Restriction Enzymes
New England Biolabs, Inc. PmeI – 500 units
A restriction endonuclease that recognizes the sequence GTTT_^AAAC.
New England Biolabs, Inc. XhoI – 25000 units
A restriction endonuclease that recognizes the sequence C^TCGA_G.
New England Biolabs, Inc. PaqCI - 200 units
PaqCI is a Type IIS restriction enzyme with a 7 basepair recognition sequence: CACCTGC(4/8). It is sourced from an E. coli strain that carries the modified cloned PaqCI gene from Paucibacter aquatile. PaqCI requires multiple sites for efficient cleavage -- PaqCI Activator is included for optimal cleavage. This enzyme is supplied with NEB's rCutSmart Buffer and is heat inactivated.
New England Biolabs, Inc. ScaI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence AGT^ACT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
New England Biolabs, Inc. PacI – 1250 units
A restriction endonuclease that recognizes the sequence TTA_AT^TAA.
Apexbio Technology LLC Dpn I(Synonyms: DpnI, DpnI Restriction Enzyme, Dam methylation-sensitive restriction enzyme DpnI), 5000U.
Dpn I is a restriction endonuclease sensitive to Dam methylation specifically recognizing and cleaving the adenine N6-methylated sequence GA/TC within DNA Cleavage is dependent on adenine methylation status mammalian CpG methylation overlapping Dpn I sites blocks cleavage and methylation by Dcm is not recognized by this enzyme It is routinely used for the rapid digestion of plasmid DNA PCR fragments and genomic DNA and finds broad utility in genetic typing molecular cloning Southern blotting SNP genotyping and restriction fragment length polymorphism (RFLP) analyses
Vector Biolabs SRF-Luc Adenovirus (Ad-SRF-Luc)
Expression of the Photinus pyralis (firefly) luciferase gene is controlled by a synthetic promoter that contains direct repeats of the transcription recognition sequences for the serum response factor (SRF).When this Luc reporter adenovirus and an uncharacterized gene are cotransfected into mammalian cells, the activation of endogenous protein kinases initiated by the cotransfected gene product will result in the activation of corresponding trans-activators which in turn stimulate luciferasae expression.
New England Biolabs, Inc. ApoI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence R^AATTY. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. PI-PspI – 500 units
A restriction endonuclease that recognizes the sequence TGGCAAACAGCTA_TTAT^GGGTATTATGGGT.PI-PspI is obtained from a strain of E. coli which expresses the DNA polymerase from the extreme thermophile, Pyrococcus species GB-D (1). PI-PspI is a product of in vivo protein splicing that gives rise to both polymerase and endonuclease from a single polypeptide precursor.
New England Biolabs, Inc. PvuII – 5000 units
A restriction endonuclease that recognizes the sequence CAG_^CTG.
New England Biolabs, Inc. AvaII – 2000 units
A restriction endonuclease that recognizes the sequence G^GWC_C.
New England Biolabs, Inc. EcoRV-HF – 20000 units
A high fidelity restriction endonuclease that recognizes the sequence GAT_^ATC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Cayman Chemical Myrca rubra bark Myrctrn 50mg
A flavonoid with diverse biological activities; active against B. subtilis, E. coli, K. pneumoniae, S. aureus, and C. albicans (MIC = 0.25 mg/ml for all); inhibits AChE and α-glucosidase in vitro (IC50s = 65 and 79 μg/ml, respectively); inhibits LPS-induced production of TNF-α and enhances LPS-induced RANTES production in RAW 264.7 cells at 0.2 and 1 mM