOTIN Tracer (SP-1120) is an amino derivative of biotin that can be used as an intracellular label for cells, particularly neurons. It is used for visualizing neural architecture and for the identification of gap junction coupling. Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells - Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading - Can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods
Vector Laboratories Biotin (Long Arm) Hydrazide, 50 mg
Specialty Products. Biotin (Long Arm) Hydrazide reacts with aldehyde groups and thus can be used to label carbohydrate groups present in a wide variety of macromolecules. The spacer arm in this product allows optimal accessibility of avidin or streptavidin conjugates to biotin. Incorporation of Biotin (Long Arm) Hydrazide involves a two-step process. First, the saccharide is treated with periodate (not included) that produces aldehyde groups on sugars containing unsubstituted vicinal hydroxyl groups (these are usually present at the non-reducing termini of the oligosaccharide). Next, Biotin (Long Arm) Hydrazide reacts with the aldehyde groups to produce a stable linkage between the saccharide and biotin. This reagent can also be used to couple biotin to carboxyl groups on proteins and other molecules using a carbodiimide coupling method, and to specifically label RNA with biotin at the 3' end.
Lumiprobe Sulfo-Cyanine3 maleimide | 1656990-68-9 | MFCD28385507 | 1 mg
Sulfo-Cyanine3 maleimide | Mol Wt: 776.969 | 1656990-68-9 | MFCD28385507 | 1 mg
![Aat Bioquest 5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-68-7*](https://assets.fishersci.com/TFS-Assets/CCG/product-images/default.jpg-250.jpg)
Aat Bioquest 5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-68-7*
The single TAMRA isomers are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purification that is often required in the conjugation processes.
Cayman Chemical BODIPY-PalmItate 1mg
Fluorescently tagged palmitic acid; ex/em = 488/508 nm, respectively; has been used to monitor fatty acid uptake and metabolism in cultured cells
Cayman Chemical BIotIn-Cholesterol 5mg
A biotinylated form of cholesterol; has been used in the synthesis of ergosterol peroxide derivatives; has been used in the synthesis of biotin-conjugated liposomes and micelles for drug delivery in vitro
Quanterix Inc TSA Plus TMR 50-150 Slides
TSA Plus tetramethylrhodamine (TMR) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra TMR in the localized area of the probe or antibody.
Biotium MemBrite™ Fix 488/515 Cell Surface Staining Kit, 100 Reactions
MemBrite™ Fix Cell Surface Staining Kits are designed for covalently staining the surface of live cells to conveniently visualize cell boundaries in immunofluorescence experiments. MemBrite™ Fix are new covalent cell surface stains that react irreversibly with cell surface proteins for staining that can withstand formaldehyde or alcohol fixation, and detergent permeabilization. Unlike lectins such as WGA, which bind specific targets that may vary between cell types, MemBrite™ Fix dyes react widely with cell surface proteins. MemBrite™ Fix staining is rapid and non-toxic to cells, and because MemBrite™ Fix dyes are highly water soluble, they stain cells much more evenly than traditional lipophilic membrane dyes like DiO, DiI, PKH, Vybrant, or CellMask™. This trial size kit has reagents sufficient for 100 labeling reactions. The kit contains 1 vial of MemBrite™ Fix 488/515 that is sufficient for 20 uL of 1000X dye after DMSO reconstitution.
Biotium Tyramide Amplification Kit with HRP Streptavidin and CF543 Tyramide
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. The kit contains HRP goat anti-mouse CF543 dye tyramide. The dye has an excitation/emission of 541/560 nm. The kit contains sufficient reagent for 50-150 slides.