Restriction Enzymes
ABclonal Technology BstBI
5...T T/ C G A A...3 3...A A G C/ T T...5 Isoschizomers: Bsp119I AsuII Bpu14I BspT104I NspV SfuI
New England Biolabs, Inc. APE 1 – 1000 units
Human apurinic/apyrimidinic (AP) endonuclease, APE 1, also known as HAP 1 or Ref-1, shares homology with Escherichia coli exonuclease III protein. APE 1 cleaves the phosphodiester backbone immediately 5 to an AP site, via hydrolytic mechanism, to generate a single-strand DNA break leaving a 3-hydroxyl and 5-deoxyribose phosphate terminus. Besides AP endonuclease activity, APE 1 has also been reported to have weak DNA 3 -diesterase, 3 to 5 exonuclease and RNase H activities (3-5). In addition to DNA repair activity, APE 1 is also capable of regulating the DNA binding activity of many transcription factors in vitro by a redox mechanism. As part of this process, APE 1 has been shown to stimulate the DNA binding activity of Fos-Jun heterodimers, Jun-Jun homodimers and Hela cell AP-1 proteins as well as that of several other transcription factors including NF-kB, Myb and members of the ATF/CREB family.
New England Biolabs, Inc. XbaI – 3000 units
A restriction endonuclease that recognizes the sequence T^CTAG_A.
New England Biolabs, Inc. I-CeuI – 500 units
A restriction endonuclease that recognizes the sequence CGTAACTATAACGGTC_CTAA^GGTAGCGAA.The intron encoding I-CeuI is present in the chloroplast large rRNA gene of Chlamydomonas eugametos (1). This gene has been cloned and overexpressed in E. coli (2).
New England Biolabs, Inc. SacI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G_AGCT^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. EnGen Spy Cas9 Nickase – 70 pmol
EnGen Spy Cas9 Nickase is a variant of Cas9 nuclease differing by a point mutation (D10A) in the RuvC nuclease domain, which enables it to nick, but not cleave, DNA). EnGen Spy Cas9 Nickase, like EnGen Cas9 NLS , targets DNA using a guide RNA complementary to a site with a 3' NGG protospacer adjacent motif (PAM). Nicking occurs on the DNA strand opposite the PAM. Double-stranded DNA breaks can be generated with reduced off-target cleavage by targeting two sites in close proximity (generally 0-20 bp apart) and with PAMs facing outward to leave 5' overhangs. Nicking by EnGen Spy Cas9 Nickase occurs on the DNA strand opposite the target sequence. EnGen Spy Cas9 Nickase contains SV40 T antigen nuclear localization (NLS) sequences on the N and C- termini.