General Cell Based Assays
AURORA BIOLABS LLC Cov-19 NP Rabbit Binding Assay, 384 rxn
SARS-CoV-2 Nucleocapsid protein (NP) is one of the core components of SARS-CoV-2 virus. It forms a complex with viral genomic RNA in a helical symmetrical structure and plays a key role in the process of virus replication and assembly. Since NP is abundantly expressed during infection, it can be used as an important diagnostic marker for COVID-19 and also can be used as a potential drug target or developing vaccines. Terbium-labeled anti-Tag1 antibody serves as fluorescence donor, that binds to the His-Tagged NP. If a test rabbit antibody binds to NP, fluorescence- labeled anti-rabbit antibody (fluorescence acceptor) will be brought in close proximity with the fluorescence donor. Excitation of Terbium (340 nm) generates fluorescence resonance energy transfer (FRET) to the fluorescence-labeled acceptor, which consequently fluoresces at 665 nm (figure below). Thus, the test antibody binding to NP can be quantitively measured by calculation of the fluorescent ratio of 665 nm/620 nm.
Eurofins Discoverx cAMP Hunter™ Human RXFP4 Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human RXFP4 Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure RXFP4 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human TORC3 (CRTC3) Nuclear Translocation Cell-Based Assay Kit (U2OS)
The PathHunter Human TORC3 (CRTC3) Stable Cell Line Nuclear Translocation Assay (U2OS) contains a stable clonal cell line used to measure CRTC/CREB pathway activation via nuclear translocation of TORC3. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human Apelin (APJ Receptor) Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human Apelin (APJ Receptor) Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure AGTRL1 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam Triglyceride Assay Kit (Fluorometric, Reducing Samples)
Triglyceride Assay Kit (Fluorometric, Reducing Samples)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress BAI2 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0546E2CP1L)
The PathHunter BAI2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of BAI2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BAI2 and EA-tagged β-Arrestin-2. Activation of BAI2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical HS MInral OIl asy Reagent 15mL
A kainate receptor agonist induces currents in Xenopus oocytes expressing homomeric GluR5 or GluR2 subunit-containing receptors (EC50s 0.16 and 0.23 UM respectively) induces desensitization in HEK293 cells expressing homomeric GluR6 subunit-containing kainate receptors at 0.03-0.3 UM selectively binds to the kainate receptor (IC50 ~0.035 UM) over the AMPA and NMDA receptors (IC50s >100 and 7 UM respectively) but does bind to EAAT2 (Kb 3.4 UM) and inhibits glutamate transport reduces hind paw withdrawal frequency and increases withdrawal latency to mechanical and thermal stimuli in a rat model of capsaicin-induced hyperalgesia at 10 50 and 100 mg/kg
Meso Scale Discovery U-PLEX Mouse IL-13 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-13 Assay Kit is a multiplex immunoassay that allows simultaneous measurement of mouse interleukin 13 (IL-13) in serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision The kit includes a plate coated with capture antibodies detection antibodies labeled with an ECL compound calibrators controls 10X diluent read buffer wash buffer and adhesive plate seals The assay has a typical sensitivity of 0 3 pg/mL and an upper limit of quantification of 10 000 pg/mL for mouse IL-13
Meso Scale Discovery U-PLEX Human TNF-B ASY
The Meso Scale Discovery U-PLEX Human TNF-beta Assay Kit is a multiplex immunoassay that measures TNF-beta levels in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay has high specificity and low cross-reactivity It includes a 96-well plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent tags calibrators and buffer The kit allows simultaneous measurement of TNF-beta and other biomarkers It can be used for biomarker discovery lead optimization and diagnostics
BioVendor, LLC Sircol; Dye & Dye Release Reagents (2 x 110 ml, 2 x 150 ml). CONTAINS: Sircol; Dye Reagent (2 x 110 ml) AND Sircol™ Dye Release Reagents (2 x 150 ml). Biocolor Item # S1500
Sircol; Dye & Dye Release Reagents (2 x 110 ml, 2 x 150 ml). CONTAINS: Sircol; Dye Reagent (2 x 110 ml) AND Sircol™ Dye Release Reagents (2 x 150 ml). Sircol™ Collagen Assay components. Biocolor Item # S1500
Cayman Chemical Ascorbate Assay Kit, 96 Well
Ascorbate (L-Ascorbic acid or Vitamin C) is a six-carbon lactone that is synthesized from glucose in the liver of most mammalian species, but not by humans who must obtain ascorbate through dietary consumption. In humans, ascorbate serves as an antioxidant and acts as an electron donor for various enzymes. Cayman’s Ascorbate Assay provides a reproducible, sensitive fluorescence-based tool for quantifying ascorbate from plasma, serum, urine, and fruit juices.
Cayman Chemical Aconitase Assay Kit, 96 Well
Aconitase is an iron-sulfur protein containing a [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate. Whereas exposure of aconitase to oxidants renders the enzyme inactive, loss of aconitase activity in cells or in biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. Cayman’s Aconitase Assay provides a simple, reproducible, and sensitive tool for assaying aconitase from tissue homogenates or cell lysates.
Abcam Intracellular glutathione (GSH) Detection Assay Kit
Intracellular glutathione (GSH) Detection Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress DRD5 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0426E2CP0M)
The PathHunter DRD5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DRD5 and EA-tagged β-Arrestin-2. Activation of DRD5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.