General Cell Based Assays
ELABSCIENCE Alanine Aminotransferase (ALT/GPT) Activity Assay Kit, 100 Assays
ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37°C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
Cayman Chemical MitoLite™ Orange FX570, 500 test
A mitochondrial dye for live and fixed cells; ex/em = 545/575 nm; has been used to label mitochondria in Hep2 cells treated with cisplatin and NAC
Enzo Life Sciences LYSO-ID Red cytotoxicity kit (GFP-CERTIFIED) (1Kit)
The LYSO-ID Red Cytotoxicity Kit monitors dysfunction of lysosomal degradation using a drug-like cationic amphiphilic tracer (CAT) dye that rapidly and selectively stains acidic organelles, and is suitable for monitoring accumulation of lysosomes and lysosome-like structures in live cells. Mechanism of Action A cationic amphiphilic tracer (CAT) dye that rapidly partitions into cells in a similar manner as drugs that induce phospholipidosis. This tracer was created by placing titratable groups on the probe (dye), to enable labeling to be expanded into lamellar inclusion bodies of cells pretreated with weakly basic cell-permeant compounds. Besides lysosomes themselves, the probe can be employed for highlighting lysosome-like organelles under conditions wherein cells produce vacuoles that contain most of the degradative enzymes of the lysosome but are not as acidic as the parent organelle. Applications: Fluorescence microscopy, Fluorescent detection .
Eurofins Discoverx cAMP Hunter™ Human TSHR(L) Gs Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human TSHR(L) Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure TSHRL (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical Hydroxyproline Assay Reagent 2, 14 mL
Hydroxyproline Assay Reagent 2 has been tested and formulated to work exclusively with Cayman’s Total Collagen/Hydroxyproline Assay Kit (Item No. 702440).
Eagle Biosciences Inc Ultra Sensitive Colorimetric Assay for Nitric Oxide Synthase
Ultra Sensitive Colorimetric Assay for Nitric Oxide Synthase
Eagle Biosciences Inc Nitric Oxide Synthase Non-Enzymatic Assay Kit
Nitric Oxide Synthase Non-Enzymatic Assay Kit
Eurofins Discoverx cAMP Hunter eXpress VIPR2 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0052E2CP2L)
The cAMP Hunter VIPR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. VIP) based activation of VIPR2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type VIPR2, a naturally Gs coupled GPCR. Upon activation of VIPR2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 23 Reagent, 500. (93-0563R23B)
AssayComplete Cell Plating 23 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx cAMP Hunter eXpress ADRA2C CHO-K1 GPCR Assay, 2-Plate Kit. (95-0053E2CP2M)
The cAMP Hunter ADRA2C eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2C GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRA2C, a naturally Gi/Go coupled GPCR. Upon activation of ADRA2C, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human EGFR ASY
The R-PLEX Human EGFR Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay measuring total EGFR levels in human serum plasma and cell culture supernatants This electrochemiluminescence (ECL) assay uses MSD s SULFO-TAG labels and multi-array plate technology for simultaneous detection of multiple protein biomarkers from a single sample It offers high sensitivity broad dynamic range low sample volume and rapid results The kit includes a 96-well plate with capture antibody detection antibody calibrator and assay diluent suitable for biomarker discovery disease research and drug development
Eurofins Discoverx PathHunter eXpress CSF1R/CSF1R Dimerization Assay, 2pl Kit. (93-1061E3CP5M)
The PathHunter CSF1R/CSF1R Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. M-CSF) induced dimerization of CSF1R/CSF1R receptors. This product uses EFC technology. The cell line included overexpresses CSF1R target protein fused to PK alongside CSF1R target protein fused to EA. Upon ligand-induced dimerization of CSF1R/CSF1R, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter Teriparatide Bioassay Kit, 10pl. (95-0118Y2-00058)
The cAMP Hunter Teriparatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Teriparatide based activation of PTHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR, a naturally Gs coupled GPCR. Upon activation of PTHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Molecular Devices FLIPR Calcium 6-QF Assay Kit, Quench-free, 10 plates
The FLIPR Calcium 6-QF Assay Kit (Explorer kit) is a quench-free, mix-and-read fluorescent assay for measuring intracellular calcium flux in high-throughput plate formats. The Calcium 6-QF formulation provides an increased signal window and is suitable for quench-sensitive targets and multiplexed assays.
- Quench-free formulation for quench-sensitive targets and multiplexing.
- Designed for mix-and-read workflows with pre-optimized FLIPR and FlexStation protocols.
- Sufficient reagents for 10 plates in Explorer kit format.
- Each reagent vial for Sample and Explorer kits is sufficient for one 96-, 384-, or 1536-well plate.
- Suitable for endogenous, primary, and stem cell targets with improved signal window and assay robustness.
Eurofins Discoverx PathHunter eXpress OPRK1 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0234E3CP5M)
The PathHunter OPRK1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dynorphin A) based activation of OPRK1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPRK1 and EA-tagged β-Arrestin-2. Activation of OPRK1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.