Formulated to deliver the fastest amplification speed (time-to-results), yield, salt tolerance and sensitivity, High Conc. Glycerol-Free Bst contains 5´- 3´ DNA polymerase activity and strong strand displacement activity but lacks 5´- 3´ exonuclease activity making it an ideal enzyme for isothermal amplification, including HDA, MCA and Loop-Mediated Isothermal DNA Amplification (LAMP). It is also lyophilization-compatible, making it ideal for point of care test (POCT) assays that require ambient temperature shipping and storage. For the most difficult templates, an Inhibitor-Tolerant Bst Buffer (MDX019) is available that is specifically designed for high performance when using crude samples such as sputum or saliva.