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Reporter genes are attached to a regulatory sequence of a gene of interest. When expressed by the host organism, they generate measurable, easily identifiable characteristics that can be used as an indication of whether a gene has been taken up or expressed.
The Proteasome-Glo Assays are homogeneous, luminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome in a purified enzyme-based format (not suitable for cell-based applications).
Recombinant full-length human PKC gamma was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC gamma is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
Recombinant full-length human RSK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RSK2 is a member of the RSK (ribosomal S6 kinase) family that are growth factor-regulated serine-threonine kinases.
The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Recombinant human ROCK1 (amino acids 17-535) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ROCK1 is a ubiquitously expressed serine-threonine kinase that is a downstream target of the small GTPase RhoA.
Recombinant full-length human PKC alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC alpha is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Recombinant full-length human CDK2 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed.
Configured with NanoLuc Luciferase for use in reporter gene assays of transcriptional control—ideal for cloning of binding site or response element not in need of basic promoters
Recombinant human RON (amino acids 983-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RON is a macrophage stimulating 1 receptor c-met-related tyrosine kinase.
Recombinant human PDGFR alpha (amino acids 550-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PDGFR alpha is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity.