General Cell Based Assays
Cayman Chemical SupRoxID DIsmutase asy KI 96
Cayman’s Superoxide Dismutase Assay Kit provides a convenient method of measuring SOD activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates. The assay relies on xanthine oxidase to produce superoxide radicals, which then reduce a tetrazolium salt to a formazan dye, detectable at 450 nm. SOD in the sample inhibits this reduction by catalyzing the dismutation of superoxide radicals. Thus, the degree of inhibition is directly proportional to SOD activity. The SOD Assay Kit measures all three types of SOD (Cu/Zn-, Mn-, and Fe-SOD). Mitochondrial Mn-SOD can be assayed separately following the procedure outlined in the kit booklet.
Cayman Chemical FFAR1GPR40 Reporter asy 1ea
Coriolis consumables ensure optimal collection efficiency for the Coriolis Air Sampler.
Cayman Chemical UrIc AcId asy KIt 96Wel
Cayman’s Uric Acid Assay Kit provides fluorescence- or colorimetric-based methods for detecting uric acid in plasma, serum, and urine. In the assay, uricase catalyzes the conversion of uric acid to allantoin, hydrogen peroxide (H2O2), and carbon dioxide. H2O2, in the presence of horseradish peroxidase, reacts stoichiometrically with ADHP (10-acetyl-3,7-dihydroxyphenoxazine) to produce the highly fluorescent compound resorufin. Resorufin fluorescence can be analyzed with an excitation wavelength between 530-540 nm and an emission wavelength between 585-595 nm. Alternatively, the absorbance of resorufin can be measured at 570 nm.
Cayman Chemical PhagocytosIs asy KItIgG F 1ea
Cayman’s Phagocytosis Assay Kit (IgG FITC) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the advantage of visualizing perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations. This kit provides enough Latex Beads-Rabbit IgG-FITC Complex for up to 750 samples.
Cayman Chemical cPLA2 asy KIt 96Wel
Arachidonoyl thio-PC is a substrate for cPLA2 by virtue of the presence of arachidonic acid at the sn-2 position of the glycerophospholipid. Hydrolysis of the arachidonoyl thioester bond at the sn-2 position by PLA2 releases free thiol which can be detected by DTNB. This assay can be used to determine the activity of cPLA2 in purified preparations, cell cultures, or tissue homogenates that are known to contain only cPLA2. Use of this assay with preparations containing more than one type of PLA2 will result in the measurement of total PLA2 activity rather than cPLA2 alone. Isozyme-specific cPLA2 activity can be measured by excluding sPLA2 or inhibiting iPLA2 activities in the assay.
Dojindo Molecular Technologies Inc JC-1 MitoMP Detection Kit (1 set), easy to dissolve, can be detected by several instruments (Ex=500 nm, Em=530 nm), Imaging Buffer included: keeps the cell condition optimal for a long period.
Mitochondria is one of the important organelles in discussing early cytotoxicity, oxidative stress, and apoptosis in vivo. Mitochondria synthesize ATP using oxygen to produce the necessary energy for living cells. The lowering or dysfunction of mitochondrial activity is known to be closely related to cancer, aging, and neurodegenerative diseases such as Alzheimer’s and Parkinson’s diseases. JC-1 is widely used for observing mitochondrial membrane potential. JC-1 forms aggregates (in healthy mitochondria) with red fluorescence (590 nm). As membrane potential decreases, JC-1 dissociates into monomers, which show green fluorescence (530 nm).Unlike other commercially available JC-1, no precipitation of JC-1 aqueous buffer occurs by using this kit. In addition, the Image Buffer included in this kit minimizes fluorescent background and maintains cell health during assays. Dojindo offers various reagents for mitochondria research. (https://www.dojindo.com/mitochondria-research/)
Dojindo Molecular Technologies Inc Cellular Senescence Detection Kit (10 plate), detects SA-β-gal with high sensitivity by SPiDER-βGal, applicable to both living and fixed cells, by fluorescence microscopy or flow cytometry.
SPiDER-βGal is a new reagent to detect β-galactosidase specifically which possesses a high cell-permeability and a high retentivity inside cells. This novel reagent can detect β-galactosidase not only in fixed cells but also in living cells, and also can be applied to quantitative analysis by flow cytometry. Senescence-associated beta-galactosidase (SA-β-gal), which is overexpressed in senescent cells, has been widely used as a marker of cellular senescence. Cellular Senescence Detection Kit – SPiDER-βGal is optimized to detect intracellular SA-β-gal. Modern publications with a high number of citations summarize the various features of cellular senescence on the following page (https://www.dojindo.com/senescent-cell-detection-selection-guide/).
Cell Biolabs Inc OxiSelect™ TBARS Assay Kit, 200 assays
Thiobarbituric Acid Reactive Substances (TBARS) is a well-established assay for screening and monitoring lipid peroxidation. The rapid and easy protocol has been modified by researchers in the evaluation of drugs, food, as well as human and animal tissue samples. MDA forms a 1:2 adduct with thiobarbituric acid. The MDA-TBA adduct formed from the reaction of MDA in samples with TBA can be measured colorimetrically or fluorometrically. TBARS levels are determined from a Malondialdehyde equivalence standard. The OxiSelect™ TBARS Assay Kit offers a simple, reproducible, and consistent system for the detection of lipid peroxidation in urine, plasma, serum, lysates, and tissue homogenates. This kit includes an MDA standard for use as a positive control. Each kit provides sufficient reagents to perform 200 tests including standard curve and unknown samples.
Cell Biolabs Inc ViraDuctin™ AAV Transduction Kit, 50 transductions
AAV can infect both dividing and non-dividing cells and can be maintained in the human host cell, creating the potential for genome integration. Because AAV is a naturally defective virus, requiring provision of several factors in trans for productive infection, it is considered the safest viral vector to use. The AAV transduction process includes viral binding and entry, intracellular trafficking, nuclear transport, and viral second strand DNA synthesis. The viral second strand DNA synthesis has been shown to be the rate limiting step, which leads to inefficient transduction by AAV vectors. ViraDuctin™ AAV Transduction Kit is a proprietary formulation designed to increase the transduction efficiency of AAV vectors in a variety of cell types. This kit provides the following advantages: (1) Higher transduction efficiency in many cell types, (2) Easy to use, and (3) It is ideal for transduction of nonpermissive cells such as primary cells and stem cells. 50 transductions/kit.
Cayman Chemical -NAD sodIum salt 6.9mg
A fluorescent probe for NAD+-dependent enzymes; ex/em = 300/410 nm, respectively
Cayman Chemical LIpIdLaunchLNP102 Uptake K 1ea
Cayman’s LipidLaunch™ LNP-102 Uptake Kit (Near-Infrared Fluorescence) is intended to serve as a starting point for laboratories to explore the uptake of LNPs in vivo without the need for specialized equipment. Optimal preparation conditions for the encapsulation of nucleic acids with LNPs must be determined by the end user.
Cell Biolabs Inc Total Bile Acid Assay Kit (Fluorometric), 100 assays
Determining circulatory levels of bile acids can be used to identify or diagnose certain liver diseases. In addition, elevated serum bile levels have been observed in intrahepatic cholestasis of pregnancy cases. Cell Biolabs’ Total Bile Acid Assay Kit is a simple fluorometric assay that measures the amount of total bile acid present in plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. The assay is based on an enzyme driven reaction: when bile acids are incubated in the presence of 3α-hydroxysteroid dehydrogenase (3α-HSD) and NAD+, NAD+ is converted to its reduced form NADH. Diaphorase then uses NADH to reduce resazurin to resorufin which is then detected fluorometrically. Sample bile acid concentrations are determined by comparison with a known bile acid standard. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, bile acid standards and unknown samples.