Gene Reporter Systems
Promega GSK3β Kinase Enzyme System
Recombinant full-length human GSK3 beta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. GSK3 beta is a serine-threonine protein kinase originally identified as the kinase that phosphorylates and inhibits glycogen synthase.
Promega PDGFRα Kinase Enzyme System
Recombinant human PDGFR alpha (amino acids 550-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PDGFR alpha is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity.
Promega GTPase-Glo Assay, 10,000 assays
The GTPase-Glo Assay assesses the activities of GTPases, GAPs and GEFs, which are components of the GTPase cycle, by detecting the amount of GTP remaining after GTP hydrolysis in a GTPase reaction.
Promega ADP-Glo™ Max ADP Detection Assay Kit
The ADP-Glo™ Max Assay is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure ATPase or kinase activity by quantifying the amount of ADP produced in a reaction.
Promega FcgammaRIIa-H ADCP Reporter Bioassay, Core Kit 5X, 1 each
The FcgammaRIIa-H ADCP Reporter Bioassay is a biologically relevant MOA-based assay that can be used to measure the potency and stability of antibodies and other biologics that specifically bind and activate FcgammaRIIa.
Promega Coelenterazines
We offer Coelenterazine (native) and Coelenterazine-h. Coelenterazine (native) is the luminescent substrate for Renilla luciferase and apoaequorin.
Promega β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer
The Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer is used to assay beta-galactosidase activity in cell lysates.
Promega PCR Optimization Kit, 1 each
PCR Optimization Kit is a portfolio of preformulated high-quality buffers, 25mM MgCl2 and GoTaq MDx Hot Start Polymerase. Perform a series of short experiments to quickly survey which combination will yield optimal PCR performance specific for your assay.
Promega ABL1 Kinase Enzyme System
Recombinant human ABL1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase implicated in processes of cell differentiation, cell division and others.
Promega Dual-Luciferase™ Reporter (DLR™) Assay Systems
The Dual-Luciferase Reporter (DLR) Assay System provides an efficient means of performing two reporter assays. In the DLR Assay, the activities of firefly and Renilla luciferases are measured sequentially from a single sample.
Promega FYN A Kinase Enzyme System
Full-length recombinant human FYN A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FYN A (Fyn isoform A) is a member of the SRC tyrosine kinase oncogene family showing high homology to YES1, FGR and SRC.
Promega RET Kinase Enzyme System
Recombinant human RET (amino acids 658-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RET gene codes for a transmembrane tyrosine kinase.
Promega mFcgammaRIV ADCC Reporter Bioassay, Core Kit, 1 each
The mFcgammaRIV ADCC Reporter Bioassay is a biologically relevant MOA-based assay that can be used to measure the activity of mouse antibodies that specifically bind and activate FcgammaRIV.
Promega Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay System
The Proteasome-Glo™ Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells.
Promega GloSensor™ cAMP Assay
The GloSensor cAMP Assay is based on a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. This live-cell assay excels at kinetic and modulation studies of signaling through cAMP.