General Cell Based Assays
Abcam GSK-J5, Inactive control for GSK-J4, 10MG
MW 453.97 g/mol, Purity >97%. Cell-permeable, inactive control for histone demethylase JMJD3/UTX inhibitor GSK-J4 (ab144395). Active in vitro.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress HRH4 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0209E3CP0M)
The PathHunter HRH4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. histamine) based activation of HRH4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HRH4 and EA-tagged β-Arrestin-2. Activation of HRH4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
NEOGEN Cellulase Assay Kit, Colorimetric Method, 120-240 Manual Assays, 480 Auto-Analyser Assays, pH 4.5 to 8.0 (or pH 11.0), Temperature Up to 60°C
Cellulase Assay Kit, Colorimetric Method, 120-240 Manual Assays, 480 Auto-Analyser Assays, pH 4.5 to 8.0 (or pH 11.0), Temperature Up to 60°C
Revvity Health Sciences Inc HTRF Human & Mouse PARP cleaved-Asp214 Detection Kit, Control Lysate
HTRF Human & Mouse PARP cleaved-Asp214 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-VASP (Ser157) Detection Kit, Control Lysate
HTRF Human & Mouse Phospho-VASP (Ser157) Detection Kit, Control Lysate
Abcam Mevastatin, HMG-CoA reductase inhibitor, 50MG
MW 390.5 Da, Purity >98%. Potent HMG-CoA reductase inhibitor. Proapoptotic, able to arrest cells in G₁ by down-regulation of CDK4, CDK6 and cyclin D1 and up-regulation of p21 and p27.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress CCR4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0193E2CP0M)
The PathHunter CCR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL22/MDC) based activation of CCR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR4 and EA-tagged β-Arrestin-2. Activation of CCR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical PAF Acetylhydrolase Assay Kit, 96 Well
PAF-AH catalyzes the hydrolysis of the potent biologically-active phospholipid PAF, generating inactive lyso-PAF. Cayman’s PAF-AH assay kit provides an accurate and convenient method for measurement of PAF-AH activity. The assay uses 2-thio PAF which serves as a substrate for PAF-AH. Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using Ellman’s reagent.
Eurofins Discoverx cAMP Hunter™ Human Glucagon (GCG Receptor) Gs Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human Glucagon (GCGR) Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GCGR (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical Cysteinyl Leukotriene Express ELISA Kit, 96 solid
Cayman’s CysLT Express ELISA Kit is a competitive assay that can be used for quantification of CysLTs in urine, plasma, serum, lavage fluids and aspirates, culture media, and other sample matrices. The assay has a range of 7.8-1,000 pg/ml, an average sensitivity (80% B/B0) of 24 pg/ml, and a lower limit of detection (LLOD) of 6.4 pg/ml. NOTE: This kit measures all CysLTs. For measurement of specific CysLTs, please see Cayman Chemical’s Leukotriene E4 ELISA Kit (Item No. 501060) and Leukotriene C4 ELISA Kit (Item No. 501070).
Eurofins Discoverx AssayComplete Cell Culture Kit-110, (92-3110G)
AssayComplete Cell Culture Kit-110 is an optimized media kit intended for use in culturing and propagating Eurofins DiscoverX cell lines. This kit contains the base medium, relevant serum, and pen/strep required to produce a full cell culture medium for optimal cell culture results. This product is provided as a 3-component kit which will produce approximately 550 mL of finished media. Please refer to cell line specific datasheet to determine the recommended cell culture kit. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Meso Scale Discovery U-PLEX Human ApoA1 ASY
The Meso Scale Discovery U-PLEX Human ApoA1 Assay Kit is a multiplex immunoassay that measures Apolipoprotein A1 (ApoA1) levels in human serum plasma and cell culture supernatant samples This assay utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision The kit includes a 96-well plate coated with capture antibody detection antibody Read Buffer Calibrator Diluent and lyophilized U-PLEX Calibrator This quantitative immunoassay allows users to accurately determine ApoA1 concentrations within 90 minutes using as little as 25 L of sample
Enzo Life Sciences sPLA2 activity kit (1x96wells)
The sPLA2 activity kit is a higher throughput assay for the quantification of up to 42 samples in duplicate per 96 well kit. This kit provides safe, sensitive, and reliable results in under an hour. Alternative Name: Secretory phospholipase A2. Sensitivity: 2.34 Units/ml (range 5 - 80 Units/mL). Applications: Colorimetric detection, Activity assay. Contents: Microtiter plate, Standard, Reaction buffer concentrate, Substrate, Stop reagent, Color reagent
Eurofins Discoverx Calcium No WashPLUS, 10 plates (384-well). (90-0091)
The Calcium No WashPLUS detection kit is intended for use with cells expressing receptors that signal through calcium. The kit contains a calcium-sensitive dye which assays intracellular calcium levels upon activation of a Gαq-coupled GPCR. Activation of the GPCR leads to release of intracellular calcium, and a detectable increase in fluorescent signal. This detection kit contains reagents for assaying 10 plates. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/cell-line/calcium-no-washplus-90-0091.
Abcam HDAC substrate, colorimetric (Boc-Lys(Ac)-pNA), 25MG
MW 408.45 g/mol, Purity >98%. Colorimetric substrate for histone deacetylases (HDAC). For use in HDAC activity assays.
The product is subject to the following: Abcam Restricted Use Statement